{"id":"hum0321","version":1,"url":"https://humandbs.dbcls.jp/research/hum0321/v1","datePublished":"2022-06-17","versions":[{"version":1,"datePublished":"2022-06-17"}],"title":{"ja":"胸腺組織を介した免疫系構築の遺伝子制御に関する研究","en":"Comprehensive analysis of interaction between human gene expression and environmental metagenomes"},"summary":{"aims":{"ja":"正常胸腺および自己免疫性疾患との関連が強く示唆されている胸腺腫・胸腺過形成組織における 胸腺上皮細胞・胸腺内免疫細胞の解析を行うとともに、自己免疫疾患罹患者の末梢血免疫細胞の特性解析、末梢血中および髄液中自己抗体産生解析を行うことで胸腺免疫システムの破綻と自己免疫疾患発症機序を解明する。本解析では通常の FACS 解析および single cell 解析も含む。免疫担当細胞の発生・分化および機能について、遺伝子発現の観点から胸腺制御による免疫細胞の応答について解析も試みる。本研究の成果としては、様々な自己免疫疾患における発症機序としての胸腺での免疫制御の鍵分子同定が可能となり、新規薬剤ターゲットの開発起点となることが期待できる。","en":"We analyze thymic epithelial cells and intrathymic immune cells in the normal thymus and in thymoma and thymic hyperplasia tissues, which have been strongly implicated in autoimmune diseases. These analyses include conventional FACS analysis and single-cell analysis. The development, differentiation, and function of immunocompetent cells will also be analyzed from the perspective of gene expression as well as thymic regulation of immune cell responses. The results of this research will enable the identification of key molecules in thymic immune regulation as a pathogenic mechanism in various autoimmune diseases and will serve as a starting point for the development of new drug targets."},"methods":{"ja":"血液および胸腺組織からRNAを抽出し、次世代シークエンサーによるsingle cell RNA-seq解析を行った。","en":"Blood cells and thymus tissues were collected and subjected to single-cell RNA sequencing using a next-generation sequencer."},"targets":{"ja":"重症筋無力症症例の末梢血または治療の際に切除された胸腺を解析対象とする。","en":"Peripheral blood samples from patients with myasthenia gravis, or thymus glands from the patients resected at the time of surgery were used for the analysis."},"url":{"ja":[{"url":"https://singlecell.broadinstitute.org/single_cell/study/SCP1532/thymoma-and-pbmc-of-myasthenia-gravis-patients#study-summary","text":"https://singlecell.broadinstitute.org/single_cell/study/SCP1532/thymoma-and-pbmc-of-myasthenia-gravis-patients#study-summary"}],"en":[{"url":"https://singlecell.broadinstitute.org/single_cell/study/SCP1532/thymoma-and-pbmc-of-myasthenia-gravis-patients#study-summary","text":"https://singlecell.broadinstitute.org/single_cell/study/SCP1532/thymoma-and-pbmc-of-myasthenia-gravis-patients#study-summary"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"重症筋無力症：4症例","en":"Myasthenia gravis: 4 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"重症筋無力症4症例の末梢血（2症例2検体）および生体組織（胸腺腫手術摘出検体4症例4検体）から抽出したRNAを用いたsingle cell RNA-seq解析データをfastqファイルにて提供する。","en":"RNAs extracted from peripheral blood cells or thymoma surgery of 4 myasthenia gravis patients were used for single-cell RNA-sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"奥野 龍禎","en":"Tatsusada Okuno"},"organization":{"name":{"ja":"大阪大学大学院 医学系研究科 神経内科学","en":"Department of Neurology, Osaka University Graduate School of Medicine"}}}],"researchProjects":[{"name":{"ja":"神経免疫グループ","en":"Neuroimmunology Group"},"url":{"ja":[{"url":"https://www.med.osaka-u.ac.jp/pub/neurol/myweb6/group03.html","text":"https://www.med.osaka-u.ac.jp/pub/neurol/myweb6/group03.html"}],"en":null}}],"grants":[{"title":{"ja":"制御性T細胞による免疫応答制御の包括的研究","en":"Study of the function and development of regulatory T cells"},"agency":{"ja":"科学研究費助成事業 特別推進研究","en":"KAKENHI Grant-in-Aid for Specially Promoted Research"},"grantIds":["16H06295"]},{"title":{"ja":"制御性T細胞を標的とした免疫応答制御技術に関する研究開発","en":"Study of Immunoregulatory technology targeting regulatory T cells"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的先端研究開発支援事業","en":"Innovation and Clinical Research Center Project, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP20gm0010005"]}],"relatedPublications":[{"title":"Myasthenia gravis-specific aberrant neuromuscular gene expression by medullary thymic epithelial cells in thymoma","doi":"https://doi.org/10.1038/s41467-022-31951-8","datasets":["JGAD000599"]}],"datasets":["JGAD000599"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000599"]},{"principalInvestigator":{"ja":"岡田 随象","en":"Yukinori Okada"},"affiliation":{"ja":"遺伝統計学, 大阪大学大学院医学系研究科","en":"Department of Statistical Genetics, Graduate School of Medicine, Osaka University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"多層的オミクス解析による疾患病態の解明","en":"Elucidation of disease etiology by trans-layer omics analysis"},"periodStart":"2024-03-05","periodEnd":"2029-03-31","datasets":["JGAD000599"]}]}