{"id":"hum0542","version":1,"url":"https://humandbs.dbcls.jp/research/hum0542/v1","datePublished":"2026-03-09","versions":[{"version":1,"datePublished":"2026-03-09"}],"title":{"ja":"生殖細胞DNAメチル化確立に関する研究","en":"Studies on establishment of germline DNA methylation"},"summary":{"aims":{"ja":"これまで、サルを用いた研究により霊長類生殖細胞におけるDNAメチル化確立時期やそのメカニズムを明らかにしてきた。これらの成果を活用し、ヒトの精巣・卵巣の生殖細胞のDNAメチル化確立について理解することを目的とする。本研究により、ヒトの生殖細胞発生の理解が進み、配偶子DNAメチル化異常の予防や不妊治療法の開発に貢献する。","en":"Previous studies using monkeys have revealed the timing and mechanism of DNA methylation establishment in primate germ cells. The purpose of this study is to understand the establishment of DNA methylation in human testicular and ovarian germ cells. This study will advance our understanding of human germ cell development and contribute to the prevention of abnormal gamete DNA methylation and the development of infertility treatments."},"methods":{"ja":"single cell Whole Genome Bisulfite-sequence法","en":"single-cell whole genome bisulfite-sequencing"},"targets":{"ja":"外科手術を受けた10歳未満の精巣腫瘍症例","en":"Three testicular cancer patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"メチル化","en":"Methylation profiling"},"targets":{"ja":"精巣腫瘍：3症例\n（日本人）","en":"testicular teratoma: 3 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scWGBS）","en":"NGS\n(scWGBS)"}},"releaseNote":{"ja":"精巣腫瘍の外科手術を受けた10歳未満の3症例から摘出された精巣腫瘍周辺の正常精巣3検体（1検体につき10-20細胞程度）から抽出したDNAを用いたsingle cell Whole Genome Bisulfite-sequence解析結果をfastqファイルにて提供する。","en":"DNAs extracted from germ cells isolated from normal testes in teratomas obtained from three testicular cancer patients were used for the single cell Whole Genome Bisulfite-sequence analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"渡部 聡朗","en":"Toshiaki Watanabe"},"organization":{"name":{"ja":"国立成育医療研究センター 細胞医療研究部","en":"Center for Regenerative Medicine, National Center for Child Health and Development"}}}],"researchProjects":[{"name":{"ja":"霊長類・ヒト生殖細胞プロジェクト/渡部グループ","en":"Primate/human germ cell research project/Watanabe group"},"url":{"ja":[{"url":"https://www.amed.go.jp/content/000153272.pdf","text":"https://www.amed.go.jp/content/000153272.pdf"}],"en":[{"url":"https://www.amed.go.jp/content/000153312.pdf","text":"https://www.amed.go.jp/content/000153312.pdf"}]}}],"grants":[{"title":{"ja":"霊長類生殖細胞形成におけるDNAメチル化の確立に関する研究","en":"Study of the establishment of DNA methylation during primate germ cell development"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的先端研究開発支援事業 ソロタイプ（PRIME）","en":"Precursory Research for Innovative Medical care (PRIME), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP22gm6310010"]}],"relatedPublications":[{"title":"Establishment of DNA methylation during primate germ cell development","doi":"https://doi.org/10.1038/s41467-026-71405-z","datasets":["JGAD001031","JGAD001032"]}],"datasets":["JGAD001031"],"controlledAccessUsers":[]}