{"id":"hum0403","version":1,"url":"https://humandbs.dbcls.jp/research/hum0403/v1","datePublished":"2023-07-27","versions":[{"version":1,"datePublished":"2023-07-27"}],"title":{"ja":"泌尿器癌におけるリキッドバイオプシーの有用性の検討","en":"Investigation of the utility of liquid biopsy in urologic cancer"},"summary":{"aims":{"ja":"泌尿器がん症例から採取された腫瘍組織と遊離核酸を用い、遺伝子変異およびmicro RNA発現を評価し、新たなバイオマーカーの同定を前向き研究として行うこと。","en":"To prospectively identify new biomarkers by evaluating gene mutations and microRNA expression using tumor tissues and free nucleic acids collected from patients with urologic cancer."},"methods":{"ja":"抽出した血漿のセルフリーDNAの濃度は、Qubit fluorometer（Thermo Fisher Scientific, Waltham, MA, USA）を用いて評し、AVENIO ctDNA Expanded Kit（Roche Diagnostics, Basel, Switzerland）を用いて循環腫瘍DNA解析を行った。","en":"Cell-free DNAs extracted from plasma samples were subjected to a circulating tumor DNA analysis using the AVENIO ctDNA Expanded Kit (Roche Diagnostics, Basel, Switzerland). DNA concentration was assessed using a Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA, USA)."},"targets":{"ja":"転移性尿路上皮がん16症例から採取した免疫チェックポイント阻害剤投与前の血漿16検体と投与後の血漿9検体（計25検体）","en":"16 plasma samples were collected before immune checkpoint inhibitor treatment and 9 plasma samples were collected after immune checkpoint inhibitor treatment from 16 metastatic urothelial carcinoma patients."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"転移性尿路上皮癌：16症例\n（日本人）","en":"Metastatic urothelial carcinoma: 16 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Target Capture）","en":"NGS\n(Target Capture)"}},"releaseNote":{"ja":"転移性尿路上皮がん症例の免疫チェックポイント阻害剤投与前後の血漿中セルフリーDNAを用いた77遺伝子のTarget Capture Sequencing解析データをbamファイルにて提供する。","en":"Cell-free DNAs extracted from plasma samples collected before and/or after immune checkpoint inhibitor treatment from metastatic urothelial carcinoma patients were used for the target capture sequencing analysis. Bam files are provided."},"dataProviders":[{"name":{"ja":"濱田 和希","en":"Kazuki Hamada"},"organization":{"name":{"ja":"筑波大学附属病院 腎泌尿器外科","en":"Department of Urology, University of Tsukuba Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"泌尿器癌における全血RNAを用いた免疫細胞の多様性の理解と治療効果予測への応用","en":"Application of whole blood RNA in urological cancer to understand immune cell diversity and predict therapeutic effect"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["21K09365"]}],"relatedPublications":[{"title":"Variant allele frequency changes in TP53 predict pembrolizumab response in patients with metastatic urothelial carcinoma.","doi":"https://doi.org/10.3892/ol.2023.13975","datasets":["JGAD000751"]}],"datasets":["JGAD000751"],"controlledAccessUsers":[]}