{"id":"hum0464","version":2,"url":"https://humandbs.dbcls.jp/research/hum0464/v2","datePublished":"2025-06-24","versions":[{"version":1,"datePublished":"2024-07-25"},{"version":2,"datePublished":"2025-06-24"}],"title":{"ja":"ヒト造血器腫瘍細胞の体外増幅と病態再現モデルマウスの構築","en":"In Vitro Amplification of Human Hematopoietic Tumor Cells and Construction of Mouse Models for Reproduction of Pathological Conditions"},"summary":{"aims":{"ja":"白血病、骨髄異形成症候群、骨髄増殖性腫瘍、多発性骨髄腫などの造血器腫瘍に対する根治的治療法は十分に確立されているとは言い難い。これらの研究を阻む大きな要因の一つに、適切に病態を模倣するモデルマウスの構築の困難さがある。本研究では、難治性造血器腫瘍患者から末梢血または骨髄液を採取し、体外での増幅およびジェネティック・エピジェネティックスな観点を含めた分子生物学・細胞生物学的解析を試みて得た知見をもとに、患者検体移植造血器腫瘍モデルマウスの作製、さらに、新規治療法の開発を目指す。","en":"Radical treatments for hematopoietic tumors such as leukemia, myelodysplastic syndromes, myeloproliferative tumors, and multiple myeloma have not been fully established. One of the major factors hindering the efforts to find such treatments is the difficulty in constructing model mice that appropriately mimic the disease state. In this study, we collected peripheral blood or bone marrow cells from patients with hematological tumors and attempted in vitro cultures and molecular cell biological analyses, including genetic and epigenetic studies. Based on the knowledge gained from these studies, we aim to create patient-derived xenograft mouse models of hematological tumors and further develop new therapeutic approaches."},"methods":{"ja":"造血器腫瘍の確定診断および病勢評価のための骨髄穿刺液の一部を用いる。Ficoll処理により単核球を抽出し、MACSビーズもしくはFACS sorterを用いて目的の表面抗原を有する腫瘍細胞集団を分取する。これらの細胞を用いて、RNAシークエンスによる遺伝子発現プロファイル解析を行う。","en":"A part of bone marrow aspirates obtained to establish a definite diagnosis or evaluate the disease status was provided for this study. Mononuclear cells were separated by gradient separation using Ficoll, and then subjected to magnetic beads separation or FACS sorting to purify tumor cells. Next, they were subjected to RNA sequencing to analyze gene expression profiles."},"targets":{"ja":"【RNA-seq】 多発性骨髄腫17症例の骨髄86＋14検体、正常骨髄3検体\n【scRNA-seq、scBCR-seq】 多発性骨髄腫9症例の骨髄22検体","en":"[RNA-seq] A total of 86 + 14 bone marrow samples from 17 multiple myelomas, 3 normal bone marrow samples\n[single-cell RNA-seq, scBCR-seq] A total of 22 bone marrow samples from 9 multiple myelomas"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"多発性骨髄腫：21症例\n（日本人）\n正常骨髄：3検体\n（細胞株）","en":"multiple myeloma: 21 cases\n(Japanese)\nnormal bone marrow: 3 samples\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（RNA-seq、scRNA-seq、scBCR-seq）","en":"NGS\n(RNA-seq, scRNA-seq, scBCR-seq)"}},"releaseNote":{"ja":"多発性骨髄腫8症例の骨髄（腫瘍細胞）および購入した正常骨髄3検体から抽出したRNAを用いたRNA-seq解析データをfastqファイル形式で提供する。","en":"RNAs extracted from the born marrow of multiple myeloma patients and normal born marrow samples were used for RNA sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"岩間 厚志","en":"Atsushi Iwama"},"organization":{"name":{"ja":"東京大学 医科学研究所 幹細胞治療研究センター 幹細胞分子医学分野","en":"Division of Stem Cell and Molecular Medicine Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo"}}}],"researchProjects":[],"grants":[{"title":{"ja":"加齢造血幹細胞腫瘍における造血幹細胞エイジングの病因論的意義の解明","en":"Pathogenec implication of hematopoietic stem cell aging in age-associated hematological malignancies"},"agency":{"ja":"科学研究費助成事業 基盤研究（S）","en":"KAKENHI Grant-in-Aid for Scientific Research (S)"},"grantIds":["JP24H00066"]}],"relatedPublications":[],"datasets":["JGAD000856"],"controlledAccessUsers":[]}