{"id":"hum0317","version":1,"url":"https://humandbs.dbcls.jp/research/hum0317/v1","datePublished":"2021-12-06","versions":[{"version":1,"datePublished":"2021-12-06"},{"version":2,"datePublished":"2024-06-20"},{"version":3,"datePublished":"2025-11-18"}],"title":{"ja":"創薬研究に有用な患者検体移植モデルの構築に関する研究","en":"Research on the establishment of patient derived xenograft models useful for drug discovery research"},"summary":{"aims":{"ja":"外科的切除、放射線治療、抗がん剤治療を検討している、または既に治療を開始しているがん患者の腫瘍組織を用いたPatient-Derived Xenograft（PDX）モデルを作製し、ヒト生体組織に近似した形での抗悪性腫瘍薬の薬物動態・治療効果解析研究を行う。","en":"To analyze the pharmacokinetics and therapeutic effects of anticancer drugs in patients with cancer who are undergoing or have already begun surgical resection, radiotherapy, or anticancer drug therapy by creating a patient-derived xenograft (PDX) model using patient specimens to approximate human biological tissues. This is to create a PDX model using patient specimens to analyze the pharmacokinetics and therapeutic effects of anticancer drugs in a manner that approximates human tissues."},"methods":{"ja":"患者由来の腫瘍組織、ならびに、PDX由来の腫瘍組織からDNA・RNAを抽出し、whole exome sequencing解析ならびにRNA-seq解析を実施した。","en":"Whole-exome sequencing and RNA-sequencing analyses"},"targets":{"ja":"- ドライバー遺伝子異常を持つ非小細胞肺がん4症例（計33検体）\n- 何らかの腫瘍を保有する52＋269症例（生殖細胞系列細胞44＋269検体、PDX腫瘍組織52＋269検体、患者腫瘍組織125検体）","en":"- Non-small cell lung cancer with driver gene abnormalities 4 cases (33 samples)\n- 52 + 269 cases with any cancer (germline tissues:44 + 269 samples, tumor tissues from PDX: 52 + 795 samples, tumor tissues from patients: 125 samples)"},"url":{"ja":[{"url":"https://www.ncc.go.jp/jp/ri/department/pharmacology_therapeutics/project/040/index.html","text":"https://www.ncc.go.jp/jp/ri/department/pharmacology_therapeutics/project/040/index.html"}],"en":[{"url":"https://www.ncc.go.jp/en/ri/department/pharmacology_therapeutics/index.html","text":"https://www.ncc.go.jp/en/ri/department/pharmacology_therapeutics/index.html"}]}},"listingSummary":{},"releaseNote":{"ja":"非小細胞肺がん症例の生殖細胞系列細胞、腫瘍組織およびPDX腫瘍から抽出したDNA、RNAを用いたwhole exome sequencing解析ならびにRNA-seq解析の結果をbamおよびfastqファイル形式で提供する。","en":"DNAs/RNAs extracted from germline tissues and tumor tissues from patients with non-small cell lung cancer and tumor tissues from PDX using patient specimens were used for the whole exome sequencing and RNA sequencing analyses. Bam and fastq files are provided."},"dataProviders":[{"name":{"ja":"濱田 哲暢","en":"Akinobu Hamada"},"organization":{"name":{"ja":"国立がん研究センター研究所 分子薬理研究分野","en":"Division of Molecular Pharmacology, National Cancer Center Research Institute"}}}],"researchProjects":[{"name":{"ja":"J-PDXライブラリープロジェクト","en":"J-PDX library project"},"url":{"ja":[{"url":"https://www.ncc.go.jp/jp/ri/department/pharmacology_therapeutics/project/040/index.html","text":"https://www.ncc.go.jp/jp/ri/department/pharmacology_therapeutics/project/040/index.html"}],"en":[{"url":"https://www.ncc.go.jp/en/ri/department/pharmacology_therapeutics/index.html","text":"https://www.ncc.go.jp/en/ri/department/pharmacology_therapeutics/index.html"}]}}],"grants":[{"title":{"ja":"がん医療推進のための日本人がん患者由来PDXライブラリー整備事業","en":"Project to develop a PDX library derived from Japanese cancer patients for the promotion of cancer treatment"},"agency":{"ja":"日本医療研究開発機構（AMED） 医療研究開発革新基盤創成事業（CiCLE）","en":"Cyclic Innovation for Clinical Empowerment (CiCLE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP17pc0101011"]},{"title":{"ja":"ヒト腫瘍内多様性を正しく反映するPDX樹立手法の開発","en":"Development of a method to establish PDX that correctly reflects the diversity within human tumors"},"agency":{"ja":"国立がん研究センターがん研究開発費","en":"National Cancer Center Research and Development Fund"},"grantIds":["31-A-3"]},{"title":{"ja":"PDXモデルを用いた非臨床試験の実施に係る標準化に関する研究","en":"Study on standardization of non-clinical studies using PDX model"},"agency":{"ja":"日本医療研究開発機構（AMED） 医薬品等規制調和・評価研究事業","en":"Research on Regulatory Science of Pharmaceuticals and Medical Devices, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP21mk0101181"]},{"title":{"ja":"遺伝子変異を有する非小細胞肺がんのPDXモデル樹立と新薬開発の基盤研究","en":"Establishment of PDX model for non-small cell lung cancer with genetic alteration and basic research for new drug development"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"KAKENHI Grant-in-Aid for Early-Career Scientists"},"grantIds":["18K15296"]},{"title":{"ja":"希少がんならびに難治性がんに対する抗がん剤治療開発を加速させる創薬研究手法に関する研究","en":"Study on methods for drug discovery research to accelerate the development of anticancer drugs for rare and refractory cancers"},"agency":{"ja":"日本医療研究開発機構（AMED） 医療研究開発革新基盤創成事業（CiCLE）","en":"Cyclic Innovation for Clinical Empowerment (CiCLE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP20pc0101051"]},{"title":{"ja":"AIを利用した新規抗がん薬の創薬研究加速システムの開発","en":"Development of an Artificial Intelligence-based system to accelerate drug discovery and research for new anti-cancer agents"},"agency":{"ja":"日本医療研究開発機構（AMED） ゲノム研究を創薬等出口に繋げる研究開発プログラム","en":"Research and development program leading genomic research to drug discovery and genomic medicine, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP23tk0124004"]},{"title":{"ja":"がんの本態解明から革新的な医療実用化に向けた一貫したマネジメントスキームの確立研究","en":"Research on Establishing a Consistent Management Scheme from Cancer Pathogenesis Elucidation to Innovative Medical Practical Application"},"agency":{"ja":"日本医療研究開発機構（AMED）革新的がん医療実用化研究事業","en":"Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP23ck0106771"]}],"relatedPublications":[{"title":"Comparative study on the efficacy and exposure of molecular target agents in non-small cell lung cancer PDX models with driver genetic alterations","doi":"https://doi.org/10.1158/1535-7163.MCT-21-0371","datasets":["JGAD000530"]},{"title":"A molecular glue RBM39-degrader induces synthetic lethality in cancer cells with homologous recombination repair deficiency","doi":"https://doi.org/10.1038/s41698-024-00610-0","datasets":["JGAD000840"]},{"title":"Comprehensive DNA Profiling Strategy for Patient-Derived Xenograft Model Authentication in the J-PDX Library","doi":"https://doi.org/10.1038/s41598-025-21299-6","datasets":["JGAD000995"]}],"datasets":["JGAD000530"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"中岡 博史","en":"Hirofumi Nakaoka"},"affiliation":{"ja":"腫瘍ゲノム研究部, 公益財団法人佐々木研究所","en":"Department of Cancer Genome Research, Sasaki Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"肺がん遺伝子解析研究","en":"Genetic analysis of lung cancer"},"periodStart":"2022-08-17","periodEnd":"2026-07-23","datasets":["JGAD000530"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000530"]}]}