{"id":"hum0320","version":1,"url":"https://humandbs.dbcls.jp/research/hum0320/v1","datePublished":"2022-01-25","versions":[{"version":1,"datePublished":"2022-01-25"}],"title":{"ja":"悪性腫瘍を有する患者を対象とした免疫モニタリング研究","en":"Immunomonitoring study in patients with malignant tumors"},"summary":{"aims":{"ja":"悪性腫瘍を有する患者における末梢血や腫瘍組織等での種々の免疫担当細胞や免疫担当因子などを解析し、その免疫学的状態を明らかにする。副次的に免疫状態と臨床病理学的特徴、分子生物学的特徴、治療効果や予後との関連について検討する。","en":"Various immunocompetent cells and factors in the peripheral blood cells and tumor tissues of patients with malignant tumors were analyzed. The immunological status of patients with malignant tumors will be clarified by analyzing various immune cells and factors in peripheral blood cells and tumor tissues. The relationship between immune status and clinicopathological characteristics, molecular biological characteristics, therapeutic effects, and prognosis will be evaluated as well."},"methods":{"ja":"免疫担当細胞（CD4/CD8T細胞、抗原提示細胞、制御性T細胞、 MDSC、自然リンパ球など）におけるCD4, CD8, FoxP3, CD25, CD45RA, CTLA-4, PD-1, ICOS, BTLA, Tim-3, LAG3, CCR7などの分子発現について、シングルセルシークエンスを用いた免疫フェノタイプ解析を実施した。併せて、免疫細胞のT-cell receptor（TCR）の解析を実施した。","en":"Expressions of CD4, CD8, FoxP3, CD25, CD45RA, CTLA-4, PD-1, ICOS, BTLA, Tim-3, LAG3, CCR7, and other molecules on immunocompetent cells (CD4/CD8 T cells, antigen-presenting cells, regulatory T cells, MDSCs, spontaneous lymphocytes, etc.) were analyzed by single-cell sequencing technology. The T-cell receptor (TCR) of immune cells was also analyzed in the same technology."},"targets":{"ja":"肺腺がん2症例および頭頸部がん3症例（腫瘍組織1検体、リンパ節1検体、末梢血1検体、計15検体）","en":"pulmonary gland cancer: 2 cases, head and neck part cancer: 3 cases"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"肺腺癌：2症例\n頭頸部癌：3症例\n（日本人）","en":"lung adenocarcinoma: 2 cases\nhead and neck cancer: 3 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq、TCR-seq）","en":"NGS\n(scRNA-seq, TCR-seq)"}},"releaseNote":{"ja":"肺腺がん2症例、頭頸部がん3症例の腫瘍組織、リンパ節、および末梢血から単離したT細胞から抽出したRNAを用いたscRNA-seqならびにTCR-seq解析の結果をfastqファイル形式で提供する。","en":"RNAs extracted from a single T cell isolated from tumor tissues, lymph nodes, and peripheral blood cells of lung adenocarcinoma and head and neck cancer patients were used for the single-cell RNA sequencing and TCR sequencing analyses. Fastq files are provided."},"dataProviders":[{"name":{"ja":"冨樫 庸介","en":"Yosuke Togashi"},"organization":{"name":{"ja":"千葉県がんセンター研究所","en":"Chiba Cancer Center Research Institute"}}}],"researchProjects":[],"grants":[{"title":{"ja":"がん抗原階層性からの腫瘍浸潤PD-1陽性T細胞の抗腫瘍免疫応答及び抑制能の解明","en":"Analysis of PD-1+ tumor-infiltrating T cells according to cancer antigen hierarchy"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["20H03694"]},{"title":{"ja":"シングルセルシークエンスによるネオ抗原特異的T細胞の時空間的解析から治療標的・バイオマーカーへの応用","en":"Spatial and temporal analysis of neoantigen-specific T-cell clones as a therapeutic target and biomarker"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的がん医療実用化研究事業","en":"Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP19ck0106521"]},{"title":{"ja":"T細胞受容体認識エピトープによる腫瘍浸潤Tリンパ球の次世代解析方法の開発","en":"Novel tumor-infiltrating T cell analysis according to antigen epitope"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP18cm0106340"]},{"title":{"ja":"ゲノム異常を有する腫瘍浸潤リンパ球の1細胞解析方法の開発とその臨床的意義の解明","en":"Development of a single-cell analysis method for tumor-infiltrating lymphocytes with genomic abnormalities and elucidation of its clinical significance"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP21cm0106383"]}],"relatedPublications":[{"title":"PD-1 blockade therapy promotes infiltration of tumor-attacking exhausted T cell clonotypes","doi":"https://doi.org/10.1016/j.celrep.2022.110331","datasets":["JGAD000597"]}],"datasets":["JGAD000597"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Ansuman Satpathy"},"affiliation":{"en":"Department of Pathology, Stanford University"},"country":{"ja":"アメリカ合衆国","en":"United States"},"researchTitle":{"en":"Epigenetics of Inflammatory Skin Disorders"},"periodStart":"2022-07-13","periodEnd":"2023-11-06","datasets":["JGAD000597"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000597"]}]}