{"id":"hum0476","version":2,"url":"https://humandbs.dbcls.jp/research/hum0476/v2","datePublished":"2025-06-15","versions":[{"version":1,"datePublished":"2025-01-07"},{"version":2,"datePublished":"2025-06-15"}],"title":{"ja":"腎臓構成細胞の生物学的機能理解のための先端的解析技術を用いた観察研究","en":"Observational studies using advanced analytical techniques to understand the biological functions of kidney component cells"},"summary":{"aims":{"ja":"腎臓を構成する細胞の機能の理解や腎疾患の生物学的理解のため、腎臓構成細胞のシングルセルトランスクリプトーム解析や空間的トランスクリプトーム解析を行い、ヒト腎臓構成細胞の遺伝子発現プロファイルと臨床的パラメータとの関連解析を行う。また、腎疾患の理解のための医学研究や腎疾患の治療のための創薬研究に活用できる基盤を構築する。","en":"To establish a foundation for understanding the functions of kidney component cells and the biology of renal disease. Single cell and spatial transcriptome analyses of human kidney component cells will be performed to analyze the gene expression profiles of those cells and explore their relationship between the gene expression profiles and clinical parameters. The research foundation established here is worthwhile for understanding disease mechanisms in the kidney or discovering novel therapeutic drugs for renal disease."},"methods":{"ja":"シングルセルRNAシークエンス解析（Chromium）、空間的トランスクリプトーム解析（Visium）","en":"Single cell RNA sequencing analysis, Spatial transcriptome analysis (Visium)"},"targets":{"ja":"腎臓がん32症例の摘出腎の内、非腫瘍部","en":"Noncancerous tissue (including other findings) from surgically removed from 32 renal cancer patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\n病理画像","en":"Expression profiling; Histological imaging"},"targets":{"ja":"腎臓癌：32症例\n（日本人）","en":"32 renal cancer\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq、Visium Spatial Gene Expression）\n病理画像","en":"NGS (scRNA-seq, Visium Spatial Gene Expression); Histological image"}},"releaseNote":{"ja":"腎臓がん6症例（正常腎組織検体：3検体、IgA腎症の所見を呈する検体：3検体）のscRNA-seqのリードカウントデータ等をtsvおよびmtxファイル形式で提供する。","en":"Read count data of scRNA-seq for 6 renal cancer patients (3 samples of normal tissue and 3 samples of findings of IgA nephropathy) are provided (tsv and mtx files)."},"dataProviders":[{"name":{"ja":"内藤 正吉","en":"Shokichi Naito"},"organization":{"name":{"ja":"北里大学 医学部腎臓内科","en":"Nephrology, Kitasato University school of Medicine"}}}],"researchProjects":[],"grants":[],"relatedPublications":[{"title":"Inflammatory molecules in the glomerular endothelium in mild IgA-Nephropathy identified by single-cell and spatial transcriptome","doi":"https://doi.org/10.1101/2024.10.30.621002","datasets":["JGAD000871"]}],"datasets":["JGAD000871"],"controlledAccessUsers":[]}