{"id":"hum0430","version":1,"url":"https://humandbs.dbcls.jp/research/hum0430/v1","datePublished":"2025-12-02","versions":[{"version":1,"datePublished":"2025-12-02"}],"title":{"ja":"大腸疾患における腸内環境理解のための内視鏡的生検バンキングの構築とそれらを用いた探索的研究","en":"Construction of endoscopic biopsy banking for understanding the intestinal environment in colorectal diseases and exploratory studies using these banking systems."},"summary":{"aims":{"ja":"大腸がんは本邦で最も罹患者の多いがん種である。一部の大腸がんは遺伝的背景を有するが、大腸がんの多くは散発性である。大腸がんのリスクファクターとしては喫煙、肥満、運動不足、赤肉と加工肉の摂取などが知られている。さらに、食生活や生活習慣の変化による腸内環境の変化が本邦の大腸がんの発症率上昇に関与している可能性が示唆されているものの、その分子機構はまだ十分に解明されていない。本研究は、大腸腫瘍をひきおこす腸内環境の変化の解明を主な目的としている。","en":"Colorectal cancer is the most common type of cancer in Japan. Although some colorectal cancers have a genetic background, most colorectal cancers are sporadic. Known risk factors for colorectal cancer include smoking, obesity, lack of exercise, and intake red or processed meat. Furthermore, although it has been suggested that changes in the intestinal environment due to changes in diet and lifestyle may be involved in the increased incidence of colorectal cancer in Japan, the molecular mechanism has not yet been fully elucidated. The main objective of this study is to elucidate the changes in the intestinal environment that cause colorectal tumors."},"methods":{"ja":"大腸内視鏡を用いて、腫瘍組織、腫瘍部から1-2cm離れた正常粘膜組織、回腸末端・盲腸・上行結腸・下行結腸・S状結腸・直腸の各部位から採取した。生検検体からDNA/RNAを抽出し、Whole Exome Sequencing解析ならびにRNA-seq解析を行った。","en":"Research biopsies were obtained from the tumor, normal mucosa 1-2 cm away from the tumor, terminal ileum, cecum, ascending colon, descending colon, and sigmoid colon to rectum using a lower gastrointestinal endoscopy for participants. DNA/RNA was extracted from the samples and used for RNA-seq and whole exome sequencing analyses."},"targets":{"ja":"RNA-seq：進行大腸がん14症例、早期大腸がん41症例、大腸腺腫6症例、健常者17名\nExome：進行大腸がん24症例（14症例はRNA-seqも実施）、早期大腸がん23症例（20症例はRNA-seqも実施）","en":"RNA-seq: 14 patients with advanced colorectal cancer, 41 patients with early stage colorectal cancer, 6 patients with colorectal adenoma, and 17 healthy individuals\nExome: 24 patients with advanced colorectal cancer (including 14 same cases in RNA-seq), 23 patients with early stage colorectal cancer (including 20 same cases in RNA-seq)"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"大腸癌：68症例\n大腸腺腫：6症例\n健常者：17名\n（日本人）","en":"colorectal cancer: 68 cases\ncolorectal adenoma: 6 cases\n17 healthy subjects\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq）","en":"NGS\n(Exome, RNA-seq)"}},"releaseNote":{"ja":"大腸がん68症例、大腸腺腫6症例、健常者17名の腫瘍組織、大腸腺腫、正常腸管粘膜組織から抽出したDNA/RNAを用いたwhole exome sequencing解析ならびにRNA sequencing解析データをfastqファイルにて提供する。","en":"DNA/RNA extracted from the colorectal cancer, colorectal adenoma, and normal intestinal mucosa samples obtained from patients with colorectal cancer, colorectal adenoma patients, and healthy individuals were used for the whole exome sequencing and RNA sequencing analyses. Fastq files are provided."},"dataProviders":[{"name":{"ja":"谷内田 真一","en":"Shinichi Yachida"},"organization":{"name":{"ja":"国立がん研究センター研究所 がんゲノミクス研究分野","en":"Division of Cancer Genomics, National Cancer Center Research Institute"}}}],"researchProjects":[{"name":{"ja":"大腸疾患における腸内環境理解のための内視鏡的生検バンキングの構築とそれらを用いた探索的研究","en":"Establishment of an Endoscopic Biopsy Repository for Comprehensive Characterization of the Gut Microenvironment in Colorectal Diseases and Its Application to Exploratory Translational Research"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"多施設共同による大腸がんならびに発がんハイリスクグループにおける腸内微生物叢のメタゲノムデータベースの基盤構築","en":"Establishment of a comprehensive metagenomic database of the gut microbiota in colorectal cancer patients and individuals at high risk for cancer development: A multi-institutional collaborative study"},"agency":{"ja":"国立がん研究センターがん研究開発費","en":"National Cancer Center Research and Development Fund"},"grantIds":["2020-A-4"]},{"title":{"ja":"多施設共同による便ならびに全消化管内視鏡下生検バンキングの基盤構築に関する研究","en":"Establishment of the infrastructure for a multicenter biobank of fecal and comprehensive gastrointestinal endoscopic biopsy specimens"},"agency":{"ja":"国立がん研究センターがん研究開発費","en":"National Cancer Center Research and Development Fund"},"grantIds":["2023-A-6"]},{"title":{"ja":"環状ペプチド誘導体を用いた標的選択的な中分子医薬品の創製研究","en":"Development of target-selective mid-size drugs using cyclic peptide derivatives"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["20H03362"]},{"title":{"ja":"大腸Serrated pathwayにおける腸内環境変動の解明","en":"Studies on the elucidation of intestinal environmental changes in colorectal tumorigenesis via the serrated pathway"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["23H02892"]},{"title":{"ja":"大腸癌ハイリスク患者の癌発症に関わる腸内環境変動の解明とそれに基づく大腸癌個別化予防法の開発に関する研究","en":"Research aimed at elucidating alterations in the intestinal microenvironment associated with colorectal cancer development in high-risk individuals, and the development of personalized preventive strategies based on these findings"},"agency":{"ja":"日本医療研究開発機構（AMED）革新的がん医療実用化研究事業","en":"Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP22ck0106546"]},{"title":{"ja":"大腸癌超ハイリスク群における便ならびに生検組織を用いた腸内環境理解に基づく大腸癌個別化予防法の開発","en":"Development of personalized colorectal cancer prevention strategies through integrated profiling of the gut microbiome using stool and endoscopic biopsy specimens in ultra-high-risk populations"},"agency":{"ja":"日本医療研究開発機構（AMED）革新的がん医療実用化研究事業","en":"Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP23ck0106799"]},{"title":{"ja":"腸内細菌解析による抗悪性腫瘍剤の有害作用発現予測に関する研究開発","en":"Research and development aimed at predicting the occurrence of adverse effects associated with anticancer drugs through comprehensive analysis of the gut microbiota"},"agency":{"ja":"日本医療研究開発機構（AMED）次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP21cm0106477"]},{"title":{"ja":"腸内環境解析による若年発症大腸癌の易罹患性・早期診断バイオマーカーの開発","en":"Development of biomarkers for early detection and susceptibility assessment of young-onset colorectal cancer through comprehensive analysis of the intestinal microenvironment"},"agency":{"ja":"日本医療研究開発機構（AMED）次世代がん医療加速化研究事業（P-PROMOTE）","en":"Project for Promotion of Cancer Research and Therapeutic Evolution (P-PROMOTE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP23ama221404"]},{"title":{"ja":"食事、腸内細菌、炎症の多面的解析に基づく若年発症大腸癌の本態解明と易罹患性・早期診断検査法の開発","en":"Elucidation of the pathogenetic mechanisms underlying early-onset colorectal cancer through integrated analyses of diet, gut microbiota, and mucosal inflammation, and the development of novel susceptibility and early diagnostic strategies"},"agency":{"ja":"日本医療研究開発機構（AMED）次世代がん医療加速化研究事業（P-PROMOTE）","en":"Project for Promotion of Cancer Research and Therapeutic Evolution (P-PROMOTE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP24ama221430"]},{"title":{"ja":"日米医学協力計画を基軸とした感染関連がんに関する疾患研究","en":"Investigation of infection-associated cancers conducted within the framework of the Japan-U.S. Medical Cooperation Program"},"agency":{"ja":"日本医療研究開発機構（AMED）地球規模保健課題解決推進のための研究事業","en":"Research Program on the challenges of Global Health issues, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP23jk0210009"]},{"title":{"ja":"肉腫・脳腫瘍などの希少がんを対象としたゲノム解析による予防法・診断法・治療法の開発","en":"Development of novel strategies for prevention, diagnosis, and treatment through comprehensive genomic analysis of rare cancers, including sarcomas and brain tumors"},"agency":{"ja":"日本医療研究開発機構（AMED）革新的がん医療実用化研究事業","en":"Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP24ck0106877"]},{"title":{"ja":null,"en":null},"agency":{"ja":"大阪大学先導的学際研究機構 生命医科学融合フロンティア研究部門","en":"Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University"},"grantIds":null},{"title":{"ja":"新規の大腸癌発癌経路であるSerrated pathwayにおける腸内細菌の関与に関する研究","en":"Investigation of the role of the gut microbiota in the serrated pathway, a novel colorectal carcinogenesis mechanism"},"agency":{"ja":"東京大学医科学研究所 共同研究","en":"Joint Research Project of the Institute Medical Science, the University of Tokyo"},"grantIds":null},{"title":{"ja":"\"Microbiome-based Precision Medicine\"を見据えた腸内細菌叢の変動に基づく大腸癌発症機構の解明と予防法の創出","en":"Elucidation of colorectal carcinogenesis mechanisms and development of preventive strategies based on gut microbiome dynamics toward the implementation of microbiome-driven precision medicine"},"agency":{"ja":"公益財団法人 武田科学振興財団","en":"Takeda Science Foundation"},"grantIds":null},{"title":{"ja":"腸内微生物叢の変動に基づく大腸癌発症機構の解明","en":"Elucidation of the mechanisms underlying colorectal carcinogenesis based on gut microbiome dynamics"},"agency":{"ja":"公益財団法人 安田記念医学財団","en":"Yasuda Medical Foundation"},"grantIds":null},{"title":{"ja":"大腸腫瘍発生に関与する腸内細菌の特定とそのメカニズムの解明","en":"Identification of gut microbial agents involved in colorectal tumorigenesis and elucidation of their underlying mechanistic pathways"},"agency":{"ja":"公益財団法人 三菱財団","en":"The Mitsubishi Foundation"},"grantIds":null},{"title":{"ja":"腸内微生物が大腸癌発症に及ぼす影響に関する研究","en":"Investigation of the impact of gut microorganisms on the pathogenesis of colorectal cancer"},"agency":{"ja":"公益財団法人 高松宮妃癌研究基金","en":"Princess Takamatsu Cancer Research Fund"},"grantIds":null},{"title":{"ja":"大腸癌発症予防に向けた腸内環境制御に関する研究","en":"Investigation of intestinal microenvironment modulation for the prevention of colorectal cancer"},"agency":{"ja":"公益財団法人 ヤクルト・バイオサイエンス研究財団","en":"Yakult Bio-Science Foundation"},"grantIds":null}],"relatedPublications":[{"title":"Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies","doi":"https://doi.org/10.1186/s12943-024-02159-9","datasets":["JGAD000785"]}],"datasets":["JGAD000785"],"controlledAccessUsers":[]}