{"id":"hum0416","version":3,"url":"https://humandbs.dbcls.jp/research/hum0416/v3","datePublished":"2024-10-16","versions":[{"version":1,"datePublished":"2023-08-10"},{"version":2,"datePublished":"2024-09-27"},{"version":3,"datePublished":"2024-10-16"},{"version":4,"datePublished":"2025-05-19"},{"version":5,"datePublished":"2025-06-06"}],"title":{"ja":"経時的な多層的オミックス解析を通した免疫難病及びがんにおける生体反応の解明と新規治療法の開発","en":"Elucidation of biological responses in intractable immune disorders and cancer and development of novel therapies through sequential multilevel omics analysis."},"summary":{"aims":{"ja":"免疫反応が関与する疾病（自己免疫及び難治性免疫疾患や、線維化を伴う疾患、がんなど）について、体内の生体反応や病態をより詳細に把握するため、血液及び組織標本を用いた経時的な変化と推移を追う。これにより、患者毎の多種多様なバックグラウンドや反応個体差に捉われずに、病態推移や薬剤副作用と深く関連するメカニズムや細胞などを見出せると期待する。本研究では、何らかの機能既知の分子に着目した測定や解析から始めるのではなく、網羅的なパラメータの取得によりプロファイリングすることで病態や治療と関連する因子を探索する。その後、見出された分子の変動確認や機能探索などを通じ、病態理解と治療法の開発に繋げる。","en":"In order to grasp the biological reactions and pathological conditions occurring in the body in more detail for diseases involving immune reactions, blood and tissue samples were used and analyzed by following the changes and changes over time for each patient. The present research will acquire and profile comprehensive parameters for each patient and explore factors related to disease conditions and treatments. Then, it will be linked to the understanding of pathology and the development of therapeutic methods through the confirmation of changes in the found molecules and the search for functions."},"methods":{"ja":"single cell CITE-seq（Cellular indexing of transcriptomes and epitopes）、BD Ab-seq、single cell RNA-seq、シングルセル空間トランスクリプトーム","en":"single-cell CITE-seq (Cellular indexing of transcriptomes and epitopes), BD Ab-seq, single-cell RNA-seq"},"targets":{"ja":"顕微鏡的多発血管炎（microscopic polyangiitis：MPA）、全身性強皮症（Systemic sclerosis : SSc）、シェーグレン症候群（Sjogren Syndrome：SjS） 、ベーチェット症候群（Behcet's Syndrome：BS）、 健常者","en":"microscopic polyangiitis (MPA), systemic sclerosis (SSc), Sjögren's Syndrome (SjS), Behçet's Syndrome (BS), healthy donors"},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"・全身性強皮症21症例および健常者6名の末梢血から取得したPBMCを単細胞へ分離した上でmRNAを抽出し、CITE-seq解析を実施した。fastqデータならびに発現マトリクスデータ（HDF5）を提供する。\n・全身性強皮症1症例の末梢血より取得した全白血球、腎生検検体より取得した全細胞からmRNAを抽出し、BD Ab-seq解析を実施した。fastqデータならびに発現マトリクスデータ（txt）を提供する。","en":"- RNA extracted from PBMCs collected from 21 patients with systemic sclerosis (SSc) and 6 healthy donors were used for CITE-seq analysis. The resulting fastq data and expression matrix data (HDF5) are provided.\n- RNA extracted from whole blood leukocytes and kidney biopsy specimens collected from a SSc patient with renal crisis were used for BD Ab-seq analysis. The resulting fastq data and expression matrix data (txt) are provided."},"dataProviders":[{"name":{"ja":"北沢 剛久","en":"Takehisa Kitazawa"},"organization":{"name":{"ja":"中外製薬株式会社 研究本部","en":"Research Division, Chugai Pharmaceutical Co., Ltd."}}}],"researchProjects":[],"grants":[],"relatedPublications":[{"title":"Single-cell multi-omics analysis identifies two distinct phenotypes of newly-onset microscopic polyangiitis","doi":"https://doi.org/10.1038/s41467-023-41328-0","datasets":["E-GEAD-635"]},{"title":"Neutrophil single-cell analysis identifies a type II interferon-related subset for predicting relapse of autoimmune small vessel vasculitis","doi":"https://doi.org/10.1038/s41467-025-58550-7","datasets":["DRA019233","E-GEAD-867"]},{"title":"Single-cell analysis reveals immune cell abnormalities underlying the clinical heterogeneity of systemic sclerosis","doi":"https://doi.org/10.21203/rs.3.rs-4728677/v1","datasets":["DRA019274","E-GEAD-872"]},{"title":"Deciphering the Single-Cell Spatiotemporal Landscape of Tertiary Lymphoid Structures in Primary Sjögren's Syndrome","doi":null,"datasets":["DRA020399","E-GEAD-1051","E-GEAD-1057"]},{"title":"Pathological Involvement of the Classical Complement Pathway and Neutrophil Activation in Behçet's Syndrome Revealed by Single-Cell Transcriptomics and Serum Active C1s","doi":null,"datasets":["DRA021372","E-GEAD-1076"]}],"datasets":["DRA019274","E-GEAD-635","E-GEAD-872","DRA019233","E-GEAD-867"],"controlledAccessUsers":[]}