NBDC Research ID: hum0416.v1

Click to Latest version.

 

SUMMARY

Aims: In order to grasp the biological reactions and pathological conditions occurring in the body in more detail for diseases involving immune reactions, blood and tissue samples were used and analyzed by following the changes and changes over time for each patient. The present research will acquire and profile comprehensive parameters for each patient and explore factors related to disease conditions and treatments. Then, it will be linked to the understanding of pathology and the development of therapeutic methods through the confirmation of changes in the found molecules and the search for functions.

Methods: single-cell CITE-seq (Cellular indexing of transcriptomes and epitopes)

Participants/Materials: Peripheral blood mononuclear cells (PBMCs) were obtained from blood samples of 8 patients with microscopic polyangiitis (MPA) and 7 healthy donors.

 

Dataset IDType of DataCriteriaRelease Date
E-GEAD-635 NGS (CITE-seq) Unrestricted-access 2023/08/10

*Release Note

*When the research results including the data which were downloaded from NHA/DRA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more

 

MOLECULAR DATA

E-GEAD-635

Participants/Materials

microscopic polyangiitis (ICD10: M31.7): 8 cases

    blood samples were collected from 3 patients twice and 5 patients once

7 healthy donors

Targets CITE-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2500, NovaSeq 6000]
Library Source RNAs extracted from a single cell isolated from PBMCs
Cell Lines -
Library Construction (kit name) Chromium Single Cell 5' Reagent Kits v2
Fragmentation Methods Enzymatic fragmentation
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 91 bp
Software CellRanger (version 6.0.6)
Reference Genome Sequence GRCh38
QC Methods Cell Ranger Metrics
Genomic Expression Archive ID E-GEAD-635
Total Data Volume 311 MB (expression matrix, HDF5)
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Takehisa Kitazawa

Affiliation: Research Division, Chugai Pharmaceutical Co., Ltd.

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number

 

PUBLICATIONS

TitleDOIDataset ID
1 Single-cell multi-omics analysis identifies two distinct phenotypes of newly-onset microscopic polyangiitis doi: 10.1038/s41467-023-41328-0 E-GEAD-635
2