{"id":"hum0427","secondaryIds":[],"version":1,"url":"https://humandbs.dbcls.jp/research/hum0427/v1","datePublished":"2023-11-02","versions":[{"version":1,"datePublished":"2023-11-02"},{"version":2,"datePublished":"2026-06-16"}],"title":{"ja":"頭頸部悪性腫瘍細胞株の樹立 / 頭頸部悪性腫瘍に対する網羅的遺伝子解析およびデータベースの構築","en":"Establishment of cell lines derived from head and neck malignancies / Comprehensive genetic analysis and database construction for head and neck malignancies"},"summary":{"aims":{"ja":"悪性腫瘍の研究において、多くの研究者が細胞バンクの細胞株を利用しているが、細胞バンクから入手可能な頭頚部悪性腫瘍の細胞株は部位、組織型ともに限られており、特に希少がんの細胞株入手は困難である。本研究では、頭頸部悪性腫瘍の細胞株を樹立後免疫不全動物へ移植し、生着・増殖の可否を検討する。また、樹立した細胞株は細胞バンクへ寄託・譲渡し、多くの研究者が利用可能なものとすることで、頭頚部悪性腫瘍、特に希少がんについて病態の解明や治療法確立に向けての研究を推進することを目的とする。","en":"In the field of oncology, many researchers generally use cell lines obtained from cell banks. However, cell lines for head and neck malignancies available from cell banks are limited especially for rare-type cancers, resulting in difficulties in elucidation of biological characterization of such malignancies and establishment of therapeutic strategies. This study aimed to establish cell lines of head and neck malignancies and to examine their tumorigenic abilities in immune-deficient animals. The established cell lines will be deposited to cell banks."},"methods":{"ja":"末梢血単核細胞、原発腫瘍組織、原発巣腫瘍組織から樹立した細胞株、それぞれから抽出したDNAを用いた全エクソーム配列決定","en":"Genomic DNAs extracted from peripheral blood mononuclear cells (PBMC), the primary tumor tissues, and the cell line were used for whole exome sequencing analysis."},"targets":{"ja":"外耳道扁平上皮がん患者1名から採取した末梢血単核細胞、腫瘍原発巣組織、および腫瘍原発巣組織から樹立した細胞株（計3検体）。細胞株は細胞株（SCEACono2）として理化学研究所バイオリソース研究センターに寄託済（https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB5515&lang=En）。","en":"PBMC and the primary tumor tissues were collected from a patient with external auditory canal squamous cell carcinoma, and a cell line was established from the primary tumor tissues. The cell line was deposited as SCEACono2 in RIKEN BioResource Research Center (https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB5515&lang=En)."},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"外耳道扁平上皮がん1症例の末梢血単核細胞、原発腫瘍組織、原発腫瘍組織から樹立した細胞株、それぞれから抽出したDNAを用いたwhole exome sequencing解析データをfastqファイルにて提供する。","en":"DNAs extracted from PBMC, the primary tumor tissues, and the cell line of tumor tissues from a patient with external auditory canal squamous cell carcinoma were used for the whole exome sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"中川 尚志","en":"Takashi Nakagawa"},"organization":{"name":{"ja":"九州大学大学院医学研究院 耳鼻咽喉科学分野","en":"Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"側頭骨扁平上皮癌の網羅的組織解析に基づく新規統合的治療戦略の開発","en":"Development of novel synthesizing treatment strategy by exhaustive research on temporal bone squamous cell carcinoma"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["18H02951"]},{"title":{"ja":"側頭骨扁平上皮癌の悪性度を決定するエピジェネティックな腫瘍制御機構の解明","en":"Elucidation of epigenetic tumor control mechanisms that determine the malignancy of squamous cell carcinoma of the temporal bone"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["22H03236"]}],"relatedPublications":[{"title":"Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma","doi":"https://doi.org/10.1002/2211-5463.13225","datasets":["JGAD000775"]}],"datasets":["JGAD000775"],"controlledAccessUsers":[]}