{"id":"hum0469","version":1,"url":"https://humandbs.dbcls.jp/research/hum0469/v1","datePublished":"2024-08-14","versions":[{"version":1,"datePublished":"2024-08-14"}],"title":{"ja":"妊娠中のサイトメガロウイルス免疫応答の検討","en":"Investigation of immune response to cytomegalovirus during pregnancy"},"summary":{"aims":{"ja":"胎内サイトメガロウイルス（以下CMV）感染症は、乳幼児に神経学的後遺症を引き起こす最も頻度の高い周産期ウイルス感染症である。妊婦がCMVに初感染した場合、およそ40%の確立で胎盤を通過し、先天性CMV感染が発生する。現在の我国における先天性CMV感染の頻度は全出生児300人当り1人と他の先天性代謝異常よりも多く、その早期対策が望まれているが、未だワクチンは存在せず、妊娠中の感染を明らかにする検査方法・治療法も確立していない。我々は今回、CMV感染に際しての母体の免疫応答に着目し、CMV由来の抗原ペプチドに対して反応するB細胞受容体（以下BCR）やT細胞受容体（以下TCR）のクローナリティについて解析を行うことで、その機能や特徴を解明し、現在行われているCMV母子感染のスクリーニング検査方法の有用性を検討するほか、ワクチンや治療法の開発につなげていくことを目指す。","en":"In utero CMV infection is the most frequent perinatal viral infection causing neurological sequelae in infants. When a pregnant woman is first infected with CMV, approximately 40% of cases cross the placenta, resulting in congenital CMV infection. In this study, we focused on the maternal immune response to CMV infection and analyzed the clonality of B cell receptors (BCR) and T cell receptors (TCR) that react to CMV-derived antigen peptides to elucidate their functions and characteristics, and to investigate the usefulness of current screening methods for mother-to-child CMV infection. The aim is to examine the usefulness of current screening methods for mother-to-child transmission of CMV, and to develop vaccines and treatments."},"methods":{"ja":"末梢血単核球（PBMC）単離後、単一細胞RNA sequence解析を実施した。","en":"PBMCs were used for single-cell RNA sequence analysis."},"targets":{"ja":"東京大学医学部附属病院にて妊娠初期スクリーニング検査を受けた妊婦\n12週ごろ4検体、20週ごろ7検体、28週ごろ1検体、36週ごろ3検体 合計10名15検体","en":"Pregnant women undergoing early pregnancy screening (around 12 weeks' gestation) at the University of Tokyo Hospital\n4 specimens around 12 weeks, 7 specimens around 20 weeks, 1 specimen at 28 weeks, 3 specimens around 36 weeks"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"妊娠12週、16週、20週、28週、36週頃の妊婦：10名\n（日本人）","en":"Pregnant women undergoing early pregnancy screening: 10 individuals\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"妊娠12週、16週、20週、28週、36週頃の検診時採血の単核球（PBMC）から抗原特異的T細胞を単離後、抽出したRNAを用いたscRNA-seq解析データをfastqおよびcsvファイルにて提供する。","en":"RNAs extracted from PBMC of pregnant women undergoing pregnancy screening (12 weeks, 20 weeks, 28 weeks, or 36 weeks) were used for single-cell RNA-sequencing analysis. Fastq and csv files are provided."},"dataProviders":[{"name":{"ja":"市瀬 茉里","en":"Mari Ichinose"},"organization":{"name":{"ja":"東京大学 女性診療科・産科","en":"Obstetrics and Gynecology, The University of Tokyo"}}}],"researchProjects":[],"grants":[{"title":{"ja":"T細胞受容体の抗原エピトープを予測する機械学習システムの開発、検証、及び、応用","en":"Development, validation, and application of machine learning system for TCR epitope prediction"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["20K06610"]},{"title":{"ja":"抗原特異的T細胞プロファイリングによる、妊婦におけるサイトメガロ感染症の免疫動態の解明と診断法の開発","en":"Elucidation of immunokinetics and development of diagnostic modality for congenital cytomegalovirus infection by antigen-specific T cell profiling at single cell level"},"agency":{"ja":"公益財団法人 神澤医学研究振興財団","en":"Kanzawa Medical Research Foundation"},"grantIds":null}],"relatedPublications":[{"title":"Multifaceted profiling of virus-specific CD8 T cells reveals distinct immune signatures against cytomegalovirus infection states during pregnancy","doi":"https://doi.org/10.1016/j.isci.2025.112416","datasets":["JGAD000861"]}],"datasets":["JGAD000861"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000861"]}]}