{"id":"hum0345","version":1,"url":"https://humandbs.dbcls.jp/research/hum0345/v1","datePublished":"2022-08-05","versions":[{"version":1,"datePublished":"2022-08-05"}],"title":{"ja":"臍帯血を用いた再生医療に向けた基礎的研究","en":"Fundamental study for regenerative medicine utilizing cord blood"},"summary":{"aims":{"ja":"リンパ球系および樹状細胞の分化経路およびそのメカニズムをヒト臍帯血を用いて解明する","en":"To clarify the differentiation pathway and mechanism of human lymphoid and dendritic cells from hematopoietic stem cells"},"methods":{"ja":"臍帯血中のCD34陽性細胞、あるいは、CD34陽性細胞中のリンパ球系分画をソート後にストローマ細胞と共培養し、抽出したRNA/DNAを用いたsingle cell RNA-seqおよびATAC sequence解析。","en":"CD34+ cells or target cells in CD34+ cells were sorted and assessed for scRNA-seq and ATAC-seq analyses. Obtained findings were evaluated by culture on a co-culture system."},"targets":{"ja":"健常者の正常分娩後、臍帯より臍帯血 30～50 ml を採取し、2日以内にCD34 陽性細胞を分離・保存する。\n・研究協力者2名の臍帯血を混合後ソートしたCD34陽性細胞から抽出したDNA/RNA\n・研究協力者30名分の臍帯血を混合後ソートしたCD10SP細胞から抽出したRNA","en":"Cord blood (30-50ml) was obtained from healthy volunteers and kept in a freezer after the selection of CD34+ cells. Mixtures of several samples were used for experiments.\n- One mixed sample (CD34+) of 2 volunteers' cord blood\n- One mixed sample (CD10SP) of 30 volunteers' cord blood"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\nクロマチン構造","en":"Expression profiling, Chromatin structure profiling"},"targets":{"ja":"健常者：30名\n（日本人）","en":"30 healthy volunteers"},"typeOfData":{"ja":"NGS\n（scRNA-seq、ATAC-seq）","en":"NGS\n(scRNA-seq, ATAC-seq)"}},"releaseNote":{"ja":"健常人2名の臍帯血より分取したCD34陽性細胞から抽出したRNA/DNAを対象としたscRNA-seqならびにATAC-seqを行った結果、および健常人30名の臍帯血より分取したCD10SP分画から抽出したRNAを対象としたscRNA-seqを行った結果をfastqファイルにて提供する。","en":"RNA/DNA extracted from CD34+ cells or lymphoid cells (CD10SP) from volunteers' cord blood were used for scRNA-seq and ATAC-seq analyses. Fastq files were provided."},"dataProviders":[{"name":{"ja":"大石 晃嗣","en":"Kohshi Ohishi"},"organization":{"name":{"ja":"三重大学医学部附属病院 輸血・細胞治療部","en":"Transfusion Medicine and Cell Therapy, Mie University Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"新規培養法を用いたヒトリンパ球系分化制御および腫瘍化の分子遺伝学的研究","en":"Study of human lymphopoiesis and neoplastic transformation with novel culture system"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["18K08322"]},{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Science"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["16H06279"]}],"relatedPublications":[],"datasets":["JGAD000647","JGAD000671"],"controlledAccessUsers":[]}