{"id":"hum0611","secondaryIds":[],"version":1,"url":"https://humandbs.dbcls.jp/research/hum0611/v1","datePublished":"2026-11-10","versions":[{"version":1,"datePublished":"2026-11-10"}],"title":{"ja":"造血器疾患患者血液細胞のタンパク質・遺伝子発現の解析","en":"Analysis of Protein and Gene Expression in Blood Cells from Patients with Hematologic Disorders"},"summary":{"aims":{"ja":"造血器疾患罹患者の血液細胞におけるタンパク質発現や遺伝子発現の解析を行い、特定の疾患における特徴的なタンパク質発現や遺伝子発現の変化を明らかにすること。","en":"To analyze protein and gene expression in blood cells obtained from patients with hematologic disorders in order to identify characteristic changes in protein and gene expression associated with specific diseases."},"methods":{"ja":"cDNAライブラリーは、SMART-Seq mRNA法（タカラバイオ）を用いて調製した。増幅された二本鎖cDNAは、AMPure XPビーズ（ベックマン・コールター）を用いて精製した。シーケンシングライブラリーは、Nextera XT DNA Library Prep Kit（イルミナ）およびIDT for Illumina DNA/RNA UDインデックスを用いて作成した。インデックス付きライブラリはPCRにより増幅され、品質管理が行われた。シーケンシングは、慢性GVHDの解析にはIllumina NovaSeq 6000プラットフォームで、フィセチンの解析にはIllumina NovaSeq X Plusプラットフォームを用いて実施され、ペアエンドリードが生成された。","en":"cDNA libraries were prepared using the SMART-Seq mRNA method (Takara Bio). The amplified double-stranded cDNA was purified using AMPure XP beads (Beckman Coulter). Sequencing libraries were generated using the Nextera XT DNA Library Prep Kit (Illumina) with IDT for Illumina DNA/RNA UD Indexes. Indexed libraries were amplified by PCR and subjected to quality control. Sequencing was performed on the Illumina NovaSeq 6000 platform for the chronic GVHD analysis and on the Illumina NovaSeq X Plus platform for the fisetin analysis, to generate paired-end reads."},"targets":{"ja":"造血器疾患罹患者の血液細胞\n1. 慢性移植片対宿主病（Graft-versus-host disease：GVHD）の有無の解析\n慢性GVHD有3症例／慢性GVHD無3症例の末梢血、計12検体（CD4+ 6検体、CD8+ 6検体）\n2. in vitro Fisetin投与の有無の解析\n慢性GVHD 1症例の末梢血4検体（Fisetin投与有CD4+, CD8+、投与無CD4+, CD8+）\n※2.の対象者は1.に含まれない","en":"Blood cells obtained from patients with hematologic disorders\n1. Analysis according to the presence or absence of chronic GVHD (cGVHD)\ncGVHD+ 3 cases and cGVHD– 3 cases\nA total of 12 peripheral blood samples: six CD4+ T-cell and six CD8+ T-cell samples.\n\n2. Analysis according to in vitro fisetin treatment\ncGVHD+ 1 case\nfisetin-treated CD4+ and CD8+ T-cell,  untreated CD4+ and CD8+ T-cell samples.\nA total of 4 peripheral blood samples.\n\nThe participant included in analysis 2 was not included in analysis 1."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"慢性移植片対宿主病：4症例\n造血器疾患罹患者（対照者）：3症例\n（日本人）","en":"cGVHD: 4 cases\nhematologic disorders (controls): 3 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"造血器疾患罹患者のうち、慢性移植片対宿主病（Graft-versus-host disease：GVHD）3症例および慢性GVHDの無い3症例の末梢血より分離したCD4+T細胞およびCD8+T細胞から抽出したRNAを用いたRNA sequencing 解析データをfastqファイルにて提供する。また、慢性GVHD 1症例のin vitro Fisetin投与有／投与無の末梢血より分離したCD4+T細胞およびCD8+T細胞から抽出したRNAを用いたRNA sequencing 解析データをfastqファイルにて提供する。","en":"RNAs extracted from CD4+ T-cell and CD8+ T-cell samples obtained from peripheral blood of cGVHD+ 3 cases and cGVHD– 3 individuals with hematologic disorders were used for the RNA sequencing analysis. RNAs extracted from CD4+ T-cell and CD8+ T-cell samples obtained from peripheral blood of  cGVHD+ 1 case with or without in vitro fisetin treatment were also used for the RNA sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"神保 光児","en":"Koji Jimbo"},"organization":{"name":{"ja":"東京大学医科学研究所 造血病態制御学分野","en":"Division of Hematopoietic Disease Control, The Institute of Medical Science, The University of Tokyo"}}}],"researchProjects":[],"grants":[{"title":{"ja":"造血細胞移植後のT細胞老化機構の解明と慢性移植片対宿主病への影響","en":"Mechanism of T cell senescence after hematopoietic cell transplantation and its impact on chronic graft-versus-host disease"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"KAKENHI Grant-in-Aid for Early-Career Scientists"},"grantIds":["22K16317"]}],"relatedPublications":[],"datasets":["JGAD001144"],"controlledAccessUsers":[]}