NBDC Research ID: hum0079.v1
SUMMARY
Aims: To elucidate the pathogenesis of inclusion body myositis
Methods: RNA-sequencing analysis in 53 patients with inclusion body myositis, 6 patients with polymyositis, and 9 controls
Participants/Materials: Patients with inclusion body myositis, patients with polymyositis, and controls/Muscle biopsy samples
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000068 | NGS (RNA-seq) | Controlled-access (Type I) | 2022/02/18 |
*Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more
MOLECULAR DATA
| Participants/Materials |
inclusion body myositis (ICD10: G724): 53 cases polymyositis (ICD10: M332): 6 cases 9 controls |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2500] |
| Library Source | RNAs extracted from skeletal muscle |
| Cell Lines | - |
| Library Construction (kit name) | TruSeq Stranded Total RNA with ribo-zero gold |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 101 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000068 |
| Total Data Volume | 721.5 GB (fastq) |
| Comments (Policies) | NBDC policy |
This study was supported by “Genome Science”. “Genome Science” is a part of the “Support Programs for Three Fields in Life Sciences (cancer, genome and brain sciences)” established by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) under the Grant-in-Aid for Scientific Research on Innovative Areas.
DATA PROVIDER
Principal Investigator: Jun Goto
Affiliation: Department of Neurology, The University of Tokyo, Graduate School of Medicine
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (C) | Expression profiling study in inclusion body myositis employing next-generation sequencer | 26461265 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Muscle transcriptomics shows overexpression of cadherin 1 in inclusion body myositis | doi: 10.1002/ana.26304 | JGAD000068 |
| 2 |
USERS (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
| Pietro Fratta | University College London | United Kingdom of Great Britain and Northern Ireland | TDP-43 pathology and loss of function in the skeletal muscle | JGAD000068 | 2022/06/07-2025/02/28 |
| Michiaki Hamada | Faculty of Science and Engineering, Waseda University | Japan | Construction of RNA-targeted Drug Discovery Database | JGAD000068 | 2022/12/26-2025/03/31 |
