{"id":"hum0553","version":1,"url":"https://humandbs.dbcls.jp/research/hum0553/v1","datePublished":"2026-10-14","versions":[{"version":1,"datePublished":"2026-10-14"}],"title":{"ja":"ヒト消化器がん検体を用いた悪性化に関わる薬剤標的因子の探索研究","en":"Study of anti-malignant cancer drug target using human CRC patient samples"},"summary":{"aims":{"ja":"大腸がんや胃がんの死亡率は減少傾向にあるものの、転移を伴うがんの予後は悪い。がん悪性化の機構解明のため、ステージの異なる試料から腫瘍細胞および非腫瘍細胞を単離して培養し、これらの遺伝子解析を行う。遺伝子解析から得られた結果をもとに薬剤スクリーニングを行い、標的遺伝子と薬剤の効果を詳細に検証する。また、これらの結果をもとに免疫不全マウスに腫瘍細胞を移植後、形成された腫瘍に対する薬剤効果を個体モデルで検証する。","en":"Gastrointestinal cancer is the leading cause of cancer-related death, and the 5-year survival rate drops significantly for patients with metastasis. To study the mechanisms of malignant progression, tumor samples are collected from patients at different stages and organoids are established. Using these organoids, anti-cancer drug targets are identified by gene expression analysis, or PDX model analysis, drug screening analysis."},"methods":{"ja":"再発を繰り返す大腸がん患者の肝転移手術摘出検体から樹立した腫瘍オルガノイドを用いて薬剤感受性スクリーニングを行い、有効である薬剤の探索を行った。その結果に基づき、有効であったEGFR阻害薬、KRAS阻害薬、MEK阻害薬のコンビネーション治療実験を行い、シナジー効果を得られるか、RNA-seq解析ならびにExome-seq解析にて検証した。","en":"Whole exome sequencing, RNA sequencing"},"targets":{"ja":"大腸がん 1症例","en":"A colorectal cancer patient who underwent surgical resection of liver metastasis."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing,\nExpression profiling"},"targets":{"ja":"大腸癌：1症例\n（日本人）","en":"colorectal cancer: 1 case\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq）","en":"NGS\n(Exome, RNA-seq)"}},"releaseNote":{"ja":"大腸がん1症例の、肝臓転移腫瘍組織から樹立したオルガノイド4検体より抽出したDNAを用いたwhole exome sequencing、ならびに、オルガノイド6検体より抽出したRNAを用いたRNA sequencing解析データをfastqファイルにて提供する。","en":"DNA/RNA extracted from patient-derived organoids from liver metastases of a colorectal cancer patient were used for the whole exome sequencing and RNA sequencing analyses. Fastq files are provided."},"dataProviders":[{"name":{"ja":"大島 正伸","en":"Masanobu Oshima"},"organization":{"name":{"ja":"金沢大学 ナノ生命科学研究所","en":"Nano Life Science Institute, Kanazawa University"}}}],"researchProjects":[{"name":{"ja":"ヒト消化器がん検体を用いた悪性化に関わる薬剤標的因子の探索研究","en":null},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"外因性リガンド依存的Wntシグナル活性化による胃がん悪性化転移機構の研究","en":"Mechanisms of Gastric cancer Progression and Metastasis Driven by Exogenous Ligand-dependent Wnt Signaling"},"agency":{"ja":"科学研究費助成事業 基盤研究（A）","en":"KAKENHI Grant-in-Aid for Scientific Research (A)"},"grantIds":["22H00454"]},{"title":{"ja":"変異p53タンパク質蓄積不均一性に起因した腫瘍内局所微小環境に関する研究","en":"Intratumoral Microenvironment Associated with Heterogenous accumulation of Mutant p53"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["23K06631"]}],"relatedPublications":[],"datasets":["JGAD001019"],"controlledAccessUsers":[]}