{"id":"hum0419","version":4,"url":"https://humandbs.dbcls.jp/research/hum0419/v4","datePublished":"2025-03-21","versions":[{"version":1,"datePublished":"2024-04-24"},{"version":2,"datePublished":"2024-05-08"},{"version":3,"datePublished":"2024-06-17"},{"version":4,"datePublished":"2025-03-21"}],"title":{"ja":"ヒト消化器癌、胸部悪性腫瘍オルガノイド培養系のゲノム医療への応用","en":"Application of organoid culture system of human gastrointestinal and thoracic cancer in genome medicine"},"summary":{"aims":{"ja":"消化器がんおよび胸部悪性腫瘍由来オルガノイドを作製し、ゲノム解析情報から有効な抗がん剤・分子標的薬剤を選定し、その抗腫瘍効果を解析することで、個別化医療における薬剤評価系の構築を行う。さらに、正常組織由来オルガノイドに遺伝子改変を加えることで、発癌に関わる分子生物学的機序を明らかにする。","en":"We generated organoids derived from gastrointestinal cancer and intra-thoracic malignancy, and selected effective anticancer agents and molecular targeted drugs based on genome analysis. In addition, we analyze their antitumor effects and construct a drug evaluation system for personalized medicine. Furthermore, by genetically modifying normal lung organoids, we elucidate the molecular mechanisms involved in the carcinogenesis of lung cancer."},"methods":{"ja":"全エクソームシーケンスおよびRNAシーケンス解析","en":"Whole exome sequencing and RNA sequencing analysis"},"targets":{"ja":"肺大細胞神経内分泌がん症例の腫瘍細胞より樹立したオルガノイド、粘液産生能を有する充実型肺腺がん患者より樹立したオルガノイド、EML4-ALK variant 3（v3）をドライバー遺伝子にもつ肺腺がん患者より樹立したオルガノイド、食道がん患者より樹立したオルガノイド","en":"Organoids established from patients with pulmonary large-cell neuroendocrine carcinoma, a patient with mucus-producing solid-dominant adenocarcinoma of lung and a patient with pulmonary adenocarcinoma harboring EML4-ALK variant 3, organoids established from patients with esophageal cancer"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"肺大細胞神経内分泌癌：3症例\n粘液産生能を有する充実型肺腺癌：1症例\nEML4-ALK variant 3（v3）をドライバー遺伝子にもつ肺腺癌：1症例\n食道癌：3症例\n（日本人）","en":"pulmonary large cell neuroendocrine carcinoma: 3 cases\nmucus-producing solid-dominant adenocarcinoma of lung: 1 case\npulmonary adenocarcinoma harboring EML4-ALK variant 3: 1 case\nesophageal cancer: 3 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq）","en":"NGS\n(Exome, RNA-seq)"}},"releaseNote":{"ja":"食道がん3症例の腫瘍組織より樹立したオルガノイドから抽出したDNAを用いたwhole exome sequencing解析データをbam、baiファイルにて提供する。","en":"DNAs extracted from organoids established from 3 patients with esophageal cancer were used for the whole exome sequencing analysis. Bam and bai files are provided."},"dataProviders":[{"name":{"ja":"深澤 拓也","en":"Takuya Fukazawa"},"organization":{"name":{"ja":"川崎医科大学 総合外科","en":"Department of General Surgery, Kawasaki Medical School"}}}],"researchProjects":[],"grants":[{"title":{"ja":"オルガノイド技術を用いた肺扁平上皮癌の発癌、進展機序の解明","en":"Elucidation of mechanism of carcinogenesis and progression in lung squamous cell carcinoma with organoid technology"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K07699"]},{"title":{"ja":"オルガノイドを用いた肺大細胞神経内分泌癌の分子病態および発癌メカニズムの解明","en":"Elucidation of the molecular pathogenesis and carcinogenic mechanisms of pulmonary neuroendocrine tumors using organoids"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["22H03164"]}],"relatedPublications":[{"title":"Patient-derived tumoroid models of pulmonary large-cell neuroendocrine carcinoma: A promising tool for personalized medicine and developing novel therapeutic strategies","doi":"https://doi.org/10.1016/j.canlet.2024.216816","datasets":["JGAD000771"]},{"title":"Establishment and characterization of novel high mucus-producing lung tumoroids derived from a patient with pulmonary solid adenocarcinoma","doi":"https://doi.org/10.1007/s13577-024-01060-3","datasets":["JGAD000783"]},{"title":"Transforming tumoroids derived from ALK-positive pulmonary adenocarcinoma to squamous cell carcinoma in vivo","doi":"https://doi.org/10.1007/s13577-024-01085-8","datasets":["JGAD000794"]},{"title":"Establishment and characterization of novel patient-derived esophageal tumoroids with long-term cultivability","doi":"https://doi.org/10.1007/s13577-025-01206-x","datasets":["JGAD000884"]}],"datasets":["JGAD000771","JGAD000783","JGAD000794","JGAD000884"],"controlledAccessUsers":[]}