NBDC Research ID: hum0021.v3
SUMMARY
Aims: Identify genes relevant to bipolar disorder
Methods: Whole exome sequence analysis using Illumina HiSeq 2000/2500
Participants/Materials: Three pairs of healthy monozygotic twins, 66 trios with bipolar disorder proband and unaffected parents
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000014 | NGS (Exome) | Controlled-access (Type I) | 2015/01/26 |
| JGAS000273 | NGS (Exome) | Controlled-access (Type I) | 2021/03/05 |
| JGAS000273 (Data addition) | NGS (Exome) | Controlled-access (Type I) | 2021/04/20 |
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MOLECULAR DATA
| Participants/Materials | Three pairs of healthy monozygotic twins |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2000] |
| Library Source | gDNA extracted from peripheral blood cells |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon 50 Mb Kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris S220) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000014 |
| Total Data Volume | 118 GB |
| Comments (Policies) | NBDC policy |
This study was supported by “Genome Science”. “Genome Science” is a part of the “Support Programs for Three Fields in Life Sciences (cancer, genome and brain sciences)” established by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) under the Grant-in-Aid for Scientific Research on Innovative Areas.
| Participants/Materials | 66 + 78 trios with bipolar disorder proband (ICD10: F31) and unaffected parents |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2000/2500] |
| Library Source | gDNA extracted from saliva or peripheral blood cells |
| Cell Lines | - |
| Library Construction (kit name) |
SureSelect Human All Exon V5 (50 Mb) Kit SureSelect Human All Exon V6r2+lncRNA+mtDNA |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris S2) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 101 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000379 |
| Total Data Volume | 1.1 TB + 848.1 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Tadafumi Kato
Affiliation: RIKEN Brain Science Institute
Project / Group Name: Laboratory of Molecular Dynamics of Mental Disorders
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
|
KAKENHI Grant-in-Aid for Challenging Exploratory Research |
Search for genetic mutations by next generation sequencing of monozygotic twins discordant for bipolar disorder. |
21659277 |
| Platform Program for Promotion of Genome Medicine, Japan Agency for Medical Research and Development (AMED) | Integrated research for the realization of genome medicine of mental disorders | JP18km0405208 |
| Strategic Research Program for Brain Sciences, Japan Agency for Medical Research and Development (AMED) | Identification of genetically defined subsets of bipolar disorder by trio sequencing. | JP20dm0107133 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Exome sequencing for bipolar disorder points to roles of de novo loss-of-function and protein-altering mutations. | doi: 10.1038/mp.2016.69 | JGAD000379 |
| 2 | Systematic analysis of exonic germline and postzygotic de novo mutations in bipolar disorder | doi: 10.1038/s41467-021-23453-w | JGAD000379 |
USERS (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
| Hailiang Huang | The Broad Institute Stanley Center | Asian Bipolar Genetics Network (A-BIG-NET) |
JGAD000014 JGAD000379 |
2021/08/11-2022/03/02 | |
| Masahiro Nakatochi | Public Health Informatics Unit, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine | Japan | Research on elucidation of susceptibility to brain and mental diseases (vulnerability to disease onset) and efficacy and side effects of drugs (treatment responsiveness) through genetic analysis |
JGAD000379 |
2023/02/25-2025/10/28 |
