NBDC Research ID: hum0022.v1
SUMMARY
Aims: To clarify the molecular mechanism underlying malignant transformation of Donor-cell derived leukemia (DCL).
Methods: Whole Exome Sequencing was performed by using of Illumina HiSeq 2000.
Participants/Materials: One DCL patient and one donor
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000017 | NGS (Exome) | Controlled-access (Type I) | 2015/02/02 |
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MOLECULAR DATA
| Participants/Materials | One DCL patient (cells were obtained at the points of initial acute myeloid leukemia, remission stage, relapsed stage, and DCL at late stage). One donor (normal peripheral blood) |
| Targets | Exome |
| Target Loci for Capture Methods |
- |
| Platform | Illumina [HiSeq 2000] |
| Library Source | Genomic DNAs were extracted from leukemic cells from bone marrow aspirate or peripheral blood. |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Kit (Agilent) |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris S220) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000017 |
| Total Data Volume | 500 GB (bam [ref: GRCh37]) |
| Comments (Policies) |
DATA PROVIDER
Principal Investigator: Hitoshi Kiyoi
Affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine
Project / Group Name: Project for Development of Innovative Research on Cancer Therapeutics , Team for Strategic Therapy Design
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
|
Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT) |
Therapy 1 : Establishment of the strategic therapy design based on the development of molecular-targeted drugs |
Therapy 1 - ① : Establishment of the optimal remedy based on the leukemia genome study |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Leukemic evolution of donor-derived cells harboring IDH2 and DNMT3A mutations after allogeneic stem cell transplantation | doi:10.1038/leu.2013.278 | JGAD000017 |
| 2 |
USERS (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
| Seishi Ogawa | Kyoto University | JGAD000017 | 2015/12/22-2016/03/31 |
