{"id":"hum0358","version":1,"url":"https://humandbs.dbcls.jp/research/hum0358/v1","datePublished":"2022-12-20","versions":[{"version":1,"datePublished":"2022-12-20"},{"version":2,"datePublished":"2023-10-19"},{"version":3,"datePublished":"2025-09-24"},{"version":4,"datePublished":"2026-08-20"}],"title":{"ja":"子宮頸部腺癌・扁平上皮癌、腟・外陰癌の発生及び予後決定に関わる遺伝子異常の検索","en":"Research of genetic abnormalities related to the development and prognosis of adenocarcinoma and squamous cell carcinoma of the cervix and vaginal and vulvar cancer"},"summary":{"aims":{"ja":"現在、子宮頸部腺がんのような治療抵抗性の悪性腫瘍の発生及び予後決定に関わる明確な因子は同定されていない。本研究において、子宮頸部腺がんの分子生物学的特徴を、その他の子宮頸がん（扁平上皮がん）や腟・ 外陰部がんの発がんの分子生物学的特徴と対比した上で、子宮頸部腺がんの発生や予後決定に関わる分子マーカーを同定する。子宮頸部扁平上皮がんの中には若年発症かつ予後不良のがんも存在し、子宮頸部小細胞がんも進行が速く予後不良であることから、診断法・治療法の確立は急務である。","en":"To identify molecular markers involved in the development and prognosis of cervical adenocarcinoma, we would like to elucidate the molecular biological characteristics of cervical adenocarcinoma in relation to the carcinogenesis of other cervical cancers (squamous cell carcinoma), vaginal and vulvar cancers. In addition, some squamous cell carcinomas of the cervix develop at a young age and have a poor prognosis. Furthermore, small cell carcinoma of the uterine cervix also progresses rapidly and has a poor prognosis, making the establishment of diagnostic and therapeutic methods an urgent task."},"methods":{"ja":"全エクソン解析、RNAシークエンス解析、シングルセルRNAシークエンス解析、ターゲットバイサルファイトシークエンス解析","en":"Whole exome sequencing, RNA-seq, single-cell RNA sequencing, Target bisulfite sequencing"},"targets":{"ja":"子宮頸がん19＋4＋96症例、NIKS18細胞株\n・全エクソンシークエンス：1症例由来の腫瘍組織・腫瘍組織由来オルガノイド・末梢血各1検体、4症例由来の腫瘍組織（8検体）・末梢血（2検体）・腫瘍近傍の正常組織（2検体）、96症例由来の腫瘍組織（96検体）\n・RNA-seq：1症例由来の腫瘍組織・腫瘍由来オルガノイドの2検体、13症例由来の腫瘍組織（13検体）、5症例由来の同一腫瘍内複数検体（77検体）、3症例由来の正常子宮頸部由来オルガノイド（3検体）、1症例由来の正常子宮頸部マイクロダイセクションで取得した4検体、NIKS18細胞株に対してsiRNA knockdownを行ったサンプル5検体\n・single-cell RNA-seq：1症例由来の正常子宮頸部由来オルガノイド\n・ターゲットバイサルファイトシークエンス：96症例由来の腫瘍組織（96検体）","en":"cervical cancer total of 19 + 4 + 96 cases, NIKS18 cell line\n[Whole exome sequencing] 1 case: 3 samples of tumor/tumor-derived organoids/peripheral blood cell, 4 cases: 8 tumor samples, 2 peripheral blood samples, 2 normal areas near the tumors, 96 cases: 96 tumor samples\n[RNA-seq] 1 case: 2 samples of tumor/tumor-derived organoids, 13 cases: 13 tumor samples, 5 cases: multiple sampling from within the same tumor for a total of 77 samples, 3 cases: 3 samples from normal cervical tissue-derived organoids, 1 case: 4 samples from normal cervical tissue, siRNA-mediated knockdown samples of NIKS18 cell line (siNPM3: 2 samples, siNC: 3 samples)\n[single-cell RNA-seq] 1 case: 50cells of normal cervical tissue-derived organoids\n[Target bisulfite-seq] 96 cases: 96 tumor samples"},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"子宮頸部腺がんの腫瘍組織、腫瘍組織由来オルガノイド、および、非腫瘍組織（末梢血、腫瘍近傍の正常組織）から抽出したDNA/RNAを用いたwhole exome sequencingならびにRNA-seq解析データをfastq、bamファイル形式で提供する。","en":"DNAs/RNAs extracted from tumors, tumor-derived organoids, peripheral blood cells, or normal areas near the tumor of cervical cancer patients were used for the whole exome sequencing and RNA sequencing analyses. Fastq and bam files are provided."},"dataProviders":[{"name":{"ja":"曾根 献文","en":"Kenbun Sone"},"organization":{"name":{"ja":"東京大学医学部附属病院 女性診療科・産科","en":"Department of Obstetrics and Gynecology, The University of Tokyo Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"新規培養技術を用いた、扁平腺接合部細胞における高悪性度HPV18型の潜伏持続感染および発癌機構の解明","en":"Study of HPV-18 persistent infection and carcinogenesis in the squamocolumnar junction cells using novel culture technologies"},"agency":{"ja":"日本医療研究開発機構（AMED） 新興・再興感染症研究基盤創生事業","en":"Japan Program for Infectious Diseases Research and Infrastructure, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP22wm0325014"]},{"title":{"ja":"創薬等支援のための1細胞・微小生体組織のトランスクリプトーム解析","en":"Transcriptome analysis of single cell and micro tissue sections for supporting of drug discovery"},"agency":{"ja":"日本医療研究開発機構（AMED） 創薬等ライフサイエンス研究支援基盤事業 創薬等先端技術支援基盤プラットフォーム（BINDS）","en":"Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP21am0101104"]},{"title":{"ja":"単一細胞解析技術の統合によるHPV18型幹細胞発癌機構の解明","en":"Elucidation of stem cell carcinogenesis mechanism of HPV18-associated cervical cancer by integration of single-cell analysis technologies Japan Agency for Medical Research and Development"},"agency":{"ja":"日本医療研究開発機構（AMED） 新興・再興感染症研究基盤創生事業","en":"Japan Program for Infectious Diseases Research and Infrastructure, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP24wm0325057"]},{"title":{"ja":"子宮頸癌の起源細胞の同定と、発癌・分化調整機構の解明","en":"Identification of the cell of origin of uterine cervical cancer and elucidation of the regulatory mechanism for carcinogenesis and differentiation"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"KAKENHI Grant-in-Aid for Early-Career Scientists"},"grantIds":["22K16853"]},{"title":{"ja":"1細胞/微小組織マルチオミックスのオールインワン解析による生命科学研究の支援","en":"Support of life science studies by all-in-one single cell/micro tissue multi-omics analysis"},"agency":{"ja":"日本医療研究開発機構（AMED） 創薬等ライフサイエンス研究支援基盤事業 創薬等先端技術支援基盤プラットフォーム（BINDS）","en":"Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP25ama121055"]}],"relatedPublications":[{"title":"Cells with stem-like properties are associated with the development of HPV18-positive cervical cancer","doi":"https://doi.org/10.1111/cas.15664","datasets":["JGAD000708"]},{"title":"Application of organoid culture from HPV18-positive small cell carcinoma of the uterine cervix for precision medicine","doi":"https://doi.org/10.1002/cam4.5588","datasets":["JGAD000713"]}],"datasets":["JGAD000708","JGAD000713"],"controlledAccessUsers":[]}