NBDC Research ID: hum0328.v1
SUMMARY
Aims: To examine the frequency of germline variants in DNA repair-associated genes among advanced Japanese prostate cancer patients
Methods: Leukocyte DNA from patients with metastatic prostate cancer at diagnosis or those who developed castration resistance during treatment were collected from multiple institutions in Japan. Multiple PCR-based target sequences of entire exon regions of the 27 genes were conducted.
Participants/Materials: 129 prostate cancer patients who were with metastasis at initial diagnosis or who developed castration-resistant prostate cancer during treatment and accepted data publication
Dataset ID | Type of Data | Criteria | Release Date |
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JGAS000509 | NGS (Target Capture) | Controlled-access (Type I) | 2023/05/11 |
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Participants/Materials | prostate cancer (ICD10: C61) : 129 cases |
Targets | Target Capture |
Target Loci for Capture Methods | 27 DNA repair-associated genes (APC, ATM, BARD1, BMPR1A, BRCA1, BRCA2, BRIP1, CDH1, CDK4, CDKN2A, CHEK2, EPCAM, HOXB13, MLH1, MSH2, MSH6, MUTYH, NBN, NF1, PALB2, PMS2, PTEN, RAD51C, RAD51D, SMAD4, STK11, TP53) |
Platform | Illumina [HiSeq2500] |
Library Source | DNAs extracted from peripheral blood cells |
Cell Lines | - |
Library Construction (kit name) |
multiplex PCR: 2xPlatinum Multiplex PCR Master Mix 2nd PCR: KAPA HiFi DNA Polymerase |
Fragmentation Methods | - |
Spot Type | Paired-end |
Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 150 bp |
Japanese Genotype-phenotype Archive Dataset ID | JGAD000627 |
Total Data Volume | 7.3 GB (fastq) |
Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Shusuke Akamatsu
Affiliation: Department of Urology, Graduate School of Medicine, Kyoto University
Project / Group Name: -
Funds / Grants (Research Project Number):
Name | Title | Project Number |
---|---|---|
KAKENHI Grant-in-Aid for Scientific Research (B) | Elucidating the biological effect of homologous recombination repair associated gene defect in prostate cancer using plasma cell-free DNA analysis | 20H03814 |
PUBLICATIONS
Title | DOI | Dataset ID | |
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1 | Prognostic significance of pathogenic variants in BRCA1, BRCA2, ATM and PALB2 genes in men undergoing hormonal therapy for advanced prostate cancer | doi: 10.1038/s41416-022-01915-2 | JGAD000627 |
2 |
USRES (Controlled-access Data)
Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
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