{"id":"hum0521","version":1,"url":"https://humandbs.dbcls.jp/research/hum0521/v1","datePublished":"2025-08-29","versions":[{"version":1,"datePublished":"2025-08-29"}],"title":{"ja":"がん免疫の分子機構解明","en":"Elucidation of Molecular Mechanisms of Cancer Immunity"},"summary":{"aims":{"ja":"子宮体がんの腫瘍微小環境におけるB細胞・T細胞の特徴の理解を深めることで、子宮体がんにおける腫瘍免疫を明らかにする。","en":"This study aims to clarify tumor immunity in uterine cancer. In particular, we aim to describe the characteristics of B cells and T cells in the tumor microenvironment of uterine cancer and to deepen our understanding of them."},"methods":{"ja":"子宮組織検体を酵素処理後、MACS法（Magnetic cell sorting：MACS）によりCD45陽性リンパ球を分離した。次にFACS法（Fluorescence-activated cell sorting）にてCD19陽性B細胞およびCD3陽性T細胞を分離した。分離したB細胞およびT細胞を対象としたsingle-cell（sc）RNA-seq、scBCR-seq、scTCR-seq、ならびにCITE-seq解析を実施した。","en":"Uterine specimens were digested with enzymes to separate CD45+ lymphocytes using MACS (Magnetic cell sorting). Subsequently, CD19+ B cells and CD3+ T cells were separated using FACS (Fluorescence-activated cell sorting). Isolated B and T cells were then analyzed using single-cell RNA-seq, single-cell BCR-seq, single-cell TCR-seq, and CITE-seq."},"targets":{"ja":"子宮体がん10症例の腫瘍組織および遺伝性乳がん卵巣がん症候群1症例の正常子宮組織","en":"tumor tissues of 10 uterine cancer patients, normal uterine tissue from 1 patient with hereditary breast and ovarian cancer syndrome"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"子宮体癌：10症例\n遺伝性乳癌卵巣癌：1症例\n（日本人）","en":"uterine cancer: 10 cases\nhereditary breast and ovarian cancer syndrome: 1 case\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq、scBCR-seq、scTCR-seq、CITE-seq）","en":"NGS\n(scRNA-seq, scBCR-seq, scTCR-seq, CITE-seq)"}},"releaseNote":{"ja":"子宮体がん10症例の腫瘍組織、および、遺伝性乳がん卵巣がん症候群1症例の正常子宮組織より分離したB細胞およびT細胞から抽出したRNAを用いたscRNA-seq、scBCR-seq、scTCR-seq、CITE-seqデータをfastq、h5、csv、tsvファイル形式で提供する。","en":"B and T cells isolated from tumor tissues of 10 uterine cancer patients and normal uterine tissue from 1 patient with hereditary breast and ovarian cancer syndrome were used for scRNA-seq, scBCR-seq, scTCR-seq and CITE-seq analyses. Fastq, h5, csv and tsv files are provided."},"dataProviders":[{"name":{"ja":"上野 英樹","en":"Hideki Ueno"},"organization":{"name":{"ja":"京都大学医学系研究科 免疫細胞生物学","en":"Department of Immunology, Graduate School of Medicine, Kyoto University"}}}],"researchProjects":[],"grants":[],"relatedPublications":[{"title":"Oligoclonal expansion of IgG+ B cells along with Tfh cell response is associated with a better outcome in endometrial cancer","doi":"https://doi.org/10.1093/intimm/dxaf049","datasets":["JGAD000969","E-GEAD-1121"]}],"datasets":["JGAD000969","E-GEAD-1121"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000969"]}]}