{"id":"hum0477","version":2,"url":"https://humandbs.dbcls.jp/research/hum0477/v2","datePublished":"2025-06-02","versions":[{"version":1,"datePublished":"2024-10-16"},{"version":2,"datePublished":"2025-06-02"}],"title":{"ja":"消化管癌・消化管腫瘍の組織培養による癌発育・進展メカニズムに関する研究","en":"Tumor tissue culture and its characterization in gastrointestinal cancer"},"summary":{"aims":{"ja":"日本人の死亡原因の第一位はがんであり、いまだ増加傾向にある。近年、生体におけるがんはがん細胞のみならず線維芽細胞や免疫細胞など様々な細胞から構成される微小環境・がん間質ががんの増殖、浸潤、転移、治療抵抗性に深く関与していることが報告されている。オルガノイドは、器官特異的な立体構造や機能を再現でき、ヒトを対象とした疾患研究・生物医学研究・再生医療への活用に期待が集まっている。本研究は、消化管がんの治療成績の向上を目的とし、群馬大学医学部附属病院外科診療センターで治療を受けた消化管腫瘍およびその疑い患者から診断および治療上不都合のないように組織を採取し、オルガノイド培養やDish培養を行い、その特性およびがん細胞と微小環境の関連メカニズムについて解析し、消化管腫瘍における新たな治療を開発することを目指す。本研究により、消化管腫瘍の新たなメカニズムや新規治療標的の同定、新規治療の開発が期待される。","en":"Cancer is the leading cause of death in Japan and is continuing to rise. It has been reported that tumor microenvironment is linked to the tumor development, invasion, metastasis and residence of cancer to therapy. Organoid can reproduce its organ structure and function, there are expectations for their application in biomedical research, regenerative medicine, and human disease study. We collected tissues from patients with gastrointestinal tract or suspected tumors treated at Gunma University Hospital to avoid inconveniences in diagnosis and treatment. We perform organoid culture, analyzing their properties and mechanism related to cancer cells and microenvironment. This research is expected to identify mechanisms of tumor development, novel therapeutic targets and develop new therapies."},"methods":{"ja":"Bifidobacterium longum subsp. longum（B. longum：ビフィドバクテリウム ロンガム 亜種 ロンガム）と、腸管オルガノイド由来上皮細胞もしくはCaco-2細胞を24時間共培養後、あるいは、共培養前の上皮細胞とCaco-2細胞を対象にRNA-seq解析を行った。","en":"RNA-seq analysis of epithelial cells from human colon organoid and Caco-2 cells was conducted before and after co-cultivation of epithelial cells and Bifidobacterium longum subsp. longum (B. longum) for 24 hours."},"targets":{"ja":"大腸がん患者由来非腫瘍組織：B.longumとの共培養前3検体、共培養後 3＋3検体、B. longumなしで培養7検体\nCaco-2細胞：B.longumとの共培養前3検体、共培養後 3＋3検体、B. longumなしで培養7検体","en":"Healthy tissues from a colorectal cancer patient (before co-cultivation with B. longum 3 sample, after co-cultivation with B. longum 3 + 3 samples and cultivation without B. longum 7 sample)\nCaco-2 cells (before co-cultivation with B. longum 3 sample, after co-cultivation with B. longum 3 + 3 samples and cultivation without B. longum 7 sample)"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"大腸癌患者由来の腸管オルガノイド：1検体\n（日本人）\nCaco-2細胞：1検体\n（細胞株）","en":"Organoids from a colorectal cancer patient: 1 sample\n(Japanese)\nCaco-2 cell: 1 sample\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"大腸がん患者の腸管（非腫瘍組織）から作製したオルガノイド由来の上皮細胞もしくはCaco-2細胞について、Bifidobacterium longum subsp. longumとの24時間共培養後、および、B. longumなしでの24時間培養後の細胞より抽出したRNAを用いたRNA-seq解析データをfastqおよびcsvファイルにて提供する。","en":"RNAs extracted from epithelial cells from non-tumor colon organoid from a colorectal cancer patient and Caco-2 cells conducted after co-cultivation with Bifidobacterium longum subsp. longum (B. longum) or cultivation without B. longum were used for RNA-sequencing analysis. Fastq and csv files are provided."},"dataProviders":[{"name":{"ja":"佐々木 伸雄","en":"Nobuo Sasaki"},"organization":{"name":{"ja":"群馬大学 生体調節研究所 粘膜エコシステム制御分野","en":"Laboratory of Mucosal Ecosystem Design, Institute for Molecular and Cellular Regulation, Gunma University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"ヒューマン粘膜エコシステムを標的とした腸内細菌創薬プラットフォーム開発研究","en":"Development of Scalable Platform of the Drug Discovery Targeting for Human Mucosal Ecosystem"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代治療・診断実現のための創薬基盤技術開発事業","en":"Project Focused on Developing Key Technology for Discovering and Manufacturing Drugs for Next-Generation Treatment and Diagnosis, Japan Agency for Medical Research and Developmstent (AMED)"},"grantIds":["JP23ae0121046"]},{"title":{"ja":"組織幹細胞を制御する\"加菌\"システムの開発","en":"Development of a novel culturing system for gut bacteria to regulate adult tissue stem cells"},"agency":{"ja":"国立研究開発法人科学技術振興機構（JST） 創発的研究支援事業","en":"Fusion Oriented REsearch for disruptive Science and Technology (FOREST) program, Japan Science and Technology Agency (JST)"},"grantIds":["JPMJFR2161"]},{"title":{"ja":"次世代型オルガノイドライブラリー構築による腸内環境依存的な発癌機構の理解","en":"Generating the next generation of organoid biobank to reveal intestinal environment-dependent tumorigenesis"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["23H02713"]},{"title":{"ja":"ヒト腸内エコシステムin vitro再現系を利用した細菌変動予測モデルの構築","en":"Predictive modeling of bacteria variation using in vitro reconstructions of the human gut ecosystem"},"agency":{"ja":"科学研究費助成事業 挑戦的研究（開拓）","en":"KAKENHI Grant-in-Aid for Challenging Research (Pioneering)"},"grantIds":["23K17415"]}],"relatedPublications":[],"datasets":["JGAD000875"],"controlledAccessUsers":[]}