NBDC Research ID: hum0006.v1

SUMMARY

Aims: The identification of genomic/genetic alterations in brain tumors (gliomas) and analysis of their clinicopathological significance.

Methods: Exome sequence analysis using next generation sequencer (Illumina HiSeq 2000).

Participants/Materials: Surgical specimen of brain tumors (gliomas) and parid normal peripheral blood cells (Total: 23 samples).

Dataset ID	Type of Data	Criteria	Release Date
JGAS000004	NGS (Exome)	Controlled-access (Type I)	2014/01/31

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MOLECULAR DATA


Participants/Materials	6 brain tumors (gliomas)
Targets	Exome
Target Loci for Capture Methods	-
Platform	Illumina [HiSeq 2000]
Library Source	DNAs extracted from surgical specimen of brain tumors (gliomas) and parid normal peripheral blood cells.
Cell Lines	-
Library Construction (kit name)	Agilent SureSelect Human All Exon v.4
Fragmentation Methods	Covaris
Spot Type	Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	100 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000004
Total Data Volume	376 GB
Comments (Policies)	NBDC policy

Principal Investigator: Nobuhito Saito

Affiliation: Department of Neurosurgery, The University of Tokyo

Project / Group Name: Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT)

URL: http://p-direct.jfcr.or.jp/english/

Funds / Grants (Research Project Number):

Name	Title	Project Number
Ministry of Education, Culture, Sports, Science and Technology Japan (MEXT) P-DIRECT	Development of novel therapeutic strategy and biomarker for the treatment of malignant brain tumors	-

Name

Title

Project Number

Ministry of Education, Culture, Sports, Science and Technology Japan (MEXT)

P-DIRECT

Development of novel therapeutic strategy and biomarker for the treatment of malignant brain tumors

	Title	DOI	Dataset ID
1	Mutational Analysis Reveals the Origin and Therapy-Driven Evolution of Recurrent Glioma	doi:10.1126/science.1239947	JGAD000004
2

Principal Investigator:	Affiliation:	Data in Use (Dataset ID)	Period of Data Use