{"id":"hum0366","version":1,"url":"https://humandbs.dbcls.jp/research/hum0366/v1","datePublished":"2023-03-16","versions":[{"version":1,"datePublished":"2023-03-16"}],"title":{"ja":"新型コロナウイルス感染症(COVID-19)の病態解明","en":"Pathogenesis of severe acute respiratory syndrome coronavirus 2 infection (COVID-19)"},"summary":{"aims":{"ja":"新型コロナウイルス感染症（COVID-19）の病態生理及びコロナワクチン接種後の免疫動態を解析し、臨床分野での応用を目指す。","en":"To analyze the immune response to the mRNA vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and pathophysiology of the coronavirus disease 2019 (COVID-19) and to provide insights into the clinical development."},"methods":{"ja":"single cell RNA-seq：COVID-19患者16症例（ARDS合併；8症例、ARDS非合併；8症例）と対照健常者5名の末梢血単核球をシングルセルRNA sequenceで解析。\nRNA-seq：健常者4名の末梢血単核球由来の単球から抽出したtotal RNAをRNA sequenceで解析。\nATAC-seq：健常者5名の末梢血単核球由来の単球から抽出したDNAをATAC sequenceで解析。","en":"single cell RNA-seq: Peripheral blood mononuclear cells from COVID-19 patients (with ARDS, n=8; without ARDS, n=8) and healthy controls (n=5) were analyzed by single cell RNA sequence.\nRNA-seq: Total RNA extracted from monocytes derived from peripheral blood mononuclear cells of healthy subjects (n=4) was analyzed by RNA sequence.\nATAC-seq: DNA extracted from monocytes derived from peripheral blood mononuclear cells of healthy subjects (n=5) was analyzed by ATAC sequence."},"targets":{"ja":"single cell RNA-seq: COVID-19患者: 16症例（ARDS合併；8例、ADRS非合併；8例）、対照健常者: 5名\nRNA-seq：健常者4名（各4検体、いずれも末梢血）\nATAC-seq：健常者5名（各4ないしは5検体、いずれも末梢血）","en":"single cell RNA-seq: patients with COVID-19 (with ARDS, n=8; without ADRS, n=8), healthy subjects (n=5)\nRNA-seq: healthy subjects (n=4 per person, PBMC)\nATAC-seq: healthy subjects (n=4 or 5 per person, PBMC)"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\nクロマチン構造","en":"Expression profiling, Chromatin structure analysis"},"targets":{"ja":"COVID-19：16症例\n健常者：10症例\n（日本人）","en":"COVID-19: 16 cases\n10 healthy individuals\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq、RNA-seq、ATAC-seq）","en":"NGS\n(scRNA-seq, RNA-seq, ATAC-seq)"}},"releaseNote":{"ja":"mRNAワクチンを接種した健常者ならびにCOVID-19感染者の末梢血単核球から抽出したDNA/RNAを用いたscRNA-seq、RNA-seq、ATAC-seq解析のリードカウントデータおよびオープンクロマチン領域データを提供する（txt）。","en":"DNA/RNA extracted from PBMCs of COVID-19 patients and healthy subjects vaccinated with mRNA vaccine were used for scRNA-seq, RNA-seq, and ATAC-seq analyses. The read count data and open chromatin region data (text file) are provided."},"dataProviders":[{"name":{"ja":"加藤 保宏","en":"Yasuhiro Kato"},"organization":{"name":{"ja":"大阪大学大学院医学系研究科 呼吸器・免疫内科学","en":"Department of Respiratory Medicine and Clinical Immunology, Graduate School of Medicine, Osaka University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"神経・免疫・代謝におけるガイダンス因子の病的意義の解明とその制御","en":"Pathological significance and regulation of guidance factors in neurology, immunity and metabolism."},"agency":{"ja":"科学研究費助成事業 基盤研究（S）","en":"KAKENHI Grant-in-Aid for Scientific Research (S)"},"grantIds":["18H05282"]}],"relatedPublications":[{"title":"Consecutive BNT162b2 mRNA vaccination induces short-term epigenetic memory in innate immune cells","doi":"https://doi.org/10.1172/jci.insight.163347","datasets":["E-GEAD-551","E-GEAD-552"]}],"datasets":["E-GEAD-551","E-GEAD-552"],"controlledAccessUsers":[]}