{"id":"hum0456","version":1,"url":"https://humandbs.dbcls.jp/research/hum0456/v1","datePublished":"2024-07-17","versions":[{"version":1,"datePublished":"2024-07-17"}],"title":{"ja":"傍鞍部頭蓋底腫瘍及び、悪性神経膠腫組織を用いた分子生物学的解析による腫瘍の形成と機能的分化における分子病態の検討","en":"Molecular Pathological exprolation of Tumor Formation and Functional Differentiation Using Parasellar Skull Base Tumors and Malignant Glioma Tissues"},"summary":{"aims":{"ja":"傍鞍部頭蓋底腫瘍や悪性神経膠腫の遺伝子情報と臨床情報の総合的な解析により、病態機序や病勢を規定する遺伝因子を解明することで、予防やオーダーメイド医療につながる臨床上有用な結果を得られると期待される。","en":"Comprehensive analysis of genetic and clinical information on parasellar base skull tumors and multiple malignant gliomas is expected to elucidate the pathogenesis of the diseases and provide clinically useful results leading to prevention and the development of personalized medicine."},"methods":{"ja":"摘出した腫瘍検体から得られた細胞懸濁液をChromium Controllerにより処理し、10×Chromium Next GEM single-cell 3ʹ試薬キットとライブラリー構築キットを使用して調製・定量・品質評価後、NovaSeq 6000にてsingle cell RNA-seqを実施","en":"The cell suspension from the resected tumor specimens was processed according to 10×Genomics specifications, using the 10×Chromium Next GEM single-cell 3ʹ reagent kit and library construction kit. After quantification and quality assessment of the library, single-cell RNA sequencing was performed on the NovaSeq 6000."},"targets":{"ja":"頭蓋咽頭腫10症例の手術摘出検体","en":"Surgical resection specimens from 10 craniopharyngiomas"},"url":{"ja":[{"url":"https://www.m.chiba-u.ac.jp/class/moldiag","text":"https://www.m.chiba-u.ac.jp/class/moldiag"},{"url":"https://www.ho.chiba-u.ac.jp/dept/neurosurgery/","text":"https://www.ho.chiba-u.ac.jp/dept/neurosurgery/"}],"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"頭蓋咽頭腫：10症例\n（日本人）","en":"craniopharyngioma: 10 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"頭蓋咽頭腫10症例の手術摘出検体の腫瘍組織から抽出したRNAを用いたscRNA-seq解析データをfastqファイルにて提供する。","en":"RNAs extracted from the cell suspension from the resected tumor specimens of 10 craniopharyngiomas were used for single-cell RNA-sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"松田 達磨","en":"Tatsuma Matsuda"},"organization":{"name":{"ja":"千葉大学大学院医学研究院 分子病態解析学","en":"Department of Molecular Diagnosis, Graduate School of Medicine Chiba University"}}}],"researchProjects":[{"name":{"ja":"傍鞍部頭蓋底腫瘍及び、悪性神経膠腫組織を用いた分子生物学的解析による腫瘍の形成と機能的分化における分子病態の検討","en":"Molecular Pathological exprolation of Tumor Formation and Functional Differentiation Using Parasellar Skull Base Tumors and Malignant Glioma Tissues"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"脂肪や血管組織老化に伴う機能変容における老化関連長鎖非コードRNAの基盤的研究","en":"Fundamental research on age-related long non-coding RNAs in functional alterations associated with adipose and vascular tissue ageing"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["21H02974"]},{"title":{"ja":"ミトコンドリア複合体と制御メカニズム解析から捉える肥満・糖尿病の分子病態研究","en":"Molecular pathological research on obesity and diabetes based on analysis of mitochondrial complexes and regulatory mechanisms"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["19H03708"]},{"title":{"ja":"p53転写因子複合体によるクロマチン機能調節とiPSリプログラム制御機構の解明","en":"Regulation of chromatin function and iPS reprogramming by the p53 transcription factor complex"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["22300325"]}],"relatedPublications":[{"title":"Deciphering craniopharyngioma subtypes: Single-cell analysis of tumor microenvironment and immune networks","doi":"https://doi.org/10.1016/j.isci.2024.111068","datasets":["JGAD000855"]}],"datasets":["JGAD000855"],"controlledAccessUsers":[]}