{"id":"hum0391","version":1,"url":"https://humandbs.dbcls.jp/research/hum0391/v1","datePublished":"2024-09-25","versions":[{"version":1,"datePublished":"2024-09-25"}],"title":{"ja":"ヒト舌癌オルガノイドバイオバンクの構築","en":"Establishment of a human tongue cancer organoid biobank"},"summary":{"aims":{"ja":"化学療法剤を用いたがん治療では、化学療法耐性のわずかながん細胞が生き残り、がん再発の原因となる。したがって、この残存がん細胞が生じる分子基盤を解明し、それらを駆逐する手法を確立することは、多くのがん患者の究明につながる。本研究では、独自に樹立したヒト舌がんオルガノイドライブラリーを対象に、1細胞マルチオーム解析を用いて、化学療法耐性がんクローンの性質を遺伝子発現およびクロマチンアクセシビリティーの特徴から明らかにし、それらを駆逐するための分子標的を探索する。","en":"In cancer treatment using chemotherapeutic agents, a few chemotherapy-resistant cancer cells survive and cause cancer recurrence. These cancer cell subpopulations with anticancer drug-resistant properties called \"persister\" tumor cells. Therefore, elucidating the molecular basis of persistent tumor generation and establishing eradication strategies of them will aid of many cancer patients. In this study, we use single-cell Multiome analysis (10X Genomics) of a uniquely established human tongue cancer organoid library to characterize persister tumor cell properties based on their gene expression profile and chromatin accessibility."},"methods":{"ja":"scMultiome（10x Genomics）解析","en":"scMultiome (10x Genomics) analysis"},"targets":{"ja":"舌がん症例の腫瘍組織から樹立した舌がんオルガノイド","en":"Tongue cancer organoids established from tumor tissues of tongue cancer patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\nクロマチン構造","en":"Expression profiling, Chromatin structure profiling"},"targets":{"ja":"舌癌：4症例\n（日本人）","en":"Tongue cancer: 4 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq、scATAC-seq）","en":"NGS\n(scRNA-seq, scATAC-seq)"}},"releaseNote":{"ja":"舌がん4症例の腫瘍組織から樹立した舌がんオルガノイド4検体から抽出したDNA/RNAを用いたsingle-cell Multiome（scRNA+scATAC）解析データをfastqファイルにて提供する。","en":"DNAs/RNAs extracted from tongue cancer organoids established from tumor tissues of tongue cancer patients are used to scMultiome (scRNA+scATAC) analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"佐藤 卓","en":"Taku Sato"},"organization":{"name":{"ja":"東京医科歯科大学 難治疾患研究所 生体防御学分野 / 日本医科大学 大学院医学研究科 代謝・栄養学分野","en":"Department of Biodefence Research, Medical Research Institute, Tokyo Medical and Dental University / Department of Biochemistry and Molecular Biology, Nippon Medical School Graduate School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Sciences"},"agency":{"ja":"科学研究費助成事業 学術変革領域研究","en":"KAKENHI Grant-in-Aid for Transformative Research Areas"},"grantIds":["22H04925"]}],"relatedPublications":[{"title":"Comparative analysis of organoids derived from tongue cancer patients identifies the heritable nature of minimal residual disease","doi":"https://doi.org/10.1016/j.devcel.2024.10.007","datasets":["JGAD000735"]}],"datasets":["JGAD000735"],"controlledAccessUsers":[]}