{"id":"hum0357","version":6,"url":"https://humandbs.dbcls.jp/research/hum0357/v6","datePublished":"2025-04-28","versions":[{"version":1,"datePublished":"2022-11-30"},{"version":2,"datePublished":"2023-01-11"},{"version":3,"datePublished":"2023-02-15"},{"version":4,"datePublished":"2023-08-09"},{"version":5,"datePublished":"2024-06-05"},{"version":6,"datePublished":"2025-04-28"}],"title":{"ja":"肺癌の遺伝子発現解析の生物学的特性ならびに診断・治療におけるバイオマーカーの研究","en":"Research for biological features by gene expression analysis and biomarkers at diagnosis/therapeutics in lung cancer"},"summary":{"aims":{"ja":"呼吸器疾患症例の肺組織を用いたマルチオミックス解析による新規治療方法の解明","en":"To develop new treatments for pulmonary diseases by using NGS analyses data"},"methods":{"ja":"肺がん症例の腫瘍組織（胸水、喀痰、末梢血、摘出組織、生検）または、腫瘍組織周辺の正常肺組織から樹立したオルガノイドより抽出したDNA/RNAを用いたWhole Genome Sequencing（WGS）、Whole Exome Sequencing（WES）、RNA-seq、scRNA-seq、ATAC-seq、Methylation解析","en":"Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), ATAC-seq, and methylation array analyses with DNA harvested from lung cancer organoids established from surgery, biopsy, pleural effusion, sputum, and peripheral blood samples of patients. RNA-seq and single cell RNA-seq analyses with RNA harvested from lung cancer organoids established from surgery, biopsy, pleural effusion, sputum, normal lung organoids and peripheral blood samples of patients."},"targets":{"ja":"肺がん43症例43検体（JGAS000557/JGAD000678）＋33症例40検体（JGAS000609/JGAD000738）＋31症例42検体（JGAS000610/JGAD000739）","en":"Lung cancer"},"url":{"ja":[{"url":"https://www.keio-med.jp/pulmonary/clinical/case.html","text":"https://www.keio-med.jp/pulmonary/clinical/case.html"}],"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現\nメチル化","en":"Sequencing, Expression profiling, Methylation profiling"},"targets":{"ja":"肺癌：105＋2症例\n（日本人）","en":"lung cancer: 105 + 2 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（WGS、Exome、RNA-seq、scRNA-seq、ATAC-seq）\nメチル化アレイ","en":"NGS\n(WGS, Exome, RNA-seq, scRNA-seq, ATAC-seq)\nMethylation array"}},"releaseNote":{"ja":"肺がん3症例の腫瘍組織から樹立したオルガノイド3検体を用いたWES解析データ（3症例3検体、内、新規2症例2検体）およびRNA-seq解析データ（3症例3検体、内、新規2症例2検体）ならびにsingle cell RNA-seq解析データ（8症例8検体、新規なし）をfastqファイルにて提供する。","en":"DNAs/RNAs were extracted from organoids established from 3 tumor tissues of 3 patients (JGAS000610/JGAD000739) with lung cancer. WES, RNA-seq, and scRNA-seq analyses were performed. Fastq files are provided."},"dataProviders":[{"name":{"ja":"安田 浩之","en":"Hiroyuki Yasuda"},"organization":{"name":{"ja":"慶應義塾大学 医学部 呼吸器内科","en":"Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"肺癌オルガノイドライブラリー統合解析による癌の不均一性の解明と新規治療標的同定","en":"Clarification of heterogeneity of lung cancer and identification of novel therapeutic targets using lung cancer organoid library"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP21cm0106576"]},{"title":{"ja":"肺癌オルガノイドライブラリーを用いた肺癌フェノタイプ多様性を規定する分子基盤解明と新規治療標的同定","en":"Clarification of molecular mechanisms creating phenotypic diversity of lung cancer using patient-derived organoid library"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的がん医療実用化研究事業","en":"Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP22ck0106722"]},{"title":{"ja":"肺癌オルガノイドライブラリーを用いた肺癌シグナル経路異常の解明","en":"Clarification of signal alteration mechanisims of lung cancer using lung cancer organoid library"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["21H02765"]},{"title":{"ja":"肺癌オルガノイドライブラリーを用いた新規治療標的の同定","en":"Establishment of lung cancer and normal lung organoid library"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K08610"]},{"title":{"ja":"小細胞肺癌オルガノイドを用いた小細胞肺癌発癌過程の理解","en":"Clarification of carcinogenesis process of small cell lung cancer using patient-derived organoid library"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["22K08290"]}],"relatedPublications":[{"title":"Genotype-phenotype mapping of a patient-derived lung cancer organoid biobank identifies NKX2-1-defined Wnt dependency in lung adenocarcinoma","doi":"https://doi.org/10.1016/j.celrep.2023.112212","datasets":["JGAD000678"]},{"title":"An organoid library unveils subtype-specific IGF-1 dependency via a YAP-AP1 axis in human small cell lung cancer","doi":"https://doi.org/10.1038/s43018-025-00945-y","datasets":["JGAD000738"]},{"title":"Basal-shift transformation leads to EGFR therapy-resistance in human lung adenocarcinoma","doi":"https://doi.org/10.1038/s41467-025-59623-3","datasets":["JGAD000739"]}],"datasets":["JGAD000678","JGAD000738","JGAD000739"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"安田 浩之","en":"Hiroyuki Yasuda"},"affiliation":{"ja":"医学部内科学（呼吸器）, 慶應義塾大学","en":"Department of Pulmonary Medicine, Keio University School of Medicine"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"肺癌の遺伝子発現解析の生物学的特性ならびに診断・治療におけるバイオマーカーの研究","en":"Research for biological features by gene expression analysis and biomarkers at diagnosis/therapeutics in lung cancer"},"periodStart":"2025-05-20","periodEnd":"2028-03-31","datasets":["JGAD000678"]},{"principalInvestigator":{"en":"David Olagnier"},"affiliation":{"en":"Aarhus University"},"country":{},"researchTitle":{"en":"KEAP1 mutation creates a niche for oncovirotherapy by impairing constitute antiviral immunity in lung cancer\r\n"},"periodStart":"2025-12-01","periodEnd":"2027-01-01","datasets":["JGAD000678"]}]}