{"id":"hum0454","version":1,"url":"https://humandbs.dbcls.jp/research/hum0454/v1","datePublished":"2024-12-16","versions":[{"version":1,"datePublished":"2024-12-16"}],"title":{"ja":"免疫異常に着目した炎症性腸疾患の病因解析および治療反応性解析","en":"Etiological and therapeutic response analysis of inflammatory bowel disease focusing on immune abnormalities"},"summary":{"aims":{"ja":"Crohn病は原因不明の炎症性腸疾患であるが、リンパ管の異常やリンパ流の障害が病因に関連すると報告されている。一方、肉芽腫はCrohn病の組織学的特徴の一つであるが、肉芽腫がしばしばリンパ管内にみられ、リンパ管内の肉芽腫がCrohn病におけるリンパ流の障害の原因であるとの指摘もある。以上のようにCrohn病の病態を考える上で、リンパ管内の肉芽腫は重要な免疫学的構造物であるが、なぜリンパ管の中に存在し、どのような性質を持つかなどはほとんどわかっていない。本研究においては、リンパ管内およびリンパ管外のリンパ腫の性質を明らかにするとともに、リンパ管内およびリンパ管外肉芽腫とCrohn病における臨床病理学的な所見との関連性の有無の検証を行う。また生物学的製剤投与によるCrohn病における免疫応答の変化の解析も行う。","en":"Crohn's disease is one of inflammatory bowel diseases with unknown etiology, however dysfunction of lymphatic vessels has been reported to be associated with its etiology. Whereas, epithelioid cell granuloma is often found in lymphatic vessel and it is suggested that granulomas in lymphatic vessels are responsible for the impaired lymphatic flow in Crohn's disease. As described above, granulomas in lymphatic vessels are important immunological structures in the pathogenesis of Crohn's disease, but little is known about its characteristics. In this study, we will clarify the characteristics of intra- and extra- lymphatic granulomas and examine the correlation between intra- and extra- lymphatic granulomas and clinicopathological findings of Crohn's disease. We analyzed changes in the immune response in Crohn's disease after administering biologics as well."},"methods":{"ja":"Xenium解析（multi tissue panelの377分子＋カスタム50分子）","en":"Formalin-fixed paraffin-embedded specimens (FFPE) was thin sliced with 5 µm thickness and mounted on slides for Xenium analysis, 4 cases per slide. 50 custom molecules were added to the 377 molecules in the multi tissue panel for Xenium analysis."},"targets":{"ja":"Crohn病10症例およびコントロール6症例（潰瘍性大腸炎4症例、サイトメガロウィルス腸炎1症例、がん1症例）のホルマリン固定パラフィン包埋検体（FFPE）","en":"FFPE of Crohn's disease surgery cases, 10 cases. As controls, 6 cases of FFPE from ulcerative colitis, cytomegalovirus enteritis, and cancer were used."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"Crohn病：10症例\n潰瘍性大腸炎：4症例\nサイトメガロウィルス腸炎：1症例\n癌：1症例\n（日本人）","en":"Crohn's disease: 10 cases\nulcerative colitis: 4 cases\ncytomegalovirus enteritis: 1 case\ncancer: 1 case\n(Japanese)"},"typeOfData":{"ja":"Xenium In Situ Gene Expression","en":"Xenium In Situ Gene Expression"}},"releaseNote":{"ja":"Crohn病10症例、潰瘍性大腸炎4症例、サイトメガロウィルス腸炎1症例、がん1症例の手術摘出検体（FFPE）を用いたXenium空間トランスクリプトーム解析の結果をcsv、h5、html、json、mtx、tiff、parquet、tsv、xenium、zarrファイルにて提供する。","en":"Surgical sections (FFPE) from 10 patients with Crohn's disease, 6 patients with ulcerative colitis, cytomegalovirus enteritis, and cancer were used for Xenium In Situ Gene Expression Assay. Csv, h5, html, json, mtx, tiff, parquet, tsv, xenium, and zarr files are provided."},"dataProviders":[{"name":{"ja":"児玉 真","en":"Makoto Kodama"},"organization":{"name":{"ja":"東京山手メディカルセンター 病理診断科","en":"Department of Pathology, Tokyo Yamate Medical Center"}}}],"researchProjects":[],"grants":[{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Sciences"},"agency":{"ja":"科学研究費助成事業 学術変革領域研究","en":"KAKENHI Grant-in-Aid for Transformative Research Areas"},"grantIds":["22H04925"]}],"relatedPublications":[{"title":"Tertiary lymphoid structure as a therapeutic target of Crohn's disease: modulation by TNFα blockade","doi":"https://doi.org/10.1101/2024.11.23.625032","datasets":["JGAD000830"]}],"datasets":["JGAD000830"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"細川 浩之","en":"Hiroyuki Hosokawa"},"affiliation":{"ja":"探索研究部, EAファーマ株式会社","en":"Exploratory Research Department, EA Pharma Co., Ltd."},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"Crohn病におけるリンパ管内の肉芽腫の性状解析","en":"Characterization of Granulomas in Lymphatic Vessels in Crohn's Disease"},"periodStart":"2025-11-17","periodEnd":"2026-04-02","datasets":["JGAD000830"]}]}