NBDC Research ID: hum0427.v1
SUMMARY
Aims: In the field of oncology, many researchers generally use cell lines obtained from cell banks. However, cell lines for head and neck malignancies available from cell banks are limited especially for rare-type cancers, resulting in difficulties in elucidation of biological characterization of such malignancies and establishment of therapeutic strategies. This study aimed to establish cell lines of head and neck malignancies and to examine their tumorigenic abilities in immune-deficient animals. The established cell lines will be deposited to cell banks.
Methods: Genomic DNAs extracted from peripheral blood mononuclear cells (PBMC), the primary tumor tissues, and the cell line were used for whole exome sequencing analysis.
Participants/Materials: PBMC and the primary tumor tissues were collected from a patient with external auditory canal squamous cell carcinoma, and a cell line was established from the primary tumor tissues. The cell line was deposited as SCEACono2 in RIKEN BioResource Research Center (https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB5515&lang=En).
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000645 | NGS (Exome) | Controlled-access (Type I) | 2023/11/02 |
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MOLECULAR DATA
| Participants/Materials |
external auditory canal squamous cell carcinoma (ICD10: C44.2): 1 case (3 samples) non-tumor tissue (PBMC), primary tumor tissue, cell line established from the primary tumor tissue: 1 sample each |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | MGI [DNBSEQ-G400] |
| Library Source | DNAs extracted from PBMC, the primary tumor tissues, and the cell line |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon V6 Kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000775 |
| Total Data Volume | 29.6 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Takashi Nakagawa
Affiliation: Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (B) | Development of novel synthesizing treatment strategy by exhaustive research on temporal bone squamous cell carcinoma | 18H02951 |
| KAKENHI Grant-in-Aid for Scientific Research (B) | Elucidation of epigenetic tumor control mechanisms that determine the malignancy of squamous cell carcinoma of the temporal bone | 22H03236 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma | doi: 10.1002/2211-5463.13225 | JGAD000775 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
