{"id":"hum0544","version":1,"url":"https://humandbs.dbcls.jp/research/hum0544/v1","datePublished":"2026-03-10","versions":[{"version":1,"datePublished":"2026-03-10"}],"title":{"ja":"ヒト精子形成の研究とiPS細胞の樹立","en":"Research on human spermatogenesis and establishment of iPS cells"},"summary":{"aims":{"ja":"精巣は最も進化速度が速い組織として知られており、種間でその性質が大きく異なる。精子幹細胞は成体の精巣において精子形成に働く幹細胞である。これまでの研究により、霊長類の精子幹細胞系がマウスのものとは大きく違うことが明らかになった。例えば、マウスにおいては、精子幹細胞は少なくとも7日に一回は分裂するのに対して、サルでは約50日に一回程度しか分裂を行わない。このサルの精子幹細胞の性質は、寿命の長いサルにおいてDNA複製に伴う突然変異を少なくするために進化したと考えられる。ヒトの精子幹細胞もサルのものと同様の性質を持つと考えられるが、ヒトでは精子幹細胞のみならず精子形成自体の研究が進んでおらず良くわかっていない。本研究は、その機構の解明と不妊の原因究明を目的とする。","en":"The testis is known to be the tissue with the fastest evolutionary rate, and its properties vary greatly between species. Spermatogonial stem cells (SSCs) are the stem cells responsible for spermatogenesis in the adult testis. Previous research using monkeys has revealed that the SSC system in primates differs significantly from that in mice. This study aims to understand the primate SSC system and investigate the causes of human male infertility."},"methods":{"ja":"精巣をコラゲナーゼ4とトリプシンで分散し、FACSにて分離したSSEA4陽性の細胞集団から抽出したDNAを用いたsingle cell bisulfite-seq解析","en":"Testes were dispersed with collagenase 4 and trypsin. SSEA4-positive cells were collected by FACS and used for single-cell bisulfite-seq analysis."},"targets":{"ja":"閉塞性無精子症","en":"Normal testes from obstructive azoospermia"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"メチル化","en":"Methylation profiling"},"targets":{"ja":"閉塞性無精子症：1症例\n（日本人）","en":"obstructive azoospermia: 1 case\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scWGBS）","en":"NGS\n(scWGBS)"}},"releaseNote":{"ja":"閉塞性無精子症の正常精巣から抽出したDNAを用いたsingle cell bisulfite-seq解析結果をfastqファイルにて提供する。","en":"DNAs extracted from SSEA4-positive cells isolated from normal testes obtained from a patient with obstructive azoospermia were used for the single-cell bisulfite-seq analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"渡部 聡朗","en":"Toshiaki Watanabe"},"organization":{"name":{"ja":"国立成育医療研究センター 細胞医療研究部","en":"Center for Regenerative Medicine, National Center for Child Health and Development"}}}],"researchProjects":[{"name":{"ja":"霊長類・ヒト生殖細胞プロジェクト/渡部グループ","en":"Primate/human germ cell research project/Watanabe group"},"url":{"ja":[{"url":"https://www.amed.go.jp/content/000153272.pdf","text":"https://www.amed.go.jp/content/000153272.pdf"}],"en":[{"url":"https://www.amed.go.jp/content/000153312.pdf","text":"https://www.amed.go.jp/content/000153312.pdf"}]}}],"grants":[{"title":{"ja":"霊長類生殖細胞形成におけるDNAメチル化の確立に関する研究","en":"Study of the establishment of DNA methylation during primate germ cell development"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的先端研究開発支援事業 ソロタイプ（PRIME）","en":"Precursory Research for Innovative Medical care (PRIME), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP22gm6310010"]}],"relatedPublications":[{"title":"Establishment of DNA methylation during primate germ cell development","doi":"https://doi.org/10.1038/s41467-026-71405-z","datasets":["JGAD001032","JGAD001031"]}],"datasets":["JGAD001032"],"controlledAccessUsers":[]}