NBDC Research ID: hum0068.v1

SUMMARY

Aims: Establishing a method for detecting variations of lung adenocarcinoma

Methods: Amplicon sequencing by using of MinION (Nanopore)

Participants/Materials: Lung adenocarcinoma

Data Set ID	Type of Data	Criteria	Release Date
JGAS000065	NGS (PCR amplicon Seq)	Controlled Access (Type I)	2017/07/10

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MOLECULAR DATA


Participants/Materials	lung adenocarcinoma: 8 cases (7 samples)
Targets	PCR amplicon Seq
Target Loci for Capture Methods	sample 1: Amplicons from EML4-ALK positive case (EML4-ALK region) and KIF5B-RET positive case (KIF5B-RET region) were mixed (1 sample) sample 2 - 7: Amplicons from 6 cases (EGFR and KRAS regions)
Platform	Nanopore [MinION]
Library Source	cDNA were synthesized from mRNA extracted from lung adenocarcinomas
Cell Lines	-
Library Construction (kit name)	Nanopore Sequencing Kit (Oxford Nanopore Technologies)
Fragmentation Methods	PCR
Spot Type	Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	367 Mb
Japanese Genotype-phenotype Archive Data set ID	JGAD000065
Total Data Volume	889 MB (fastq)
Comments (Policies)	NBDC policy

Principal Investigator: Takashi Kono

Affiliation: Division of Genome Biology, National Cancer Center Research Institute

Project / Group Name: -

Funds / Grants (Research Project Number):

Name	Title	Project Number
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas	Platform for Advanced Genome Science	16H06279
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas	Conquering cancer through neo-dimensional systems understanding; Sequencing and phasing cancer mutations using a nanopore sequencer MinION	16H01582

	Title	DOI	Data Set ID
1	Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer.	doi: 10.1093/dnares/dsx027	JGAD000065
2

Principal Investigator:	Affiliation:	Data in Use (Data Set ID)	Period of Data Use