hum0032 Release Note
| Research ID | Release Date | Type of Data |
|---|---|---|
| hum0032.v1 | 2016/07/22 | NGS (PBAT-seq, ChIP-seq, RNA-seq) |
hum0032.v1
NGS (PBAT-seq)
gDNAs extracted from normal and diseased human hepatocytes purified from partial hepatectomy specimens were used for analyses (fastq format files). After library preparation by post-bisulfite adaptor-tagging (PBAT, http://crest-ihec.jp/english/epigenome/index.html) and multiplex sequence was performed with the Illumina HiSeq 2000 sequencer (100 bp, Single-end).
NGS (ChIP-seq)
Anti H3K4me3,H3K9me3, H3K27me3, H3K27ac, H3K4me1 and H3K36me3 mouse monoclonal antibodies, kindly provied Dr. Hiroshi Kimura, Tokyo Institute of Technology, were used for immunoprecipitation. ChIP-Seq libraries were sequenced using the HiSeq 2000 or 2500 (Illumina) platform.
NGS (RNA-seq)
Total RNAs extracted from normal and diseased human hepatocytes purified from partial hepatectomy specimens were used for whole transcriptome-sequencing analysis (fastq format files). cDNAs were prepared using the TruSeq RNA Library Preparation Kit v2 (Illumina) or SureSelect Strand Specific RNA kit (Agilent) and multiplex sequence was performed with the Illumina HiSeq 2000 sequencer (100 bp, Paired-end).
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