{"id":"hum0511","version":1,"url":"https://humandbs.dbcls.jp/research/hum0511/v1","datePublished":"2026-07-30","versions":[{"version":1,"datePublished":"2026-07-30"},{"version":2,"datePublished":"2026-09-18"}],"title":{"ja":"百寿者サンプルを用いた老化における分子マーカーの探索と分子基盤の解明","en":"Identification of Molecular Markers and Elucidation of Molecular Mechanisms in Aging Using Centenarian Samples"},"summary":{"aims":{"ja":"百寿者、百寿者の家族、及び対照群について、血液中に含まれるRNA全体および1細胞毎のRNAを解析し、老化に伴って増加・減少する分子マーカー、ならびに百寿者に特異的に存在するRNA分子の同定を行う。加えて、免疫の司令塔であるT細胞が老化によって変化する分子メカニズムを明らかにする。","en":"The purpose of this study is to analyze the total RNA in blood and single-cell RNA from centenarians, their family members, and control groups, with the aim of identifying molecular markers that increase or decrease with aging, as well as RNA molecules specific to centenarians. Additionally, the study seeks to elucidate the molecular mechanisms by which T cells, the central regulators of the immune system, change during aging."},"methods":{"ja":"scRNA-seq解析、16種類の細胞表面蛋白質発現（scCITE-seq）解析、TCRα/β鎖CDR3領域の配列（シングルセルレパトア）解析","en":"scRNA-seq, Expression data of 16 cell surface proteins (scCITE-seq), CDR3 sequences of T-cell receptor alpha and beta chains (scTCR-seq)"},"targets":{"ja":"データセット1：健常者28名（70～90歳代：8名、100歳代：10名、110歳代：10名）のT細胞\nデータセット2：上記のうち6名（70～90歳代：2名、100歳代：2名、110歳代：2名）のT細胞（Phorbol 12-myristate 13-acetate [PMA]とイオノマイシンで6時間刺激）","en":"Dataset 1: T cells from 28 healthy individuals\nDataset 2: T cells from 6 healthy individuals stimulated for 6 hours with Phorbol 12-myristate 13-acetate (PMA) and ionomycin"},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"健常者28名の末梢血T細胞から抽出したRNAを用いたscRNA-seq、scTCR-seq、scCITE-seqデータをfastq、tsv、mtx、txtファイル形式で提供する。","en":"RNAs extracted from peripheral blood T cells isolated from 28 healthy individuals were used for scRNA-seq, scTCR-seq and scCITE-seq analyses. Fastq, tsv, mtx and txt files are provided."},"dataProviders":[{"name":{"ja":"橋本 浩介","en":"Kosuke Hashimoto"},"organization":{"name":{"ja":"大阪大学 蛋白質研究所","en":"Institute for Protein Research, The University of Osaka"}}}],"researchProjects":[],"grants":[{"title":{"ja":"老化後期におけるCD4キラーT細胞の増加プロセスの解明","en":"Elucidation of the Expansion Mechanism of Cytotoxic CD4 T Cells During Late-Stage Aging"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["21K06135"]},{"title":{"ja":"長寿者のT細胞が保有する抗原記憶の解明","en":"Elucidation of Antigenic Memory in T Cells from Long-Lived Individuals"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["24K09423"]}],"relatedPublications":[{"title":"CD4 CTLs in Supercentenarians: Signs of Adaptive Expansion in Healthy Aging","doi":"https://doi.org/10.1016/j.celrep.2026.117728","datasets":["JGAD000957","E-GEAD-1107","E-GEAD-1108"]}],"datasets":["JGAD000957","E-GEAD-1107","E-GEAD-1108"],"controlledAccessUsers":[]}