{"id":"hum0305","version":1,"url":"https://humandbs.dbcls.jp/research/hum0305/v1","datePublished":"2021-11-29","versions":[{"version":1,"datePublished":"2021-11-29"}],"title":{"ja":"造血細胞移植後二次がんの細胞遺伝学的特徴に関する多施設後方視的研究","en":"A multicenter study of biological and genetic manifestations of subsequent solid cancers after hematopoietic cell transplantation"},"summary":{"aims":{"ja":"造血細胞移植後2次固形がんは、通常のがんと比較して、固有のゲノム異常プロファイルを有した腫瘍である可能性がある。2次固形がんと通常のがんの検体を対象とした全エクソン配列決定解析を行うことにより、我々の仮説を検証する。","en":"Subsequent solid cancers after allogenic hematopoietic cell transplantation may have unique genomic profiles compared with de novo cancers. In order to identify such profiles, we applied whole exome sequencing (WES) of subsequent solid cancers after allogeneic hematopoietic cell transplantation as well as de novo cancers."},"methods":{"ja":"ゲノムDNAは、凍結新鮮検体はQIAamp Fast DNA Tissue kit、FFPE検体はGeneRead DNA FFPE kitを用いて調整した。全エクソームシークエンス（Whole Exome Sequence：WES）解析用のライブラリはTwist社のTwist library preparation kitを用いて作製した。作製したライブラリはIllumina社 Novaseq 6000またはMGI社DNBSEQ-G400でシークエンス解析を行った。","en":"Genomic DNA was extracted using a QIAamp Fast DNA Tissue kit, GeneRead DNA FFPE kit or QIAamp DNA Mini kit. Libraries for WES were prepared using the Twist library preparation kit. The libraries were sequenced using a Novaseq 6000 or DNBSEQ G400 with the paired-end option."},"targets":{"ja":"固形がん14症例の腫瘍組織凍結検体14検体、および、ホルマリン固定パラフィン包埋（FFPE）14検体","en":"14 fresh-frozen tumor tissues and 14 formalin-fixed-paraffin-embedded (FFPE) tumor tissues of 14 de novo cancer patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"固形癌：14症例\n（日本人）","en":"14 de novo cancer patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"固形がん（舌がん、大腸がん）の腫瘍組織の凍結検体とFFPE検体から抽出したDNAを用いた全エクソームシークエンス解析の結果をbamファイル形式で提供する。","en":"DNAs extracted from fresh-frozen and FFPE tumor tissues from 14 de novo cancer patients were used for the whole exome sequencing analysis. Bam files are provided."},"dataProviders":[{"name":{"ja":"稲本 賢弘","en":"Yoshihiro Inamoto"},"organization":{"name":{"ja":"国立がん研究センター中央病院 造血幹細胞移植科","en":"Department of Hematopoietic Stem Cell Transplantation, National Cancer Center Hospital"}}}],"researchProjects":[{"name":{"ja":"造血細胞移植後2次固形がんのゲノム解析","en":"Comprehensive molecular profiling of subsequent solid cancers after allogenic hematopoietic cell transplantation"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"ゲノム解析に基づいた造血細胞移植後2次固形がん最適個別化医療の実現","en":"Establishment of personalized medicine for secondary solid tumors after hematopoietic cell transplantation based on genomic characterization"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的がん医療実用化研究事業","en":"Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP20ck0106539"]}],"relatedPublications":[{"title":"MicroSEC filters sequence errors for formalin-fixed and paraffin-embedded samples","doi":"https://doi.org/10.1038/s42003-021-02930-4","datasets":["JGAD000491"]}],"datasets":["JGAD000491"],"controlledAccessUsers":[]}