{"id":"hum0427","version":2,"url":"https://humandbs.dbcls.jp/research/hum0427/v2","datePublished":"2026-06-16","versions":[{"version":1,"datePublished":"2023-11-02"},{"version":2,"datePublished":"2026-06-16"}],"title":{"ja":"頭頸部悪性腫瘍細胞株の樹立 / 頭頸部悪性腫瘍に対する網羅的遺伝子解析およびデータベースの構築","en":"Establishment of cell lines derived from head and neck malignancies / Comprehensive genetic analysis and database construction for head and neck malignancies"},"summary":{"aims":{"ja":"頭頸部領域悪性腫瘍の発生部位は、咽頭、喉頭、口腔、鼻・副鼻腔、唾液腺、甲状腺、側頭骨等と多岐にわたり、それぞれの発生部位により分子生物学的特徴が異なる。そのため、それぞれの腫瘍の分子生物学的特性に合わせたテーラーメイドの治療の確立が必要である。しかしながら、多くの研究者が細胞バンクの細胞株を利用しているが、細胞バンクから入手可能な頭頚部悪性腫瘍の細胞株は部位、組織型ともに限られており、特に希少がんの細胞株入手は困難である。本研究では、①頭頸部悪性腫瘍組織および患者由来血液の網羅的遺伝子解析を行い、そのデータベースを構築すること、②頭頸部悪性腫瘍の細胞株を樹立後免疫不全動物へ移植し、生着・増殖の可否を検討すること、③樹立した細胞株を細胞バンクへ寄託・譲渡し、多くの研究者が利用可能にすることで、頭頚部悪性腫瘍、特に希少がんについて病態の解明や治療法確立に向けての研究を推進すること、を目的とする。各々の遺伝子解析で得られた知見を多角的に検討することにより、頭頸部悪性腫瘍特異的に関与するがん関連遺伝子や発生母地特異的にみられる遺伝子変異の検索を行うことで、がん関連遺伝子発現もしくは遺伝子変異と予後との相関を明らかにすることにより、頭頸部悪性腫瘍の発がんや進展のメカニズムの解明や、再発・転移予測マーカーの同定、新規分子標的治療の確立を目指す。","en":"- Head and neck malignancies originate in a wide variety of locations, including the pharynx, larynx, oral cavity, nose and paranasal sinuses, salivary glands, thyroid gland, and temporal bone. Their molecular biological characteristics differ depending on the location of origin. Therefore, it is necessary to establish a tailored treatment that is tailored to the molecular biological characteristics of each tumor. In the field of oncology, many researchers generally use cell lines obtained from cell banks. However, cell lines for head and neck malignancies available from cell banks are limited especially for rare-type cancers, resulting in difficulties in elucidation of biological characterization of such malignancies and establishment of therapeutic strategies. This study aimed to 1) conduct comprehensive genetic analysis of head and neck malignant tumor tissues and patient-derived blood, 2) establish cell lines of head and neck malignancies and to examine their tumorigenic abilities in immune-deficient animals, 3) deposit to cell banks. Using multi-omics approaches, we seek to elucidate the molecular mechanisms of oncogenes and to develop biomarkers for recurrence and metastasis prediction, as well as strategies for novel targeted therapies."},"methods":{"ja":"- 外耳道扁平上皮がん症例の末梢血単核細胞、原発腫瘍組織、原発巣腫瘍組織から樹立した細胞株、それぞれから抽出したDNAを用いた全エクソーム解析\n- 側頭骨扁平上皮がん症例の原発巣腫瘍組織、正常皮膚組織それぞれから抽出したDNAを対象とするH3K27AcおよびYAP抗体を用いたChIP-seq解析","en":"- Genomic DNAs extracted from peripheral blood mononuclear cells (PBMC), the primary tumor tissues, and the cell line were used for whole exome sequencing analysis.\n- Chromatin immunoprecipitation (ChIP) sequencing using H3K27Ac and YAP antibodies"},"targets":{"ja":"- 外耳道扁平上皮がん患者1名から採取した末梢血単核細胞、腫瘍原発巣組織、および腫瘍原発巣組織から樹立した細胞株。細胞株は細胞株（SCEACono2）として理化学研究所バイオリソース研究センターに寄託済（https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB5515&lang=En）。\n- 側頭骨扁平上皮がん5症例の原発巣腫瘍組織ならびに正常皮膚組織","en":"- PBMC and the primary tumor tissues were collected from a patient with external auditory canal squamous cell carcinoma, and a cell line was established from the primary tumor tissues. The cell line was deposited as SCEACono2 in RIKEN BioResource Research Center (https://cellbank.brc.riken.jp/cell_bank/CellInfo/?cellNo=RCB5515&lang=En).\n- Five patients with temporal bone squamous cell carcinoma"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\nヒストン修飾","en":"Sequencing, Histone modification profiling"},"targets":{"ja":"外耳道扁平上皮癌：1症例\n側頭骨扁平上皮癌：5症例\n（日本人）","en":"external auditory canal squamous cell carcinoma: 1 case\ntemporal bone squamous cell carcinoma: 5 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、ChIP-seq）","en":"NGS\n(Exome, ChIP-seq)"}},"releaseNote":{"ja":"側頭骨扁平上皮がん5症例の原発巣腫瘍組織ならびに正常皮膚組織より抽出したDNAに対してH3K27Ac抗体もしくはYAP抗体を用いたChIP-seq解析データをfastqファイルにて提供する。","en":"DNAs extracted from tumor and non-tumor tissues of 5 patients with temporal bone squamous cell carcinoma were used for ChIP-seq analysis using anti-H3K27Ac and YAP antibodies. Fastq files are provided."},"dataProviders":[{"name":{"ja":"中川 尚志","en":"Takashi Nakagawa"},"organization":{"name":{"ja":"九州大学大学院医学研究院 耳鼻咽喉科学分野","en":"Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"側頭骨扁平上皮癌の網羅的組織解析に基づく新規統合的治療戦略の開発","en":"Development of novel synthesizing treatment strategy by exhaustive research on temporal bone squamous cell carcinoma"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["18H02951"]},{"title":{"ja":"側頭骨扁平上皮癌の悪性度を決定するエピジェネティックな腫瘍制御機構の解明","en":"Elucidation of epigenetic tumor control mechanisms that determine the malignancy of squamous cell carcinoma of the temporal bone"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["22H03236"]},{"title":{"ja":"側頭骨扁平上皮癌におけるNLRP3インフラマゾーム機構を介した転移制御機構の解明","en":"Elucidation of the Metastasis Regulatory Mechanism via NLRP3 Inflammasome Pathway in Temporal Bone Squamous Cell Carcinoma"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["22K09745"]},{"title":{"ja":"頭頸部扁平上皮癌の浸潤・転移に関わる転写リプログラミング機構の解明","en":"Investigation of transcriptional reprogramming mechanisms involved in invasion and metastasis of head and neck squamous cell caricnoma"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"KAKENHI Grant-in-Aid for Early-Career Scientists"},"grantIds":["20K18300"]},{"title":{"ja":"マルチオミクス解析による外耳道扁平上皮癌の進展に寄与する転写制御機構の解明","en":"Elucidation of Transcriptional Regulatory Mechanisms Contributing to the Progression of Squamous Cell Carcinoma of the External Auditory Canal via Multi-omics Analysis"},"agency":{"ja":"一般財団法人 曽田豊二記念財団 研究助成金","en":"Research Grant, Soda Toyoji Memorial Foundation"},"grantIds":null},{"title":{"ja":"頭頸部扁平上皮癌の浸潤及び転移に寄与するYAP1を介した転写リプログラミング機構の解明","en":"Elucidation of the YAP1-mediated transcriptional reprogramming mechanism contributing to invasion and metastasis in head and neck squamous cell carcinoma"},"agency":{"ja":"公益財団法人 武田科学振興財団 医学系研究助成","en":"Medical Research Grant, Takeda Science Foundation"},"grantIds":null},{"title":{"ja":"多層オミックス解析による頭頸部がんの進展に関わる転写リプログラミング機構の解明","en":"Exploring Transcriptional Reprogramming Mechanisms Underlying Head and Neck Cancer Progression via Multi-omics Analysis"},"agency":{"ja":"公益財団法人新日本先進医療研究財団 研究助成金","en":"Cancer Research Grant, Shinnihon Foundation of Advanced Medical Treatment Research"},"grantIds":null},{"title":{"ja":"頭頸部扁平上皮癌の浸潤及び転移に寄与する転写リプログラミング機構の解明","en":"Elucidation of Transcriptional Reprogramming Mechanisms involved in Invasion and Metastasis in Head and Neck Squamous Cell Carcinoma"},"agency":{"ja":"公益財団法人SGH財団 第33回SGHがん研究助成","en":"Cancer Research Grant, SGH Foundation"},"grantIds":null}],"relatedPublications":[{"title":"Establishment and characterization of a primary cell culture derived from external auditory canal squamous cell carcinoma","doi":"https://doi.org/10.1002/2211-5463.13225","datasets":["JGAD000775"]},{"title":"Aberrant Hippo-YAP/TEAD Signaling Drives Malignant Transcriptional Reprogramming in External Auditory Canal Squamous Cell Carcinoma","doi":"https://doi.org/10.1158/2767-9764.crc-25-0626","datasets":["JGAD000775"]}],"datasets":["JGAD000775"],"controlledAccessUsers":[]}