{"id":"hum0460","version":1,"url":"https://humandbs.dbcls.jp/research/hum0460/v1","datePublished":"2025-01-29","versions":[{"version":1,"datePublished":"2025-01-29"}],"title":{"ja":"尿路上皮癌の免疫チェックポイント阻害薬治療におけるcfDNAを用いたリキッドバイオプシーによるCompanion/Complimentary Diagnosisプラットフォームの開発に向けたパイロット研究","en":"Pilot study towards the development of a Companion/Complimentary Diagnosis platform using liquid biopsy with cfDNA for immune checkpoint inhibitor therapy in advanced urothelial cancer"},"summary":{"aims":{"ja":"膀胱癌・上部尿路上皮癌の原発巣組織を用いた遺伝子変異プロファイル、遺伝子変異量（TMB：Tumor Mutation Burden）、遺伝子発現プロファイル（分子サブタイプ診断）、免疫組織学的所見（PD-L1 発現）を予測可能なcell free DNA（cfDNA） ベースのプラットフォームの開発に向けた予備的解析を行い、その実現可能性を検証すること。","en":"Preliminary analysis was conducted to develop a cfDNA-based platform capable of predicting gene mutation profiles, TMB, gene expression profiles (molecular subtype diagnosis), and immunohistochemical findings (PD-L1 expression) using the primary lesion tissue of bladder cancer and upper urinary tract epithelial cancer, verifying the feasibility of such development."},"methods":{"ja":"Target captureシーケンス解析、RNAシーケンス解析","en":"Target capture sequencing, RNA sequencing"},"targets":{"ja":"進行性尿路上皮癌（膀胱癌・上部尿路上皮癌）：82症例","en":"advanced urothelial carcinoma (bladder cancer, upper urinary tract epithelial cancer) : 82 cases"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing; Expression profiling"},"targets":{"ja":"進行性尿路上皮癌（膀胱癌・上部尿路上皮癌）：82症例\n（日本人）","en":"advanced urothelial carcinoma: 82 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq、Target Capture）","en":"NGS\n(RNA-seq, Target Capture)"}},"releaseNote":{"ja":"進行性尿路上皮癌（膀胱癌・上部尿路上皮癌）82症例の血漿cell free DNA158検体ならびに腫瘍組織73検体から抽出したDNAを用いたTarget Capture Sequencing解析データ、および、腫瘍組織68検体から抽出したRNAを用いたRNA-seq解析データをbam、csv、Tabファイルにて提供する。","en":"cfDNA and DNA/RNA extracted from tumor tissues obtained from 82 advanced urothelial carcinoma patients were used for target capture sequencing and RNA sequencing analyses. Bam, csv and tab files are provided."},"dataProviders":[{"name":{"ja":"小林 恭","en":"Takashi Kobayashi"},"organization":{"name":{"ja":"京都大学大学院医学研究科 泌尿器科学","en":"Department of Urology, Kyoto University Graduate School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"尿路上皮癌の免疫チェックポイント阻害薬治療におけるcfDNAを用いたリキッドバイオプシーによるCompanion/Complimentary Diagnosisプラットフォームの開発に向けたパイロット研究","en":"Pilot study towards the development of a Companion/Complimentary Diagnosis platform using liquid biopsy with cfDNA for immune checkpoint inhibitor therapy in advanced urothelial cancer"},"agency":{"ja":"中外製薬株式会社","en":"Chugai Pharmaceutical Co., Ltd."},"grantIds":["R2636"]}],"relatedPublications":[{"title":"Clinical Utility of Serial Circulating Tumor DNA Analysis as a Minimally Invasive Biomarker in Advanced Urothelial Cancer","doi":"https://doi.org/10.1200/po-24-00472","datasets":["JGAD000847"]}],"datasets":["JGAD000847"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000847"]}]}