{"id":"hum0383","version":1,"url":"https://humandbs.dbcls.jp/research/hum0383/v1","datePublished":"2023-03-28","versions":[{"version":1,"datePublished":"2023-03-28"}],"title":{"ja":"生物学的製剤投与前後のリンパ球の遺伝子発現修飾の解析","en":"Analysis of the effect of abatacept for the transcriptome of multiple lymphoid subsets"},"summary":{"aims":{"ja":"関節リウマチの治療においてT細胞の共刺激を阻害するCTLA4-Igであるアバタセプトの作用点及び予後予測因子の解明","en":"To elucidate the mechanism and predictors of abatacept, a CTLA4 agonist that regulates T cell co-stimulation, in rheumatoid arthritis (RA) treatment."},"methods":{"ja":"関節リウマチ症例に対するアバタセプト治療前後の末梢血由来の免疫細胞18画分を分取し（CD16p_Mono, CL_Mono, DN_B, Fr_II_eTreg, mDC, Mem_CD4, Naive_B, Naive_CD4, Neu, NK, pDC, Plasmablast, SM_B, Tfh, Th1, Th17, Th2, USM_B）、各画分のRNA-seqを実施した。","en":"Various peripheral blood immune cell subsets from 5 RA patients were collected (CD16p_Mono, CL_Mono, DN_B, Fr_II_eTreg, mDC, Mem_CD4, Naive_B, Naive_CD4, Neu, NK, pDC, Plasmablast, SM_B, Tfh, Th1, Th17, Th2, USM_B). The patients were analyzed again longitudinally after abatacept treatment. RNA-seq was performed with each immune cell subset sample."},"targets":{"ja":"関節リウマチ5症例","en":"5 RA patients"},"url":{"ja":[{"url":"https://ryumachi.umin.jp/","text":"https://ryumachi.umin.jp/"}],"en":[{"url":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html","text":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"関節リウマチ：5症例\n（日本人）","en":"Rheumatoid arthritis: 5 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"関節リウマチ5症例の末梢血免疫細胞18画分を分取し、各画分サンプルを用いたRNA-seqを実施した。サンプルのフィルタリングの上で各遺伝子にマップされたリード数を提供する（txt）。","en":"18 immune cell subsets from 5 rheumatoid arthritis patients were collected. RNA-seq was performed with each immune cell subset sample. After filtering of the gene expression data, read count data mapped to each gene are provided as txt files."},"dataProviders":[{"name":{"ja":"藤尾 圭志","en":"Keishi Fujio"},"organization":{"name":{"ja":"東京大学大学院 医学系研究科 アレルギー・リウマチ学","en":"Department of Allergy and Rheumatology, Graduate School of Medicine, The University of Tokyo"}}}],"researchProjects":[{"name":{"ja":"生物学的製剤投与前後のリンパ球の遺伝子発現修飾の解析","en":"Analysis of the effect of abatacept for the transcriptome of multiple lymphoid subsets"},"url":{"ja":[{"url":"https://ryumachi.umin.jp/","text":"https://ryumachi.umin.jp/"}],"en":[{"url":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html","text":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html"}]}}],"grants":[{"title":{"ja":"生物学的製剤投与前後のリンパ球の遺伝子発現修飾の解析","en":"Analysis of the effect of abatacept for the transcriptome of multiple lymphoid subsets"},"agency":{"ja":"ブリストルマイヤーズスクイブ株式会社との共同研究費","en":"Collaborative research fund with Bristol Myers Squibb Japan"},"grantIds":null}],"relatedPublications":[{"title":"Immunomics analysis of rheumatoid arthritis identified precursor dendritic cells as a key cell subset of treatment resistance","doi":"https://doi.org/10.1136/ard-2022-223645","datasets":["JGAD000731","JGAD000727"]}],"datasets":["JGAD000731"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000731"]},{"principalInvestigator":{"ja":"久保田 晋平","en":"Shimpei Kubota"},"affiliation":{"ja":"遺伝子病制御研究所, 北海道大学","en":"Molecular Psychoneuroimmunology, Hokkaido University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"関節リウマチおよび全身性エリテマトーデスにおける遺伝子発現および炎症増幅回路活性化因子の解析","en":"Gene expression and IL-6 amplifying circuit activators in rheumatoid arthritis and systemic lupus erythematosus"},"periodStart":"2024-02-26","periodEnd":"2026-03-31","datasets":["JGAD000731"]},{"principalInvestigator":{"en":"Pranidhi Sood"},"affiliation":{"en":"Wield Therapeutics, Inc"},"country":{"ja":"アメリカ合衆国 (カリフォルニア州)","en":"California, United States"},"researchTitle":{"en":"Identifying Gene Expression Based Biomarkers in Patients with Autoimmune Diseases to Predict\r\nResponse to New Therapies Developed by Wield Therapeutics"},"periodStart":"2025-06-25","periodEnd":"2027-04-23","datasets":["JGAD000731"]}]}