{"id":"hum0405","version":4,"url":"https://humandbs.dbcls.jp/research/hum0405/v4","datePublished":"2026-08-05","versions":[{"version":1,"datePublished":"2023-12-25"},{"version":2,"datePublished":"2025-07-16"},{"version":3,"datePublished":"2025-12-02"},{"version":4,"datePublished":"2026-08-05"}],"title":{"ja":"小児・成人悪性腫瘍がん幹細胞の同定に関する研究","en":"Research on the identification of cancer stem cells for peidatric and adult malignancies"},"summary":{"aims":{"ja":"近年、白血病・脳腫瘍・乳がん等多くのがんにおいて「がん幹細胞」が存在することが報告され、がんの再発の主原因であることが多くの報告で明らかにされてきているが、多くの小児・成人悪性腫瘍ではがん幹細胞を明確に同定し、その生物学的特性を検討した報告は存在しない。本研究では小児・成人悪性腫瘍の患者検体を使用して、がん幹細胞を同定が可能であるかを検証することを目的とする。希少な小児がんに対し、体細胞・胚細胞変異を包括したゲノミクスや、トランスクリプトミクス、プロテオミクス、メタボロミクス などを統合したオミクス解析を展開することで、がん幹細胞や前駆細胞レベルでの病態、個体間差異を含めた腫瘍を取り巻く環境などの対象疾患の生物学的特性の理解を深める。得られた知見と患者臨床情報を元に、マウスモデルやin vitroモデルを用いて新規治療薬や細胞療法など新規治療戦略による非臨床薬理試験を行い、治療効果を検討する。","en":"In recent years, \"cancer stem cells\" have been reported to exist in many cancers. Many reports have shown that they are the main cause of cancer recurrence. The aim of this study is to test whether cancer stem cells can be identified using patient samples. We will perform omics analysis of rare pediatric cancers by integrating genomics, transcriptomics, proteomics and metabolomics, including somatic and germline mutations, to deepen our understanding of the biological characteristics of the target disease. Based on the knowledge gained and clinical information from patients, we will conduct preclinical pharmacological studies using mouse models and in vitro models to investigate the therapeutic effects of novel therapeutic agents and novel therapeutic strategies such as cellular therapies."},"methods":{"ja":"WES、RNA-seq、small RNA-seq、WGS、Amplicon-seq、メチル化アレイ、scRNA-seq/ADT-seq/TCR-seq、CUT&RUN-seq","en":"WES, RNA-seq, small RNA-seq, WGS, Amplicon-seq, Methylation array, scRNA-seq/ADT-seq/TCR-seq, CUT&RUN-seq"},"targets":{"ja":"小児B前駆細胞性急性リンパ性白血病 69症例\n小児急性骨髄性白血病 9症例（内、5症例は初発時は小児B前駆細胞性急性リンパ性白血病、再発時に急性骨髄性白血病を呈した症例）\nCD19 CAR-T療法（tisagenlecleucel）を受けたB細胞性前リンパ球性白血病（B-cell precursor ALL：BCP-ALL）16症例\n慢性活動性ウイルス感染症（chronic active EBV infection：CAEBV） 65症例\n伝染性単核球症（infectious mononucleosis：IM） 6症例\n健常人 4名","en":"Pediatric B-cell precursor acute lymphocytic leukemia: 69 cases\nPediatric acute myeloid leukemia: 9 cases\nB-cell precursor acute lymphocytic leukemia patients who received tisagenlecleucel: 16 cases\nchronic active EBV infection (CAEBV): 65 cases\ninfectious mononucleosis (IM): 6 cases\n4 healthy volunteers"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\n配列決定\nメチル化\nヒストン修飾","en":"Expression profiling\nSequencing\nMethylation profiling\nHistone modification"},"targets":{"ja":"小児B前駆細胞性急性リンパ性白血病：69症例\n小児急性骨髄性白血病：9症例\nB細胞性前リンパ球性白血病：16症例\n慢性活動性EBウイルス感染症：65症例\n伝染性単核球症：6症例\n健常人：4名\n（日本人）","en":"Pediatric B-cell precursor acute lymphocytic leukemia: 69 cases\nPediatric acute myeloid leukemia: 9 cases\nB-cell precursor acute lymphoblastic leukemia: 16 cases\nchronic active EBV infection: 65 cases\ninfectious mononucleosis: 6 cases\n4 healthy individuals\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq、small RNA-seq、WGS、Amplicon-seq、scRNA-seq/ADT-seq/TCR-seq、CUT&RUN-seq）\nメチル化アレイ","en":"NGS\n(Exome, RNA-seq, small RNA-seq, WGS, Amplicon-seq, scRNA-seq/ADT-seq/TCR-seq, CUT&RUN-seq)\nMethylation array"}},"releaseNote":{"ja":"慢性活動性EBV感染症（CAEBV）65症例のEBV感染細胞もしくは細胞株、非感染細胞、末梢血単核球（PBMC）、ならびに、伝染性単核球症（IM）6症例、健常人4名のNK細胞またはPBMCより抽出したDNA/RNAを用いたWGS、WES、RNA-seq、Amplicon-seq、メチル化アレイ、scRNA-seq/ADT-seq/TCR-seq、およびCUT&RUN-seq解析データをbam、bai、fastq、IDATファイルにて提供する。","en":"DNAs/RNAs were extracted from EBV-infected cells, cell-lines, uninfected cells and PBMCs from 65 patiants with chronic active EBV infection (CAEBV), NK cells and PBMCs from 6 patiants with infectious mononucleosis (IM) and 4 healthy individuals. WGS, WES, Amplicon-seq, RNA-seq, Methylation array, scRNA-seq/ADT-seq/TCR-seq and CUT&RUN-seq analyses were performed. Bam, bai, IDAT and fastq files are provided."},"dataProviders":[{"name":{"ja":"滝田 順子","en":"Junko Takita"},"organization":{"name":{"ja":"京都大学 発達小児科学","en":"Department of Pediatrics, Graduate School of Medicine, Kyoto University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"難治性小児がんの時空的多様性の解明と新規創薬の開発","en":"Study of spatiotemporal variety of intractable pediatric cancers and development of new drugs"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療加速化研究事業（P-PROMOTE）","en":"Project for Promotion of Cancer Research and Therapeutic Evolution (P-PROMOTE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP22ama221505"]},{"title":{"ja":"マルチオミックス情報を基盤とした難治性小児がんに対する新規克服法の開発","en":"Development of novel therapeutic strategies for intractable pediatric cancers based on the multi-omics information"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["17H04224"]},{"title":{"ja":"小児がんにおける遺伝学的高発がん感受性の機序とクローン進化の統合的解析","en":"Integrated analysis of mechanisms of genetic susceptibility to cancer and clonal evolution in pediatric cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（A）","en":"KAKENHI Grant-in-Aid for Scientific Research (A)"},"grantIds":["20H00528"]},{"title":{"ja":"小児固形腫瘍の克服に資するドライバー遺伝子を標的としない新規治療法の開発","en":"Development of novel therapeutic strategies for pediatric solid tumor based on non-driver gene targeted approaches"},"agency":{"ja":"科学研究費助成事業 挑戦的研究（萌芽）","en":"KAKENHI Grant-in-Aid for Challenging Research (Exploratory)"},"grantIds":["21K19405"]},{"title":{"ja":"神経発達症群と腫瘍形成におけるクロストークの解明と新規創薬の開発","en":"Elucidation of crosstalk between neurodevelopmental disorders and tumorigenesis and development of novel therapeutic drugs"},"agency":{"ja":"科学研究費助成事業 挑戦的研究（萌芽）","en":"KAKENHI Grant-in-Aid for Challenging Research (Exploratory)"},"grantIds":["23K18264"]},{"title":{"ja":"小児期から若年成人期に発症する悪性腫瘍の克服に資する時空的多様性の解明","en":"Clarification of spatio-temporal diversity for overcoming cancers that develop from childhood to young adulthood"},"agency":{"ja":"科学研究費助成事業 基盤研究（A）","en":"KAKENHI Grant-in-Aid for Scientific Research (A)"},"grantIds":["24H00628"]}],"relatedPublications":[{"title":"RNA-seq-based miRNA signature as an independent predictor of relapse in pediatric B-cell acute lymphoblastic leukemia","doi":"https://doi.org/10.1182/bloodadvances.2023011583","datasets":["JGAD000752"]},{"title":"Multi-omics analysis identifies an M-MDSC-like immunosuppressive phenotype in lineage-switched AML with KMT2A rearrangement","doi":"https://doi.org/10.1038/s41467-025-63271-y","datasets":["JGAD000761"]},{"title":"CAR-T cells with the CD38-CD73-Tim-3-HLA-DR+ phenotype predict the efficacy of tisagenlecleucel as a treatment for B cell precursor ALL","doi":"https://doi.org/10.1016/j.xcrm.2025.102576","datasets":["JGAD000901"]},{"title":"Multiomics analysis reveals the genetic and epigenetic features of high-risk NK cell-type chronic active EBV infection","doi":"https://doi.org/10.1182/blood.2024026805","datasets":["JGAD000870"]}],"datasets":["JGAD000752","JGAD000761","JGAD000901","JGAD000870"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000752","JGAD000761","JGAD000901"]}]}