{"id":"hum0393","version":2,"url":"https://humandbs.dbcls.jp/research/hum0393/v2","datePublished":"2025-10-28","versions":[{"version":1,"datePublished":"2024-08-23"},{"version":2,"datePublished":"2025-10-28"}],"title":{"ja":"膠原病類縁疾患患者検体を用いた新規バイオマーカーおよび治療標的分子探索に関する研究","en":"To explore new biomarkers or therapeutic target molecules using samples with patients with autoimmune diseases"},"summary":{"aims":{"ja":"膠原病類縁疾患の新規バイオマーカーおよび治療標的分子の探索","en":"To explore new biomarkers or therapeutic target molecules using samples from patients with autoimmune diseases"},"methods":{"ja":"JGAS000693：Moflo XDPセルソーター（Beckman Coulter）によりCD4+CD45RA-CD25hiCD127lo Treg細胞を分取後、TRIZOL-LS（ThermoFisher/Invitrogen）にて抽出したRNAを用いたRNA-seq解析\nJGAS000630：human CD19 MicroBeads（Miltenyi Biotec）およびhuman PanT Cell Isolation Kit（Miltenyi Biotec）を用いてCD19+細胞およびT細胞を回収し、TRIZOL-LS（ThermoFisher/Invitrogen）にて抽出したRNAを用いたRNA-seq解析","en":"JGAS000693: CD4+CD45RA-CD25hiCD127lo Treg cells were sorted on Moflo XDP cell sorter (Beckman Coulter) from each peripheral blood sample. Total RNA was extracted with TRIZOL-LS (ThermoFisher/Invitrogen) for RNA-seq.\nJGAS000630: CD19+cells were separated by magnetic-activated cell sorting (MACS) using human CD19 MicroBeads (Miltenyi Biotec), and then PanT cells were also sorted using human PanT Cell Isolation Kit (Miltenyi Biotec) from each sample. Total RNA was extracted with TRIZOL-LS (ThermoFisher/Invitrogen) for RNA-seq."},"targets":{"ja":"JGAS000693：未治療の関節リウマチ6症例、対照健常者6名\nJGAS000630：未治療のIgG4関連疾患4症例、アメリカリウマチ学会/ヨーロッパリウマチ学会の基準を満たす原発性シェーグレン症候群3症例および対照健常者3名","en":"JGAS000693: Twelve peripheral blood samples were collected from six treatment-naïve rheumatoid arthritis (RA) patients and six healthy individuals.\nJGAS000630: Submandibular glands (SMGs) (N=3) and peripheral blood mononuclear cells (PBMCs) (N=4) were collected from four patients with treatment naïve IgG4 related diseases (IgG4-RD). PBMCs were also collected from three patients with primary Sjögren's syndrome (pSS) who fulfilled American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) Classification Criteria for pSS, and three healthy controls."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"関節リウマチ：6症例\n健常者：6名\nIgG4関連疾患：4症例\n原発生シェーグレン症候群：3症例\n健常者：3名\n（日本人）","en":"treatment-naïve rheumatoid arthritis: 6 cases\n6 healthy individuals\ntreatment naïve IgG4-RD: 4 cases\nprimary Sjögren's syndrome: 3 cases\n3 healthy individuals\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"未治療のIgG4関連疾患4症例ならびに、アメリカリウマチ学会/ヨーロッパリウマチ学会の基準を満たす原発性シェーグレン症候群3症例および健常者3名のCD19+細胞およびT細胞から抽出したRNAを用いたRNA-seq解析データをfastqファイルにて提供する。","en":"RNA extracted from CD19+ cells and T cells of 4 cases of treatment-naïve IgG4-related disease, 3 cases of primary Sjögren's syndrome meeting the ACR/EULAR classification criteria, and 3 healthy individuals were used for RNA-seq analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"坪井 洋人","en":"Hiroto Tsuboi"},"organization":{"name":{"ja":"筑波大学 医学医療系 膠原病リウマチアレルギー内科学","en":"Department of Rheumatology, University of Tsukuba"}}}],"researchProjects":[],"grants":[{"title":{"ja":"関節リウマチにおける濾胞性制御性T細胞の機能と疲弊の解明","en":"Mechanisms and exhaustion of T follicular regulatory cells in rheumatoid arthritis"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["21H02959"]},{"title":{"ja":"関節リウマチの加齢に伴う制御性T細胞の機能変化解析","en":"Analysis of age-related changes in regulatory T cell function in rheumatoid arthritis"},"agency":{"ja":"JST科学技術イノベーション創出に向けた大学フェローシップ創設事業","en":"establishment of university fellowships towards the creation of science technology innovation, JST"},"grantIds":["JPMJFS2106"]},{"title":{"ja":"IgG4関連疾患におけるRNA-Seqを用いた遺伝子発現解析と新規治療標的の開発","en":"Gene expression analysis by RNA-Seq and development of novel therapeutic target in IgG4-related disease"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["21K08471"]}],"relatedPublications":[{"title":"Mechanisms of age-related Treg dysfunction in an arthritic environment","doi":"https://doi.org/10.1016/j.clim.2024.110337","datasets":["JGAD000826"]},{"title":"Analysis of lymphocytes specific gene expression pattern by RNA-Seq in patients with IgG4-related disease: Comparison between submandibular glands and peripheral blood","doi":"https://doi.org/10.1093/mr/roaf059","datasets":["JGAD000760"]}],"datasets":["JGAD000826","JGAD000760"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000760","JGAD000826"]},{"principalInvestigator":{"ja":"上原 剛","en":"Takeshi Uehara"},"affiliation":{"ja":"医学部病態解析診断学 、医学部附属病院病理診断科・臨床検査部, 信州大学","en":"Department of Laboratory Medicine Department of Diagnostic Pathology, Shinshu university"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"IgG4関連疾患および類似疾患における分子発現プロファイルの統合解析\r\n― 公開トランスクリプトームデータおよび自施設病理検体を用いた検討 ―","en":"Integrated analysis of molecular expression profiles in IgG4-related diseases and related conditions"},"periodStart":"2026-02-26","periodEnd":"2031-01-31","datasets":["JGAD000760"]}]}