{"id":"hum0342","version":3,"url":"https://humandbs.dbcls.jp/research/hum0342/v3","datePublished":"2026-01-26","versions":[{"version":1,"datePublished":"2022-06-27"},{"version":2,"datePublished":"2026-01-05"},{"version":3,"datePublished":"2026-01-26"}],"title":{"ja":"消化器腫瘍の免疫組織化学的・分子病理学的検索","en":"Immunohistochemical and molecular pathological search in gastrointestinal tumors"},"summary":{"aims":{"ja":"近年の腫瘍における遺伝子変異・発現解析の進展により、種々の消化器腫瘍における分子病理学的背景が明らかにされつつある。今後、このような検索をさらに進めていくとともに、これらの知見を組織病理診断の精度向上や、悪性度診断等に結びつけていく事が期待される。 本研究では主に消化器腫瘍を対象として、遺伝子変異・発現および蛋白発現の統合的な解析を行う。これらの所見と病理組織所見および臨床病理学的所見との相関を明らかにする事で、組織病理診断や分子病理学診断に応用可能な、新たな組織型特異的マーカー・予後因子の探索を行う。","en":"Recent advances in somatic mutation and expression analysis in tumors have revealed the molecular pathological background of various gastrointestinal tumors. In the future, it is expected that these findings will be used to improve the accuracy of histopathological and grade diagnoses, as well as to further advance such searches. In this study, we conducted an integrated analysis of somatic mutations, gene expression, and protein expression, mainly in gastrointestinal tumors. By correlating these findings with histopathological and clinicopathological findings, we will search for new tissue-specific markers and prognostic factors that can be applied to histopathological and molecular pathological diagnoses."},"methods":{"ja":"RSPO融合陽性大腸癌症例に対して全エクソンシークエンス解析を実施した。小腸癌症例に対して、全エクソンシークエンス解析ならびにトランスクリプトーム解析を実施した。StageIII期の大腸がん症例に対して、トランスクリプトーム解析を実施した。","en":"Whole exome sequencing (WES) was conducted using tumor and non-tumor tissues obtained from RSPO fusion-positive cases. Whole exome sequencing (WES) and transcriptome analysis (RNA-seq) were conducted using tumor and non-tumor tissues obtained from small intestine cancer cases. Also RNA-seq were conducted using tumor obtained from colorectal cancer cases."},"targets":{"ja":"大腸がん、小腸がん","en":"colorectal cancer, small intestine cancer"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"大腸癌：29＋253症例\n小腸癌：22症例\n（日本人）","en":"Colorectal cancer: 29 + 253 cases\nSmall intestine cancer: 22 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq）","en":"NGS\n(Exome, RNA-seq)"}},"releaseNote":{"ja":"大腸がん253症例の腫瘍組織より抽出したRNAを用いたRNA sequencing解析データをfastqファイルにて提供する。","en":"RNA extracted from tumor tissues obtained from 253 colorectal cancer cases were used for RNA sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"関根 茂樹","en":"Shigeki Sekine"},"organization":{"name":{"ja":"国立研究開発法人国立がん研究センター中央病院 病理診断科","en":"Department of Diagnostic Pathology, National Cancer Center Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"RSPO融合遺伝子陽性大腸癌の臨床病理学的特徴の解析","en":"Clinicopathological analysis of RSPO fusion-positive colorectal carcinoma"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["20K07382"]},{"title":{"ja":"RNA in situ hybridization法を用いた大腸癌および胃癌におけるRSPO遺伝子融合/過剰発現の効率的な検出法の開発","en":"Efficient detection of RSPO gene fusion/overexpression in colorectal and gastric cancers using RNA in situ hybridization"},"agency":{"ja":"黒住医学研究振興財団研究助成","en":"Kurozumi Medical Foundation"},"grantIds":null}],"relatedPublications":[{"title":"Clinicopathological and molecular characteristics of RSPO fusion-positive colorectal cancer","doi":"https://doi.org/10.1038/s41416-022-01880-w","datasets":["JGAD000657"]}],"datasets":["JGAD000657","JGAD001011","JGAD001018"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000657"]}]}