{"id":"hum0461","secondaryIds":[],"version":1,"url":"https://humandbs.dbcls.jp/research/hum0461/v1","datePublished":"2024-05-31","versions":[{"version":1,"datePublished":"2024-05-31"},{"version":2,"datePublished":"2024-11-22"}],"title":{"ja":"食道扁平上皮癌、大腸癌に対する放射線治療後のがん免疫応答の解析","en":"Analysis of the cancer immune response following radiation therapy for esophageal squamous cell carcinoma and colorectal cancer"},"summary":{"aims":{"ja":"放射線治療（RT）はがん治療において重要な役割を果たし、患者の約50〜60％に施行される。従来、RTの主な作用機序は致死的なDNA損傷の誘導と考えられてきたが、最近の研究では免疫応答と免疫細胞の死が抗腫瘍効果に寄与することが明らかになっている。RTの単独使用では効果が制限されるため、免疫応答を活性化させるための新たな戦略が求められている。これには、免疫チェックポイント阻害剤（ICIs）をRTと組み合わせる臨床試験が行われているが、その最適な組み合わせに関する情報はほとんど明らかになっていない。本研究では、RT後のがん免疫応答の解析を通じて、免疫細胞の動態や遺伝子発現プロファイルを明らかにし、免疫療法や放射線治療の最適化に貢献することを目的とした。","en":"Radiotherapy (RT) is pivotal in cancer care, with 50 to 60% of patients receiving it. Traditionally, RT's primary mechanism was deemed to induce lethal DNA damage. However, recent findings unveil contributions from immune responses and immunological cell death to its anti-tumor efficacy. Since RT alone has limitations in maximizing these effects, novel strategies to activate immune responses are sought. Despite trials combining RT with immune checkpoint inhibitors (ICIs), optimal combinations remain elusive. This study aims to elucidate post-RT dynamics of cancer immune responses and gene expression profiles, aiming to optimize both immunotherapy and radiotherapy."},"methods":{"ja":"【scRNA-seq】Illuminaによるsingle cell RNAシークエンス\n【Spatial transcriptome seq】Illuminaによる空間トランスクリプトームVisiumシークエンスデータ","en":"【scRNA-seq】single cell sequencing by illumima\n【Spatial transcriptome seq】Spatial transcriptome sequencing Visium"},"targets":{"ja":"【scRNA-seq】食道扁平上皮がん：5例\n【Spatial transcriptome seq】食道扁平上皮がん：2例","en":"【scRNA-seq】esophageal squamous cell carcinoma: 5 cases\n【Spatial transcriptome seq】esophageal squamous cell carcinoma: 2 cases"},"url":{"ja":[{"url":"https://www.ncc.go.jp/jp/information/researchtopics/2023/1214/index.html","text":"https://www.ncc.go.jp/jp/information/researchtopics/2023/1214/index.html"}],"en":null}},"listingSummary":{},"releaseNote":{"ja":"食道扁平上皮がん5症例の腫瘍組織から抽出したRNAを用いたscRNA-seq解析ならびに食道扁平上皮がん2症例の腫瘍部位を含む組織切片を用いたVisium解析結果をfastq、png、json、mtx、csv、xlsx、tsvファイル形式で提供する。","en":"- RNAs extracted from tumor tissues of 5 esophageal squamous cell carcinoma patients were used for the single-cell RNA sequencing analysis. Fastq files are provided.\n- Tumor tissues from 2 esophageal squamous cell carcinoma patients were used for Visium Spatial Gene Expression Assays. Fastq, png, json, mtx, csv, xlsx, tsv files are provided."},"dataProviders":[{"name":{"ja":"影山 俊一郎","en":"Shun-Ichiro Kageyama"},"organization":{"name":{"ja":"国立がん研究センター東病院 放射線治療科","en":"Department of Radiation Oncology, National Cancer Center Hospital East"}}}],"researchProjects":[],"grants":[{"title":{"ja":"放射線誘導免疫応答におけるnon-coding RNA pathwayの解析","en":"Analysis of the Non-coding RNA Pathway in Radiation-induced Immune Responses"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["21K07582"]},{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Sciences"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["16H06279"]},{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Sciences"},"agency":{"ja":"科学研究費助成事業 学術変革領域研究","en":"KAKENHI Grant-in-Aid for Transformative Research Areas"},"grantIds":["22H04925"]}],"relatedPublications":[{"title":"Comprehensive single-cell analysis demonstrates radiotherapy-induced infiltration of macrophages expressing immunosuppressive genes into tumor in esophageal squamous cell carcinoma","doi":"https://doi.org/10.1126/sciadv.adh9069","datasets":["JGAD000845"]}],"datasets":["JGAD000845"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000845"]}]}