{"id":"hum0376","version":2,"url":"https://humandbs.dbcls.jp/research/hum0376/v2","datePublished":"2023-03-28","versions":[{"version":1,"datePublished":"2022-12-27"},{"version":2,"datePublished":"2023-03-28"}],"title":{"ja":"臨床検体からのゼノグラフト株・培養細胞株の樹立","en":"Establishment of xenografts and cultured cell lines from clinical samples."},"summary":{"aims":{"ja":"治療法の開発に資する研究リソースとして、臨床検体からゼノグラフト株・培養細胞株を樹立すること。ならびに、樹立方法を開発すること。","en":"To establish xenografts and cultured cell lines from clinical samples, as a bioresource for the development of a method of treatment, and to investigate their establishment methods."},"methods":{"ja":"子宮内膜がん症例の腫瘍細胞および腫瘍細胞から樹立したゼノグラフト株から抽出したDNAを用いたNCCオンコパネル v4を用いて114遺伝子の変異および12遺伝子の増幅と融合の有無を解析した。","en":"A targeted sequencing analysis was performed using the NCC Oncopanel v4 test, which can analyze mutations of 114 genes and amplifications and fusions of 12 genes."},"targets":{"ja":"子宮内膜がん2＋4症例の腫瘍組織とゼノグラフト株から各1検体ずつ","en":"Endometrial cancer samples and their xenografts. Samples of one parent tissue and one xenograft each from two + four cases."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"子宮内膜癌：2＋4症例\n（日本人）","en":"endometrial cancer: 2 + 4 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Target Capture）","en":"NGS\n(Target Capture)"}},"releaseNote":{"ja":"子宮内膜がん4症例の腫瘍組織および腫瘍組織から樹立したゼノグラフト株から抽出したDNAを用いたTarget Capture Sequencing解析データ（NCC Oncopanel v4）をbamファイルにて提供する。","en":"DNAs extracted from tumor tissues from patients with endometrial cancer and patient-derived xenograft samples were used for the target capture sequencing analysis (NCC Oncopanel v4). Bam files are provided."},"dataProviders":[{"name":{"ja":"今井 俊夫","en":"Toshio Imai"},"organization":{"name":{"ja":"国立がん研究センター研究所 がんモデル開発部門","en":"Department of Cancer Model Development, National Cancer Center Research Institute"}}}],"researchProjects":[],"grants":[{"title":{"ja":"FIOCのコアファシリティ機能とバイオリソースの進化・結合によるTR・創薬研究推進","en":"Promotion of TR and drug discovery research by evolving and combining FIOC fore facility functions and bioresources"},"agency":{"ja":"国立がん研究センター研究開発費","en":"The National Cancer Center Research and Development Fund"},"grantIds":["2020J-002"]}],"relatedPublications":[{"title":"Alteration in molecular properties during establishment and passaging of endometrial carcinoma patient-derived xenografts","doi":"https://doi.org/10.1038/s41598-023-35703-6","datasets":["JGAD000712"]}],"datasets":["JGAD000712"],"controlledAccessUsers":[]}