{"id":"hum0491","version":1,"url":"https://humandbs.dbcls.jp/research/hum0491/v1","datePublished":"2025-07-04","versions":[{"version":1,"datePublished":"2025-07-04"}],"title":{"ja":"アレルギー病態の分子機構解明","en":"Elucidating molecular mechanisms of allergy"},"summary":{"aims":{"ja":"二次リンパ組織における胚中心反応は高親和性抗体を産生するための反応であり、主に CD4+ T細胞によって制御されている。しかしヒトにおいて二次リンパ組織の CD4+ T 細胞の生態は十分に描写されていない。特に胚中心反応を抑制するIL10+ Foxp3− 濾胞CD4+ T（T follicular regulatory type 1, Tfr1）細胞の分化・局在・多様性は未解明である。本研究はヒト二次リンパ組織におけるCD4+ T細胞を詳細に描写し、IL10+ Foxp3− 濾胞CD4+ T細胞の分化・局在・多様性を解明することを目的とする。","en":"Germinal center reactions in secondary lymphoid tissues are those responsible for the production of high-affinity antibodies and are primarily regulated by CD4+ T cells. However, the biology of CD4+ T cells in secondary lymphoid tissues has not been well described in humans. In particular, the differentiation, localization, and heterogeneity of IL10+ Foxp3- follicular CD4+ T cells (T follicular regulatory type 1, Tfr1 cells), which suppress the germinal center response, remain unresolved. This study aims to describe CD4+ T cells in human secondary lymphoid tissues in detail and to elucidate the differentiation, localization and heterogeneity of IL10+ Foxp3- follicular CD4+ T cells."},"methods":{"ja":"扁桃検体からFACSでCD4+T細胞（whole または Tfr1-enriched）を分離し、1細胞マルチオミクス解析（scRNA-seq、scRNA-TCR-seq、scATAC-RNA-seq）を行った。なお、一部の実験では CITE-seq を併用した。","en":"CD4+ T cells (whole or Tfr1-enriched) were isolated from tonsil specimens by FACS and subjected to the following single-cell multi-omics analyses: scRNA-seq, scRNA-TCR-seq, scATAC-RNA-seq. In some experiments, CITE-seq was combined."},"targets":{"ja":"慢性扁桃炎8症例の扁桃組織","en":"Chronic tonsillitis 8 cases"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\nクロマチン構造","en":"Expression profiling and chromatin structure analysis"},"targets":{"ja":"慢性扁桃炎：8症例\n（日本人）","en":"Chronic tonsillitis: 8 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq、scRNA-TCR-seq、scATAC-RNA-seq、CITE-seq）","en":"NGS\n(scRNA-seq, scRNA-TCR-seq, scATAC-RNA-seq, CITE-seq)"}},"releaseNote":{"ja":"慢性扁桃炎8症例の扁桃組織より分離したCD4+T細胞を用いた scRNA-seq、scRNA-TCR-seq、scATAC-RNA-seq、CITE-seq 生データ（fastq）、CITE-seq 抗体バーコードデータ（csv）、リードカウントデータ （h5）、TCRクロノタイプデータ（csv）、オープンクロマチン領域データ（tsv.gz、tsv.gz.tbi）を提供する。","en":"CD4+ T cells isolated from 8 tonsil specimens by FACS were used for scRNA-seq, scRNA-TCR-seq, scATAC-RNA-seq, and CITE-seq analyses. Fastq files and CITE-seq antibody/barcode data (csv files) are provided. It also provides read count data for each analyses (h5), TCR repertoire clonotype data (csv), and open chromatin region data (tsv.gz and tsv.gz.tbi)."},"dataProviders":[{"name":{"ja":"上野 英樹","en":"Hideki Ueno"},"organization":{"name":{"ja":"京都大学医学系研究科 免疫細胞生物学","en":"Department of Immunology, Graduate School of Medicine, Kyoto University"}}}],"researchProjects":[],"grants":[],"relatedPublications":[{"title":"Single-cell multiomic analysis revealed the differentiation, localization, and heterogeneity of IL10+ Foxp3- follicular T cells in humans.","doi":"https://doi.org/10.1093/intimm/dxaf014","datasets":["JGAD000947","E-GEAD-1086"]}],"datasets":["JGAD000947","E-GEAD-1086"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000947"]}]}