{"id":"hum0497","version":1,"url":"https://humandbs.dbcls.jp/research/hum0497/v1","datePublished":"2025-08-06","versions":[{"version":1,"datePublished":"2025-08-06"}],"title":{"ja":"ヒト胎盤からの栄養膜幹細胞の単離と性状解析","en":"Isolation and characterization of trophoblast stem cells from human placenta"},"summary":{"aims":{"ja":"ヒト妊娠後期胎盤から栄養膜幹細胞を樹立することで、妊娠後期に発症の多い妊娠高血圧症候群などの胎盤関連妊娠合併症の病態解明に貢献する。","en":"Establishing trophoblast stem cells from the human term placenta contributes to elucidating the pathophysiology of placenta-related pregnancy complications, such as preeclampsia, which commonly occur in late pregnancy."},"methods":{"ja":"RNA sequence解析","en":"RNA sequencing"},"targets":{"ja":"3名のヒト妊娠後期胎盤の絨毛膜から樹立した栄養膜幹細胞（Ch-TS）と、それを分化誘導した絨毛外栄養膜細胞（Ch-EVT）ならびに合胞体栄養膜細胞（Ch-ST）（計9検体）、および、RIKEN BRCより入手した栄養膜幹細胞（CT27、CT29、bTS5）と、それらを分化誘導した絨毛外栄養膜細胞ならびに合胞体栄養膜細胞（計7検体）","en":"Three trophoblast stem cell (Ch-TS) lines derived from the chorion of term placentas from three individuals, as well as differentiated extravillous trophoblast cells (Ch-EVT) and syncytiotrophoblast cells (Ch-ST). Additionally, three trophoblast stem cell lines obtained from RIKEN BRC (CT27, CT29, bTS5) and their differentiated extravillous trophoblast cells and syncytiotrophoblast cells."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"ヒト妊娠後期胎盤：3名\n（日本人）\nヒト栄養膜幹細胞：3検体\n（細胞株）","en":"term placentas from three individuals\n(Japanese)\nthree trophoblast stem cell lines\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"3名のヒト妊娠後期胎盤の絨毛膜から樹立した栄養膜幹細胞と、それを分化誘導した絨毛外栄養膜細胞ならびに合胞体栄養膜細胞、および、RIKEN BRCより入手した栄養膜幹細胞と、それらを分化誘導した絨毛外栄養膜細胞ならびに合胞体栄養膜細胞から抽出したRNAを用いたRNA-seq解析データをfastqおよびcsvファイルにて提供する。","en":"RNAs extracted from trophoblast stem cell lines derived from the chorion of term placentas from three individuals and their differentiated extravillous trophoblast cells and syncytiotrophoblast cells, as well as trophoblast stem cell lines obtained from RIKEN BRC and their differentiated extravillous trophoblast cells and syncytiotrophoblast cells were used for RNA-sequencing analysis. Fastq and csv files are provided."},"dataProviders":[{"name":{"ja":"依馬 正次","en":"Masatsugu Ema"},"organization":{"name":{"ja":"滋賀医科大学 動物生命科学研究センター 幹細胞・ヒト疾患モデル研究分野","en":"Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science"}}}],"researchProjects":[],"grants":[{"title":{"ja":"妊娠高血圧症候群患者由来の栄養膜幹細胞の樹立と病態モデルの構築","en":"Establishment of trophoblast stem cells derived from patients with hypertensive disorders of pregnancy"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["22K09639"]},{"title":{"ja":"非ヒト霊長類の着床、胎盤形成機構の解明とヒトへの展開","en":"Elucidation of implantation and placental development mechanisms in non-human primates and their application to human biology"},"agency":{"ja":"科学研究費助成事業 挑戦的研究（開拓）","en":"KAKENHI Grant-in-Aid for Challenging Research (Pioneering)"},"grantIds":["24K21265"]},{"title":{"ja":"霊長類の内部細胞塊ダイナミズムと全能性","en":"Inner cell mass dynamism and totipotency in primate"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["22H04669"]},{"title":{"ja":"非ヒト霊長類の胚盤胞における内部細胞塊と栄養膜細胞の分化運命の解明","en":"Origin of placental tissues in cynomolgus monkey"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["20H05359"]},{"title":{"ja":"妊娠高血圧症候群患者由来の栄養膜幹細胞の樹立と病態モデルの構築","en":"Generation of Trophoblast Stem Cell from Preeclampsia"},"agency":{"ja":"今井精一記念財団","en":"The Seiichi Imai Memorial Foundation"},"grantIds":null}],"relatedPublications":[{"title":"Establishment of Human Trophoblast Stem Cells from Term Smooth Chorion","doi":"https://doi.org/10.1016/j.placenta.2025.07.090","datasets":["JGAD000919"]}],"datasets":["JGAD000919"],"controlledAccessUsers":[]}