{"id":"hum0337","version":1,"url":"https://humandbs.dbcls.jp/research/hum0337/v1","datePublished":"2024-07-19","versions":[{"version":1,"datePublished":"2024-07-19"}],"title":{"ja":"難治性婦人科悪性腫瘍の包括的オミックス解析に基づく新規テーラーメード治療の創出を目指した基盤的研究","en":"Fundamental research on establishment of a novel tailor-maid therapy for refractory gynecologic malignancy based on comprehensive omics analysis"},"summary":{"aims":{"ja":"卵巣成熟奇形腫の悪性転化に関わる特徴を一細胞レベルで明らかにする","en":"To investigate the characteristics of squamous cell carcinoma arising from mature teratoma of the ovary by single nucleus RNA sequencing."},"methods":{"ja":"新鮮凍結組織をホモジェナイズ後核分画を抽出し、10x Genomics社のChromium Next GEM Single Cell 3' Reagent Kits v3.1に沿ってライブラリー調整を行い、Illumina社のNovaseqにてRNAシーケンスを行った。","en":"Nuclei were isolated from archival fresh frozen cancer tissues and libraries were acquired with the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 following the manufacturer's protocol (10X Genomics). Then, libraries were sequenced on a Novaseq (Illumina)."},"targets":{"ja":"成熟奇形腫の悪性転化5症例の腫瘍組織","en":"Five patients with squamous cell carcinoma arising from mature teratoma of the ovary"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"悪性転化を伴う卵巣成熟奇形腫：5症例\n（日本人）","en":"squamous cell carcinoma arising from mature teratoma of the ovary: 5 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"卵巣成熟奇形腫の悪性転化5症例の腫瘍組織を用いたscRNA-seq解析データをfastqファイルにて提供する。","en":"RNAs extracted from tumor tissues from five patients with squamous cell carcinoma arising from mature teratoma of the ovary were used for single-cell RNA-sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"吉田 康将","en":"Kosuke Yoshida"},"organization":{"name":{"ja":"名古屋大学 大学院医学系研究科","en":"Nagoya University Graduate School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Sciences"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["16H06279"]}],"relatedPublications":[],"datasets":["JGAD000639"],"controlledAccessUsers":[]}