NBDC Research ID: hum0068.v3
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SUMMARY
Aims: Establishing a method for detecting variations of lung adenocarcinoma
Methods: [PCR amplicon Seq] Amplicon sequencing by using of MinION (Nanopore)
[WGS] Whole-genome sequencing for identification and characterization of a novel class of structual aberrations in cancers by using of NovaSeq and PromethION.
[RNA-seq] RNA sequencing by using of NovaSeq and PromethION.
Participants/Materials: Lung adenocarcinoma
URL: http://kero.hgc.jp/
| Data Set ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000065 | NGS (PCR amplicon Seq) | Controlled Access (Type I) | 2017/07/10 |
| JGAS000065 | Controlled Access (Type I) | 2020/10/19 | |
| JGAS000349 | Controlled Access (Type I) | 2021/12/14 |
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MOLECULAR DATA
| Participants/Materials | lung adenocarcinoma: 8 cases (7 samples) |
| Targets | PCR amplicon Seq |
| Target Loci for Capture Methods |
sample 1: Amplicons from EML4-ALK positive case (EML4-ALK region) and KIF5B-RET positive case (KIF5B-RET region) were mixed (1 sample) sample 2 - 7: Amplicons from 6 cases (EGFR and KRAS regions) |
| Platform | Nanopore [MinION] |
| Library Source | cDNA were synthesized from mRNA extracted from lung adenocarcinomas |
| Cell Lines | - |
| Library Construction (kit name) | Nanopore Sequencing Kit (Oxford Nanopore Technologies) |
| Fragmentation Methods | PCR |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 367 Mb |
| Japanese Genotype-phenotype Archive Data set ID | JGAD000065 |
| Total Data Volume | 889 MB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
lung adenocarcinoma (ICD10: C349): 21 cases (42 samples) tumor tissues: 21 samples normal tissues: 21 samples |
| Targets | WGS |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | DNAs extracted from tumor and normal tissues of lung adenocarcinomas |
| Cell Lines | - |
| Library Construction (kit name) | TruSeq Nano DNA Library Prep kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 2.8 Tb |
| Japanese Genotype-phenotype Archive Data set ID | JGAD000252 |
| Total Data Volume | 2 TB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
lung adenocarcinoma (ICD10: C349): 21 cases (41 + 8 samples) tumor tissues: 21 + 4 samples normal tissues: 20 + 4 samples |
| Targets | WGS |
| Target Loci for Capture Methods | - |
| Platform | Nanopore [PromethION] |
| Library Source | DNAs extracted from tumor and normal tissues of lung adenocarcinomas |
| Cell Lines | - |
| Library Construction (kit name) | SQK-LSK109 |
| Fragmentation Methods | - |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 2.7 Tb |
| Japanese Genotype-phenotype Archive Data set ID | |
| Total Data Volume | 2.4 + 1.4 TB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
lung adenocarcinoma (ICD10: C349): 6 cases (12 samples) tumor tissues: 6 samples normal tissues: 6 samples |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Nanopore [PromethION] |
| Library Source | RNAs extracted from tumor and normal tissues of lung adenocarcinomas |
| Cell Lines | - |
| Library Construction (kit name) | SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, SQK-LSK109 |
| Fragmentation Methods | - |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 850 bp on average |
| Japanese Genotype-phenotype Archive Data set ID | JGAD000463 |
| Total Data Volume | 1.4 TB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
lung adenocarcinoma (ICD10: C349): 6 cases (12 samples) tumor tissues: 6 samples normal tissues: 6 samples |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from tumor and normal tissues of lung adenocarcinomas |
| Cell Lines | - |
| Library Construction (kit name) | SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing、Nextera XT DNA Library Preparation Kit |
| Fragmentation Methods | Tagmentation (Nextera XT DNA Library Preparation Kit) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 300 bp |
| Japanese Genotype-phenotype Archive Data set ID | JGAD000463 |
| Total Data Volume | 1.4 TB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Takashi Kono
Affiliation: Division of Genome Biology, National Cancer Center Research Institute
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas | Platform for Advanced Genome Science | 16H06279 |
| KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas | Conquering cancer through neo-dimensional systems understanding; Sequencing and phasing cancer mutations using a nanopore sequencer MinION | 16H01582 |
| Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED) | Stratification of cancer patients by novel genomic aberrations using long-read sequencing technologies | 19cm0106539h0002 |
| Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED) | Study of genomic evolution of non-small cell lung cancers using long read sequencing technologies | JP21cm0106582 |
PUBLICATIONS
| Title | DOI | Data Set ID | |
|---|---|---|---|
| 1 | Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer. | doi: 10.1093/dnares/dsx027 | JGAD000065 |
| 2 | Long-read sequencing for non-small-cell lung cancer genomes | doi: 10.1101/gr.261941.120 |
JGAD000252 JGAD000253 |
| 3 |
USRES (Controlled-Access Data)
| Principal Investigator | Affiliation | Research Title | Data in Use (Data Set ID) | Period of Data Use |
|---|---|---|---|---|
