{"id":"hum0435","version":1,"url":"https://humandbs.dbcls.jp/research/hum0435/v1","datePublished":"2024-03-18","versions":[{"version":1,"datePublished":"2024-03-18"}],"title":{"ja":"iPS細胞由来心筋細胞における網羅的核酸解析を用いた再生医療用細胞作製方法の検討","en":"Investigation of a method for generating cells for regenerative medicine using comprehensive nucleic acid analysis of iPS cell-derived cardiomyocytes"},"summary":{"aims":{"ja":"重症心不全に対する新しい治療法として細胞移植を中心とした再生医療が期待されている。本治療法では、ヒトiPS細胞を用いて質の高い心筋細胞を大量に作製し、心不全で失われた心筋細胞を補填することにより心機能の回復が期待されている。当研究室では、世界で初めてヒトiPS細胞から作製した心筋細胞を心筋梗塞モデルブタに移植し、病状が改善することを示した。しかしながら、iPS細胞の分化効率の不安定性およびiPS細胞移植後の造腫瘍性は未だ解決すべき課題である。そのため、安定的な心筋細胞分化誘導を目指し、iPS細胞の心筋細胞への分化誘導に関連する遺伝子群、およびDNAのメチル化の同定を試みる。さらに、ヒトiPS細胞から分化誘導した心筋細胞において腫瘍形成に関わる遺伝子を網羅的に解析し、移植前に移植用細胞の安全性を担保する為の腫瘍形成予測マーカーを開発することを目的とする。","en":"Regenerative medicine for heart failure is expected to restore cardiac function by using human iPS cells (hiPSCs). In our laboratory, it was shown that transplantation of cardiomyocytes (CMs) derived from hiPSCs into a pig model of myocardial infarction improves the disease condition. However, the instability of differentiation efficiency and tumorigenicity after transplantation are still issues to be resolved. We attempt to identify and analyze the genes and DNA methylation associated with the induction of differentiation of hiPSCs into CMs to achieve stable induction of CMs differentiation. Furthermore, we aim to analyze genes involved in tumorigenesis in CMs, and to develop a predictive marker for tumorigenesis to ensure the safety of cells before transplantation."},"methods":{"ja":"ヒトiPS細胞から心筋細胞を分化誘導し、single cell RNA-seq解析を実施した","en":"Cardiomyocytes were induced from human iPS cells and gene expression was analyzed by single cell RNA-seq."},"targets":{"ja":"HLAホモ健常ドナー1名の末梢血細胞から樹立したヒトiPS細胞を分化誘導した心筋細胞","en":"Cardiomyocytes differentiated from human iPS cells established from peripheral blood cells of a healthy donor"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"HLAホモ健常ドナー1名の細胞から樹立されたヒトiPS細胞から分化誘導した心筋細胞：1検体\n（細胞株）","en":"Cardiomyocytes differentiated from human iPS cells established from healthy donor cells: 1 samples\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"HLAホモ健常ドナー1名の末梢血細胞から樹立したヒトiPS細胞を分化誘導して作製した心筋細胞を用いたsingle cell RNA-seq解析データをfastqファイルおよびtextファイルにて提供する。","en":"RNAs extracted from a cardiomyocyte differentiated from human iPS cells established from a healthy donor's peripheral blood cells were used for single-cell RNA-sequencing analysis. Fastq and text files are provided."},"dataProviders":[{"name":{"ja":"宮川 繁","en":"Shigeru Miyagawa"},"organization":{"name":{"ja":"大阪大学大学院医学系研究科 心臓血管外科学","en":"Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"iPS細胞を用いた心筋再生治療創成拠点","en":"Center for the development of myocardial regenerative treatments using iPS cells"},"agency":{"ja":"日本医療研究開発機構（AMED） 再生医療実現拠点ネットワークプログラム","en":"Research Center Network for Realization of Regenerative Medicine, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP20bm0204003"]}],"relatedPublications":[{"title":"Pre-clinical evaluation of the efficacy and safety of human induced pluripotent stem cell-derived cardiomyocyte patch","doi":"https://doi.org/10.1186/s13287-024-03690-8","datasets":["JGAD000795"]}],"datasets":["JGAD000795"],"controlledAccessUsers":[]}