{"id":"hum0393","secondaryIds":[],"version":1,"url":"https://humandbs.dbcls.jp/research/hum0393/v1","datePublished":"2024-08-23","versions":[{"version":1,"datePublished":"2024-08-23"},{"version":2,"datePublished":"2025-10-28"}],"title":{"ja":"膠原病類縁疾患患者検体を用いた新規バイオマーカーおよび治療標的分子探索に関する研究","en":"To explore new biomarkers or therapeutic target molecules using samples with patients with autoimmune diseases"},"summary":{"aims":{"ja":"膠原病類縁疾患の新規バイオマーカーおよび治療標的分子の探索","en":"To explore new biomarkers or therapeutic target molecules using samples from patients with autoimmune diseases"},"methods":{"ja":"Moflo XDPセルソーター （Beckman Coulter）によりCD4+CD45RA-CD25hiCD127lo Treg細胞を分取後、TRIZOL-LS（ThermoFisher/Invitrogen）にて抽出したRNAを用いたRNA-seq解析","en":"CD4+CD45RA-CD25hiCD127lo Treg cells were sorted on Moflo XDP cell sorter (Beckman Coulter) from each peripheral blood sample. Total RNA was extracted with TRIZOL-LS (ThermoFisher/Invitrogen) for RNA-seq."},"targets":{"ja":"未治療の関節リウマチ6症例、対照健常者6名","en":"Twelve peripheral blood samples were collected from six treatment-naïve rheumatoid arthritis (RA) patients and six healthy individuals."},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"関節リウマチ6症例および健常者6名の末梢血より分取したCD4+CD45RA-CD25hiCD127lo Treg細胞から抽出したRNAを用いたRNA-seq解析データおよび発現量（TPM）をmatrixにまとめたデータをfastq、csvファイルにて提供する。"},"dataProviders":[{"name":{"ja":"坪井 洋人","en":"Hiroto Tsuboi"},"organization":{"name":{"ja":"筑波大学 医学医療系 膠原病リウマチアレルギー内科学","en":"Department of Rheumatology, University of Tsukuba"}}}],"researchProjects":[],"grants":[{"title":{"ja":"関節リウマチにおける濾胞性制御性T細胞の機能と疲弊の解明","en":"Mechanisms and exhaustion of T follicular regulatory cells in rheumatoid arthritis"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["21H02959"]},{"title":{"ja":"関節リウマチの加齢に伴う制御性T細胞の機能変化解析","en":"Analysis of age-related changes in regulatory T cell function in rheumatoid arthritis"},"agency":{"ja":"JST科学技術イノベーション創出に向けた大学フェローシップ創設事業","en":"establishment of university fellowships towards the creation of science technology innovation, JST"},"grantIds":["JPMJFS2106"]}],"relatedPublications":[{"title":"Mechanisms of age-related Treg dysfunction in an arthritic environment","doi":"https://doi.org/10.1016/j.clim.2024.110337","datasets":["JGAD000826"]}],"datasets":["JGAD000826"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000760","JGAD000826"]},{"principalInvestigator":{"ja":"上原 剛","en":"Takeshi Uehara"},"affiliation":{"ja":"医学部病態解析診断学 、医学部附属病院病理診断科・臨床検査部, 信州大学","en":"Department of Laboratory Medicine Department of Diagnostic Pathology, Shinshu university"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"IgG4関連疾患および類似疾患における分子発現プロファイルの統合解析\r\n― 公開トランスクリプトームデータおよび自施設病理検体を用いた検討 ―","en":"Integrated analysis of molecular expression profiles in IgG4-related diseases and related conditions"},"periodStart":"2026-02-26","periodEnd":"2031-01-31","datasets":["JGAD000760"]}]}