{"id":"hum0371","version":1,"url":"https://humandbs.dbcls.jp/research/hum0371/v1","datePublished":"2022-12-22","versions":[{"version":1,"datePublished":"2022-12-22"}],"title":{"ja":"肝臓疾患に関与する遺伝子とタンパク質の探索に関する研究","en":"Research on genes and proteins associated with liver disease."},"summary":{"aims":{"ja":"慢性肝炎、肝硬変、肝癌など肝臓病は国民の健康に多大な影響を与えている。我が国の肝臓病の大半はB型肝炎ウイルス、C型肝炎ウイルス等の肝炎ウイルス感染症で、ウイルス遺伝子の違いによって肝炎の病態が異なり、抗ウイルス療法の治療効果にもウイルス遺伝子が影響する。一方、宿主の遺伝子やその発現も病態に関与している。ポストゲノム時代の現在ではゲノム解析、トランスクリプトーム解析が行われる一方、質量分析によるプロテオーム研究も重要になっている。また、慢性肝炎や脂肪肝の病態と腸内細菌の関連が明らかになり、薬剤奏効性との関連性も示唆されている。本研究の目的は、これらのDNA、RNA、蛋白質に関する網羅的・包括的解析の手法を用い、ウイルス側、宿主側の両面から、肝臓疾患の診断と病態把握に役立つ遺伝子関連情報を得て日常診療に役立てる、更には疾患の早期発見、治療後の予後予測に結び付けられる方法を開発することである。","en":"Liver diseases, including chronic hepatitis, cirrhosis, and liver cancer, are prevalent in Japan, which are often caused by hepatitis viral infections. The pathogenesis of hepatitis and the selection of antiviral therapies varies depending on the genotypes or sequences of the viruses. Genes and gene expression levels of the host are also involved in the pathogenesis. Recently, along with genomics and transcriptomics, proteomics and metagenomics are also available which have revealed that gut flora is also associated with the pathogenesis and therapeutic efficacy of drugs in chronic hepatitis. The aim of this study is to establish a comprehensive genetic diagnosis system for chronic liver diseases by investigating genes and proteins associated with chronic liver diseases from both the viral and host side."},"methods":{"ja":"16Sメタゲノミクス解析","en":"16S metagenomic analysis was performed on stool DNA samples collected from 27 patients who experienced liver biopsy according to the following criteria or clinically diagnosed severe non-B non-C cirrhosis at the University of Tokyo Hospital. After excluding patients who had never been diagnosed with diabetes (6 cases) and patients who were categorized to Matteoni classification class 2 or 1 (1 case), 20 patients with diabetes who were diagnosed NASH as Matteoni classification class 4 or clinically diagnosed liver cirrhosis were analyzed. The 16S metagenomic data from 5 patients who were under insulin treatment were compared with those of the remaining 15 patients."},"targets":{"ja":"糖尿病を併発する非アルコール性脂肪性肝炎（マッテオーニ分類3もしくは4と病理診断された）20症例","en":"20 patients with nonalcoholic steatohepatitis (NASH)"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"メタゲノム","en":"Metagenome"},"targets":{"ja":"非アルコール性脂肪性肝炎：20症例\n（日本人）","en":"NASH: 20 cases\n(Japanese)"},"typeOfData":{"ja":"メタゲノム","en":"Metagenome"}},"releaseNote":{"ja":"非アルコール性脂肪性肝炎20症例の糞便から抽出したDNAを用いた16Sメタゲノム解析の結果をfastqファイルにて提供する。","en":"DNAs extracted from the stool samples collected from 20 patients with nonalcoholic steatohepatitis (NASH) were used for 16S metagenomic analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"建石 良介","en":"Ryosuke Tateishi"},"organization":{"name":{"ja":"東京大学医学部附属病院 消化器内科","en":"Gastroenterology, The University of Tokyo Hospital"}}}],"researchProjects":[{"name":{"ja":"肝臓疾患に関与する遺伝子とタンパク質の探索に関する研究","en":"Research on genes and proteins associated with liver disease."},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"ウイルス性肝疾患を含む代謝関連肝がん発生の病態解明に関する研究","en":"Study on the pathogenesis of metabolism-associated liver cancer including those associated with viral hepatitis"},"agency":{"ja":"日本医療研究開発機構（AMED） 肝炎等克服実用化研究事業","en":"Research Program on Hepatitis, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP20fk0210040"]}],"relatedPublications":[{"title":"Gut insulin action protects from hepatocarcinogenesis in diabetic mice comorbid with nonalcoholic steatohepatitis","doi":"https://doi.org/10.1038/s41467-023-42334-y","datasets":["JGAD000700"]}],"datasets":["JGAD000700"],"controlledAccessUsers":[]}